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Title: Detecting Plasmon Resonance Energy Transfer with Differential Interference Contrast Microscopy

Abstract

Gold nanoparticles are ideal probes for studying intracellular environments and energy transfer mechanisms due to their plasmonic properties. Plasmon resonance energy transfer (PRET) relies on a plasmonic nanoparticle to donate energy to a nearby resonant acceptor molecule, a process which can be observed due to the plasmonic quenching of the donor nanoparticle. In this study, a gold nanosphere was used as the plasmonic donor, while the metalloprotein cytochrome c was used as the acceptor molecule. Differential interference contrast (DIC) microscopy allows for simultaneous monitoring of complex environments and noble metal nanoparticles in real time. Using DIC and specially designed microfluidic channels, we were able to monitor PRET at the single gold particle level and observe the reversibility of PRET upon the introduction of phosphate-buffered saline to the channel. In an additional experiment, single gold particles were internalized by HeLa cells and were subsequently observed undergoing PRET as the cell hosts underwent morphological changes brought about by ethanol-induced apoptosis.

Authors:
 [1];  [1];  [1];  [1]
  1. Ames Lab., Ames, IA (United States); Iowa State Univ., Ames, IA (United States)
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1134615
Report Number(s):
IS-J 8235
Journal ID: ISSN 0003-2700
DOE Contract Number:  
AC02-07CH11358
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 86; Journal Issue: 2; Journal ID: ISSN 0003-2700
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; Biochemical Methods

Citation Formats

Augspurger, Ashley E., Stender, Anthony S., Han, Rui, and Fang, Ning. Detecting Plasmon Resonance Energy Transfer with Differential Interference Contrast Microscopy. United States: N. p., 2013. Web. doi:10.1021/ac403347e.
Augspurger, Ashley E., Stender, Anthony S., Han, Rui, & Fang, Ning. Detecting Plasmon Resonance Energy Transfer with Differential Interference Contrast Microscopy. United States. https://doi.org/10.1021/ac403347e
Augspurger, Ashley E., Stender, Anthony S., Han, Rui, and Fang, Ning. Mon . "Detecting Plasmon Resonance Energy Transfer with Differential Interference Contrast Microscopy". United States. https://doi.org/10.1021/ac403347e.
@article{osti_1134615,
title = {Detecting Plasmon Resonance Energy Transfer with Differential Interference Contrast Microscopy},
author = {Augspurger, Ashley E. and Stender, Anthony S. and Han, Rui and Fang, Ning},
abstractNote = {Gold nanoparticles are ideal probes for studying intracellular environments and energy transfer mechanisms due to their plasmonic properties. Plasmon resonance energy transfer (PRET) relies on a plasmonic nanoparticle to donate energy to a nearby resonant acceptor molecule, a process which can be observed due to the plasmonic quenching of the donor nanoparticle. In this study, a gold nanosphere was used as the plasmonic donor, while the metalloprotein cytochrome c was used as the acceptor molecule. Differential interference contrast (DIC) microscopy allows for simultaneous monitoring of complex environments and noble metal nanoparticles in real time. Using DIC and specially designed microfluidic channels, we were able to monitor PRET at the single gold particle level and observe the reversibility of PRET upon the introduction of phosphate-buffered saline to the channel. In an additional experiment, single gold particles were internalized by HeLa cells and were subsequently observed undergoing PRET as the cell hosts underwent morphological changes brought about by ethanol-induced apoptosis.},
doi = {10.1021/ac403347e},
url = {https://www.osti.gov/biblio/1134615}, journal = {Analytical Chemistry},
issn = {0003-2700},
number = 2,
volume = 86,
place = {United States},
year = {2013},
month = {12}
}