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Title: Combined hairpin-antisense compositions and methods for modulating expression

Abstract

A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

Inventors:
;
Publication Date:
Research Org.:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1150002
Patent Number(s):
8,796,442
Application Number:
12/052,658
Assignee:
Brookhaven Science Associates, LLC (Upton, NY) BNL
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Shanklin, John, and Nguyen, Tam. Combined hairpin-antisense compositions and methods for modulating expression. United States: N. p., 2014. Web.
Shanklin, John, & Nguyen, Tam. Combined hairpin-antisense compositions and methods for modulating expression. United States.
Shanklin, John, and Nguyen, Tam. Tue . "Combined hairpin-antisense compositions and methods for modulating expression". United States. doi:. https://www.osti.gov/servlets/purl/1150002.
@article{osti_1150002,
title = {Combined hairpin-antisense compositions and methods for modulating expression},
author = {Shanklin, John and Nguyen, Tam},
abstractNote = {A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 05 00:00:00 EDT 2014},
month = {Tue Aug 05 00:00:00 EDT 2014}
}

Patent:

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Works referenced in this record:

Inhibition of fatty acid synthase (FAS) suppresses HER2/neu (erbB-2) oncogene overexpression in cancer cells
journal, July 2004

  • Menendez, J. A.; Vellon, L.; Mehmi, I.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 29, p. 10715-10720
  • DOI: 10.1073/pnas.0403390101

Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana
journal, April 2000

  • Chuang, C.-F.; Meyerowitz, E. M.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 9, p. 4985-4990
  • DOI: 10.1073/pnas.060034297

Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
journal, May 2001

  • Elbashir, Sayda M.; Harborth, Jens; Lendeckel, Winfried
  • Nature, Vol. 411, Issue 6836, p. 494-498
  • DOI: 10.1038/35078107

Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA
journal, November 1998

  • Waterhouse, P. M.; Graham, M. W.; Wang, M.-B.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 23, p. 13959-13964
  • DOI: 10.1073/pnas.95.23.13959

Modulating seed β-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil
journal, March 2007

  • Pidkowich, M. S.; Nguyen, H. T.; Heilmann, I.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 11, p. 4742-4747
  • DOI: 10.1073/pnas.0611141104

Functional Anatomy of a dsRNA Trigger: Differential Requirement for the Two Trigger Strands in RNA Interference
journal, November 2000


Evidence that siRNAs Function as Guides, Not Primers, in the Drosophila and Human RNAi Pathways
journal, September 2002