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Title: BioBriefcase.

Abstract

Abstract not provided.

Authors:
; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1147967
Report Number(s):
SAND2007-3137C
522984
DOE Contract Number:
AC04-94AL85000
Resource Type:
Conference
Resource Relation:
Conference: Proposed for presentation at the 6th DHS Conference on Chemical and Biological Technologies held June 5-8, 2007 in Madison, WI.
Country of Publication:
United States
Language:
English

Citation Formats

Fruetel, Julia A., Renzi, Ronald F., and Bailey, Christopher. BioBriefcase.. United States: N. p., 2007. Web.
Fruetel, Julia A., Renzi, Ronald F., & Bailey, Christopher. BioBriefcase.. United States.
Fruetel, Julia A., Renzi, Ronald F., and Bailey, Christopher. Tue . "BioBriefcase.". United States. doi:. https://www.osti.gov/servlets/purl/1147967.
@article{osti_1147967,
title = {BioBriefcase.},
author = {Fruetel, Julia A. and Renzi, Ronald F. and Bailey, Christopher},
abstractNote = {Abstract not provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 01 00:00:00 EDT 2007},
month = {Tue May 01 00:00:00 EDT 2007}
}

Conference:
Other availability
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  • The BioBriefcase is an integrated briefcase-sized aerosol collection and analysis system for autonomous monitoring of the environment, which is currently being jointly developed by Lawrence Livermore and Sandia National Laboratories. This poster presents results from the polymerase chain reaction (PCR) module of the system. The DNA must be purified after exiting the aerosol collector to prevent inhibition of the enzymatic reaction. Traditional solid-phase extraction results in a large loss of sample. In this flow-through system, we perform sample purification, concentration and amplification in one reactor, which minimizes the loss of material. The sample from the aerosol collector is mixed withmore » a denaturation solution prior to flowing through a capillary packed with silica beads. The DNA adheres to the silica beads allowing the environmental contaminants to be flushed to waste while effectively concentrating the DNA on the silica matrix. The adhered DNA is amplified while on the surface of the silica beads, resulting in a lower limit of detection than an equivalent eluted sample. Thus, this system is beneficial since more DNA is available for amplification, less reagents are utilized, and contamination risks are reduced.« less
  • No abstract prepared.
  • Abstract not provided.
  • A system for sampling air and collecting particles potentially including bioagents entrained in the air for detection. The system comprises collecting a sample of the air with the particles entrained in the air, directing the sample to a receiving surface, directing a liquid to the receiving surface thereby producing a liquid surface, wherein the particles potentially including bioagents become captured in the liquid, and heating the liquid wherein the particles potentially including bioagents become heated to lysis the bioagents.