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Title: Characterization and analysis of the molecular weight of lignin for biorefining studies

Abstract

The molecular weight of lignin is a fundamental property that infl uences the recalcitrance of biomass and the valorization of lignin. The determination of the molecular weight of lignin in native biomass is dependent on the bioresources used and the isolation and purifi cation procedures employed. The three most commonly employed isolation methods are milled wood lignin (MWL), cellulolytic enzyme lignin (CEL), and enzymatic mild acidolysis lignin (EMAL). Common characterization techniques for determining the molecular weight of lignin will be addressed, with an emphasis on gel permeation chromatography (GPC). This review also examines the mechanisms behind several biological, physical, and chemical pre-treatments and their impact on the molecular weight of lignin. The number average molecular weight (Mn), weight average molecular weight (Mw) and polydispersity index (D) all vary in magnitude depending on the biomass source, pre-treatment conditions, and isolation method. Additionally, there is a growing body of literature that supports changes in the molecular weight of lignin in response to genetic modifi cations in the lignin biosynthetic pathways. This review summarizes different procedures for obtaining the molecular weight of lignin that have been used in recent years and highlight future opportunities for applications of lignin.

Authors:
 [1];  [1];  [1];  [2];  [3]
  1. Georgia Institute of Technology, Atlanta GA USA
  2. Oak Ridge National Laboratory, Oak Ridge TN USA
  3. BioEnergy Science Center, Institute of Paper Science and Technology, School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta GA USA
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1134191
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Biofuels, Bioproducts & Biorefining
Additional Journal Information:
Journal Volume: 8; Journal Issue: 6; Journal ID: ISSN 1932-104X
Publisher:
Wiley
Country of Publication:
United States
Language:
English

Citation Formats

Tolbert, Allison, Akinosho, Hannah, Khunsupat, Ratayakorn, Naskar, Amit K., and Ragauskas, Arthur J. Characterization and analysis of the molecular weight of lignin for biorefining studies. United States: N. p., 2014. Web. doi:10.1002/bbb.1500.
Tolbert, Allison, Akinosho, Hannah, Khunsupat, Ratayakorn, Naskar, Amit K., & Ragauskas, Arthur J. Characterization and analysis of the molecular weight of lignin for biorefining studies. United States. doi:10.1002/bbb.1500.
Tolbert, Allison, Akinosho, Hannah, Khunsupat, Ratayakorn, Naskar, Amit K., and Ragauskas, Arthur J. Wed . "Characterization and analysis of the molecular weight of lignin for biorefining studies". United States. doi:10.1002/bbb.1500.
@article{osti_1134191,
title = {Characterization and analysis of the molecular weight of lignin for biorefining studies},
author = {Tolbert, Allison and Akinosho, Hannah and Khunsupat, Ratayakorn and Naskar, Amit K. and Ragauskas, Arthur J.},
abstractNote = {The molecular weight of lignin is a fundamental property that infl uences the recalcitrance of biomass and the valorization of lignin. The determination of the molecular weight of lignin in native biomass is dependent on the bioresources used and the isolation and purifi cation procedures employed. The three most commonly employed isolation methods are milled wood lignin (MWL), cellulolytic enzyme lignin (CEL), and enzymatic mild acidolysis lignin (EMAL). Common characterization techniques for determining the molecular weight of lignin will be addressed, with an emphasis on gel permeation chromatography (GPC). This review also examines the mechanisms behind several biological, physical, and chemical pre-treatments and their impact on the molecular weight of lignin. The number average molecular weight (Mn), weight average molecular weight (Mw) and polydispersity index (D) all vary in magnitude depending on the biomass source, pre-treatment conditions, and isolation method. Additionally, there is a growing body of literature that supports changes in the molecular weight of lignin in response to genetic modifi cations in the lignin biosynthetic pathways. This review summarizes different procedures for obtaining the molecular weight of lignin that have been used in recent years and highlight future opportunities for applications of lignin.},
doi = {10.1002/bbb.1500},
journal = {Biofuels, Bioproducts & Biorefining},
issn = {1932-104X},
number = 6,
volume = 8,
place = {United States},
year = {2014},
month = {6}
}

Works referenced in this record:

Electrospray Ionization Mass Spectrometry as a Tool for Lignins Molecular Weight and Structural Characterisation
journal, September 1999

