Decreased abundance of type III secretion system-inducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae

Anderson, Jeffrey C.; Wan, Ying; Kim, Young-Mo; Pasa-Tolic, Ljiljana; Metz, Thomas O.; Peck, Scott C.

doi:10.1073/pnas.1403248111

Title: Decreased abundance of type III secretion system-inducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae

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Abstract

Many phytopathogenic bacteria use a type III secretion system (T3SS) to inject defense-suppressing effector proteins into host cells. Genes encoding the T3SS are induced at the start of infection, yet host signals that initiate T3SS gene expression are poorly understood. Here we identify several plant-derived metabolites that induce the T3SS in the bacterial pathogen Pseudomonas syringae pv tomato DC3000. In addition, we report that mkp1 (mapk phosphatase 1), an Arabidopsis mutant that is more resistant to bacterial infection, produces decreased levels of these T3SS-inducing metabolites. Consistent with the observed decrease in these metabolites, T3SS effector delivery by DC3000 was impaired in mkp1. Addition of the bioactive metabolites to the mkp1-DC3000 interaction fully restored T3SS effector delivery and suppressed enhanced resistance in mkp1. Together, these results demonstrate that DC3000 perceives multiple signals derived from plants to initiate their virulence program, and reveal a new layer of molecular communication between plants and these pathogenic bacteria.

Authors:: Anderson, Jeffrey C.; Wan, Ying; Kim, Young-Mo; Pasa-Tolic, Ljiljana; Metz, Thomas O.; Peck, Scott C.

Publication Date:: Mon Apr 21 00:00:00 EDT 2014

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1134508

Report Number(s):: PNNL-SA-97264
47395; KP1704020

DOE Contract Number:: AC05-76RL01830

Resource Type:: Journal Article

Journal Name:: Proceedings of the National Academy of Sciences of the United States of America, 111(18):6846-6851

Additional Journal Information:: Journal Name: Proceedings of the National Academy of Sciences of the United States of America, 111(18):6846-6851

Country of Publication:: United States

Language:: English

Subject:: Environmental Molecular Sciences Laboratory

Citation Formats


                    Anderson, Jeffrey C., Wan, Ying, Kim, Young-Mo, Pasa-Tolic, Ljiljana, Metz, Thomas O., and Peck, Scott C. Decreased abundance of type III secretion system-inducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae.  United States: N. p., 2014. 
        Web.  doi:10.1073/pnas.1403248111.

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                    Anderson, Jeffrey C., Wan, Ying, Kim, Young-Mo, Pasa-Tolic, Ljiljana, Metz, Thomas O., & Peck, Scott C. Decreased abundance of type III secretion system-inducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae.  United States.  https://doi.org/10.1073/pnas.1403248111

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                    Anderson, Jeffrey C., Wan, Ying, Kim, Young-Mo, Pasa-Tolic, Ljiljana, Metz, Thomas O., and Peck, Scott C. 2014.  
        "Decreased abundance of type III secretion system-inducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae".  United States.  https://doi.org/10.1073/pnas.1403248111.

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@article{osti_1134508,

  title        = {Decreased abundance of type III secretion system-inducing signals in Arabidopsis mkp1 enhances resistance against Pseudomonas syringae},

  author       = {Anderson, Jeffrey C. and Wan, Ying and Kim, Young-Mo and Pasa-Tolic, Ljiljana and Metz, Thomas O. and Peck, Scott C.},

  abstractNote = {Many phytopathogenic bacteria use a type III secretion system (T3SS) to inject defense-suppressing effector proteins into host cells. Genes encoding the T3SS are induced at the start of infection, yet host signals that initiate T3SS gene expression are poorly understood. Here we identify several plant-derived metabolites that induce the T3SS in the bacterial pathogen Pseudomonas syringae pv tomato DC3000. In addition, we report that mkp1 (mapk phosphatase 1), an Arabidopsis mutant that is more resistant to bacterial infection, produces decreased levels of these T3SS-inducing metabolites. Consistent with the observed decrease in these metabolites, T3SS effector delivery by DC3000 was impaired in mkp1. Addition of the bioactive metabolites to the mkp1-DC3000 interaction fully restored T3SS effector delivery and suppressed enhanced resistance in mkp1. Together, these results demonstrate that DC3000 perceives multiple signals derived from plants to initiate their virulence program, and reveal a new layer of molecular communication between plants and these pathogenic bacteria.},

  doi          = {10.1073/pnas.1403248111},

  url          = {https://www.osti.gov/biblio/1134508},
  journal      = {Proceedings of the National Academy of Sciences of the United States of America, 111(18):6846-6851},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon Apr 21 00:00:00 EDT 2014},

  month        = {Mon Apr 21 00:00:00 EDT 2014}

}

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