I{sup 131} therapy induces persistent radiation-dose dependent increases in glycophorin a locus somatic mutations in bone marrow stem cells
- Univ. of Pittsburgh, PA (United States)
- Univ. of California, San Francisco, CA (United States)
- Lawrence Livermore National Lab., CA (United States)
- National Institutes of Health, Bethesda, MD (United States)
- National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD (United States)
Patients with thyroid diseases treated with I{sup 131} receive known sub-acute marrow exposures to ionizing radiation of {approximately}2 to >200 cGy. Time-series sampling of peripheral blood from these patients, assayed for the frequency of erythrocytes expressing glycophorin A (GPA) allele-loss variant phenotypes, demonstrates the induction, accumulation, and long-term persistence of radiation-induced in vivo somatic mutations at this locus in erythroid marrow progenitor cells. Initial dosimetry and assay data from 5 patients yielded a linear GPA dose response of {approximately}6.5 induced variants/10{sup 6} cells/Gy which is 1/3 to 1/4 of that previously observed for Hiroshima A-bomb survivors and individuals exposed at the Chernobyl nuclear reactor and Goiania Cs{sup 137} source accidents who predominantly received external exposures to ionizing radiation. The lower slope of the dose response observed in the I{sup 131} treated patients may reflect a reduced biological effectiveness of this exposure due to differences in the energy spectra of the {gamma} radiation, internal versus external exposure, and/or protracted versus acute dose rate effects. Ongoing studies of I{sup 131} treated patients are designed to define the shape of the low dose response and limit of sensitivity of the GPA assay; parameters that are required for the application of the assay as a quantitative cumulative radiation biodosimeter in medical, occupational, and accidental exposure settings. This biodosimetric analysis of patients receiving very similar marrow exposures will also permit an assessment of the inter-individual variability in biological response to ionizing radiation.
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 115113
- Report Number(s):
- CONF-9503160-; ISSN 0893-6692; CNN: Grant 1 RO1 OH03276; TRN: 95:023005
- Journal Information:
- Environmental and Molecular Mutagenesis, Vol. 25, Issue Suppl.25; Conference: 26. annual Environmental Mutagen Society meeting, St. Louis, MO (United States), 12-16 Mar 1995; Other Information: PBD: 1995
- Country of Publication:
- United States
- Language:
- English
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