skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Extracellular secretion of recombinant proteins

Abstract

Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.

Inventors:
;
Publication Date:
Research Org.:
National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1149687
Patent Number(s):
8,785,159
Application Number:
12/950,442
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
DOE Contract Number:  
AC36-08G028308
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Nov 19
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Linger, Jeffrey G., and Darzins, Aldis. Extracellular secretion of recombinant proteins. United States: N. p., 2014. Web.
Linger, Jeffrey G., & Darzins, Aldis. Extracellular secretion of recombinant proteins. United States.
Linger, Jeffrey G., and Darzins, Aldis. 2014. "Extracellular secretion of recombinant proteins". United States. https://www.osti.gov/servlets/purl/1149687.
@article{osti_1149687,
title = {Extracellular secretion of recombinant proteins},
author = {Linger, Jeffrey G. and Darzins, Aldis},
abstractNote = {Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.},
doi = {},
url = {https://www.osti.gov/biblio/1149687}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 22 00:00:00 EDT 2014},
month = {Tue Jul 22 00:00:00 EDT 2014}
}

Works referenced in this record:

Recombinant zymomonas for pentose fermentation
patent, May 1996


Gene coding for the E1 endoglucanase
patent, July 1996


Thermal tolerant cellulase from Acidothermus cellulolyticus
patent, June 2006


Heterologous Expression and Extracellular Secretion of Cellulolytic Enzymes by Zymomonas mobilis
journal, August 2010


What next for human gene therapy?
journal, June 2003


Transfer and expression of a Bacillus licheniformis ?-amylase gene in Zymomonas mobilis
journal, February 1990


Transfer and Expression of an Erwinia chrysanthemi Cellulase Gene in Zymomonas mobilis
journal, April 1989


The Bacterial Twin-Arginine Translocation Pathway
journal, October 2006


Characterization of multiple promoters and transcript stability in the sacB–sacC gene cluster in Zymomonas mobilis
journal, May 2009


Complete Cellulase System in the Marine Bacterium Saccharophagus degradans Strain 2-40T
journal, May 2006


Ethanol production from cellulosic materials by genetically engineered Zymomonas mobilis
journal, February 2005


Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995