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Title: The genes for the {alpha}-type HC3 (PMSA2) and {beta}-type HC5 (PMSB1) subunits of human proteasomes map to chromosomes 6q27 and 7p12-p13 by fluorescence in situ hybridization

Abstract

The authors have determined the locations of the genes for the two subunits, HC3 and HC5, by fluorescence in situ hybridization (FISH). Chromosome spreads were obtained from phytohemagglutinin-stimulated blood lymphocytes of a healthy donor after thymidine synchronization and bromodeoxyuridine incorporation by the method of Takahashi et al. Genomic DNA fragments of HC3 (4.3 kb, including exons 3, 4, and 5) and HC5 (7.5 kb including exons 1 and 2) (11) were labeled with biotin-16-dUTP by nick-translation. In situ hybridization was performed according to Lichter et al. in the presence of COT-1 DNA as a competitor. Hybridized probe was detected with FITC-conjugated avidin without further signal amplification. Comparison of the fluorescence signals and the banding patterns of the chromosomes indicated that the HC3 and HC5 genes were located on chromosome band 6q27 and 7p12-p13, respectively.

Authors:
; ;  [1]
  1. Mie Univ., Tsu (Japan) [and others
Publication Date:
OSTI Identifier:
114853
Resource Type:
Journal Article
Resource Relation:
Journal Name: Genomics; Journal Volume: 27; Journal Issue: 2; Other Information: PBD: 20 May 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PEPTIDE HYDROLASES; GENES; HUMAN CHROMOSOME 6; GENETIC MAPPING; HUMAN CHROMOSOME 7; DNA HYBRIDIZATION

Citation Formats

Okumura, Katsuzumi, Nogami, Masahiro, and Taguchi, Hiroshi. The genes for the {alpha}-type HC3 (PMSA2) and {beta}-type HC5 (PMSB1) subunits of human proteasomes map to chromosomes 6q27 and 7p12-p13 by fluorescence in situ hybridization. United States: N. p., 1995. Web. doi:10.1006/geno.1995.1062.
Okumura, Katsuzumi, Nogami, Masahiro, & Taguchi, Hiroshi. The genes for the {alpha}-type HC3 (PMSA2) and {beta}-type HC5 (PMSB1) subunits of human proteasomes map to chromosomes 6q27 and 7p12-p13 by fluorescence in situ hybridization. United States. doi:10.1006/geno.1995.1062.
Okumura, Katsuzumi, Nogami, Masahiro, and Taguchi, Hiroshi. Sat . "The genes for the {alpha}-type HC3 (PMSA2) and {beta}-type HC5 (PMSB1) subunits of human proteasomes map to chromosomes 6q27 and 7p12-p13 by fluorescence in situ hybridization". United States. doi:10.1006/geno.1995.1062.
@article{osti_114853,
title = {The genes for the {alpha}-type HC3 (PMSA2) and {beta}-type HC5 (PMSB1) subunits of human proteasomes map to chromosomes 6q27 and 7p12-p13 by fluorescence in situ hybridization},
author = {Okumura, Katsuzumi and Nogami, Masahiro and Taguchi, Hiroshi},
abstractNote = {The authors have determined the locations of the genes for the two subunits, HC3 and HC5, by fluorescence in situ hybridization (FISH). Chromosome spreads were obtained from phytohemagglutinin-stimulated blood lymphocytes of a healthy donor after thymidine synchronization and bromodeoxyuridine incorporation by the method of Takahashi et al. Genomic DNA fragments of HC3 (4.3 kb, including exons 3, 4, and 5) and HC5 (7.5 kb including exons 1 and 2) (11) were labeled with biotin-16-dUTP by nick-translation. In situ hybridization was performed according to Lichter et al. in the presence of COT-1 DNA as a competitor. Hybridized probe was detected with FITC-conjugated avidin without further signal amplification. Comparison of the fluorescence signals and the banding patterns of the chromosomes indicated that the HC3 and HC5 genes were located on chromosome band 6q27 and 7p12-p13, respectively.},
doi = {10.1006/geno.1995.1062},
journal = {Genomics},
number = 2,
volume = 27,
place = {United States},
year = {Sat May 20 00:00:00 EDT 1995},
month = {Sat May 20 00:00:00 EDT 1995}
}