Human protein tyrosine phosphatase 1C (PTPN6) gene structure: Alternate promoter usage and exon skipping general multiple transcripts
- Biotechnology Research Institute, Quebec (Canada)
PTPN6, a protein tyrosine phosphatase with two Src homology-2 domains, is expressed predominantly in cells of the hematopoietic lineage. In addition, cDNAs encoding isoforms of the enzyme differing from the latter at their N-termini have been detected in normal and transformed epithelial cells. To understand further the regulation and expression of the PTPN6 gene, the authors have isolated and characterized overlapping human genomic {lambda} clones containing the entire locus. The gene, located on chromosome 12, consists of 17 exons spanning 17 kb of DNA. Three nonhematopoietic PTPN6 transcripts were identified in a variety of cell lines and were shown to be transcribed from a common promoter. Alternate splicing and exon skipping result in transcripts encoding the two SH2 domains and minor transcripts from which part of or the entire N-terminal SH2 domain coding sequences are missing. The hematopoietic form of PTPN6 transcript is initiated at a downstream promotor separated by 7 kb from the first. This promoter is active exclusively in cells of the hematopoietic lineage. Characterization of the 5` ends of the PTPN6 mRNAs by RT-PCR and analysis of the flanking genomic sequences identified putative initiation sites within the two promoters. 44 refs., 5 figs., 1 tab.
- OSTI ID:
- 114899
- Journal Information:
- Genomics, Vol. 27, Issue 1; Other Information: PBD: 1 May 1995
- Country of Publication:
- United States
- Language:
- English
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