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Title: Enhanced Biomass-Bioenergy Conversion through Enzyme Engineering.


Abstract not provided.

; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
Report Number(s):
DOE Contract Number:
Resource Type:
Resource Relation:
Conference: Proposed for presentation at the Biochemical Engineering XV Engineering Biology from Biomolecules to Complex Systems held July 14 - March 19, 2007 in Quebec City, QC, Canada.
Country of Publication:
United States

Citation Formats

Simmons, Blake, Roe, Diana C., Volponi, Joanne V., Faulon, Jean-Loup M., and Buffleben, George M. Enhanced Biomass-Bioenergy Conversion through Enzyme Engineering.. United States: N. p., 2007. Web.
Simmons, Blake, Roe, Diana C., Volponi, Joanne V., Faulon, Jean-Loup M., & Buffleben, George M. Enhanced Biomass-Bioenergy Conversion through Enzyme Engineering.. United States.
Simmons, Blake, Roe, Diana C., Volponi, Joanne V., Faulon, Jean-Loup M., and Buffleben, George M. Thu . "Enhanced Biomass-Bioenergy Conversion through Enzyme Engineering.". United States. doi:.
title = {Enhanced Biomass-Bioenergy Conversion through Enzyme Engineering.},
author = {Simmons, Blake and Roe, Diana C. and Volponi, Joanne V. and Faulon, Jean-Loup M. and Buffleben, George M.},
abstractNote = {Abstract not provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 2007},
month = {Thu Mar 01 00:00:00 EST 2007}

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  • The cellulosic fraction of biomass can be converted to ethanol, a promising alternative renewable fuel, using the simultaneous saccharification and fermentation (SSF) process. The SSF integrates the enzymatic hydrolysis of cellulose to glucose with the fermentation of glucose to ethanol. Ethanol productivity and yield depend on the characteristics of the biomass, the quality of the cellulase enzyme complex, and the behavior of the fermentative organism. The effects of key engineering factors on SSF performance were studied using real biomass substrate and realistic process conditions. The experimental results indicate that corn steep liquor can replace expensive lab media for the SSF.more » Saccharomyces cerevisiae D{sub 5}A outperforms other leading organisms in terms of both high ethanol productivity and minimal by-product formation. Using that organism, the SSF operational conditions (pH and temperature) were varied in order to optimize the synergism between the cellulase enzyme complex and the fermentative yeast. The SSF performance was optimal at 40{degrees}C and initial pH of 4.0--5.0. Higher temperatures resulted in a significant decrease in cell viability and productivity.« less
  • Abstract not provided.
  • The complexity of biomass systems acts as an additional constraint in developing and designing feasible and sustainable technologies and processes for the large-scale production of fuels and other non-food products from biological feedstocks. The object of this paper is to present and discuss a new modelling approach, and the corresponding PC-aided tools ({open_quotes}Biomass Toolkit{close_quotes}), making possible the quantitative description of such complex processes and production systems by performing mass and energy balances, as well as other related calculations (of inputs, pollutants, CO{sub 2} effects, other side-effects, etc). This work is based on recent developments in the emerging field of biosystemsmore » modelling; biosystems dynamics are described with the help of a minimum number of parameters, having clear physical and/or technical significance; default values of all those parameters are provided to assist the non-expert user. Two types of biosystems are considered, i.e., based on (a) the cultivation of typical energy crops; and (b) the integrated utilization of agricultural residues as biofuels. {open_quotes}Toolkit{close_quotes} is a user-friendly, interactive computer programme, written in Borland C++ language, and operating under Windows PC environment.« less
  • The conference proceedings consist of two volumes of papers detailing numerous issues related to biomass energy production and use. An author and keyword index are provided in the proceedings. A total of 143 papers were selected for the database. Papers were selected from the following areas from Volume 1: feedstock production, harvest, storage, and delivery; the DOE biomass power program; technical, economic, and policy barriers and incentives; new developments in biomass combustion; advancements in biomass gasification; liquid fuels production and use; and case studies of bioenergy projects. From Volume 2, subtopics selected included: bioenergy systems for distributed generation; assessment andmore » use of biomass wastes; non-technical barriers to bioenergy implementation; improving commercial viability through integrated systems; and anaerobic digestion.« less