  • Evtuguin, D. V.; Domingues, P.; Amado, F. L.
  • Holzforschung, Vol. 53, Issue 5
  • DOI: 10.1515/HF.1999.086

Solution-State Nuclear Magnetic Resonance Study of the Similarities between Milled Wood Lignin and Cellulolytic Enzyme Lignin
journal, February 2004

  • Holtman, Kevin M.; Chang, Hou-min; Kadla, John F.
  • Journal of Agricultural and Food Chemistry, Vol. 52, Issue 4
  • DOI: 10.1021/jf035084k

Assessing the molecular structure basis for biomass recalcitrance during dilute acid and hydrothermal pretreatments
journal, January 2013


Lignin – from natural adsorbent to activated carbon: A review
journal, September 2007


Comparative Evaluation of Three Lignin Isolation Protocols for Various Wood Species
journal, December 2006

  • Guerra, Anderson; Filpponen, Ilari; Lucia, Lucian A.
  • Journal of Agricultural and Food Chemistry, Vol. 54, Issue 26
  • DOI: 10.1021/jf062433c

Comparative study of lignins isolated by alkali and ultrasound-assisted alkali extractions from wheat straw
journal, March 2002


Organosolv Ethanol Lignin from Hybrid Poplar as a Radical Scavenger:  Relationship between Lignin Structure, Extraction Conditions, and Antioxidant Activity
journal, August 2006

  • Pan, Xuejun; Kadla, John F.; Ehara, Katsunobu
  • Journal of Agricultural and Food Chemistry, Vol. 54, Issue 16
  • DOI: 10.1021/jf0605392

Molecular weight distribution and structural characteristics of polymers obtained from acid soluble lignin treated by oxidative enzymes
journal, March 2001


Cellulosic biorefineries—unleashing lignin opportunities
journal, December 2010

  • Sannigrahi, Poulomi; Pu, Yunqiao; Ragauskas, Arthur
  • Current Opinion in Environmental Sustainability, Vol. 2, Issue 5-6
  • DOI: 10.1016/j.cosust.2010.09.004

Quantitative 13 C NMR Characterization of Milled Wood Lignins Isolated by Different Milling Techniques
journal, April 2006

  • Holtman, Kevin M.; Chang, Hou‐min; Jameel, Hasan
  • Journal of Wood Chemistry and Technology, Vol. 26, Issue 1
  • DOI: 10.1080/02773810600582152

Structural Characterization and Comparison of Switchgrass Ball-milled Lignin Before and After Dilute Acid Pretreatment
journal, August 2009

  • Samuel, Reichel; Pu, Yunqiao; Raman, Babu
  • Applied Biochemistry and Biotechnology, Vol. 162, Issue 1
  • DOI: 10.1007/s12010-009-8749-y

A two-stage pretreatment approach to maximise sugar yield and enhance reactive lignin recovery from poplar wood chips
journal, February 2013


Biomass Characterization: Recent Progress in Understanding Biomass Recalcitrance
journal, August 2012

  • Foston, Marcus; Ragauskas, Arthur J.
  • Industrial Biotechnology, Vol. 8, Issue 4
  • DOI: 10.1089/ind.2012.0015

Effect of replacing polyol by organosolv and kraft lignin on the property and structure of rigid polyurethane foam
journal, January 2013


Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification
journal, July 2010


Characterization of Miscanthus giganteus Lignin Isolated by Ethanol Organosolv Process under Reflux Condition
journal, August 2012

  • Bauer, Stefan; Sorek, Hagit; Mitchell, Valerie D.
  • Journal of Agricultural and Food Chemistry, Vol. 60, Issue 33
  • DOI: 10.1021/jf302409d

Screening of a fungus capable of powerful and selective delignification on wheat straw
journal, November 2008


Sequential extractions and structural characterization of lignin with ethanol and alkali from bamboo (Neosinocalamus affinis)
journal, May 2012


Analysis of the accuracy of determining average molecular weights of narrow polydispersity polymers by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
journal, April 1998

  • Zhu, Honghui; Yalcin, Talat; Li, Liang
  • Journal of the American Society for Mass Spectrometry, Vol. 9, Issue 4
  • DOI: 10.1016/S1044-0305(97)00292-4

Polymer Structure of Lignophenol II —Comparison of Molecular Morphology of Lignophenol and Conventional Lignins—
journal, June 2006


Microwave pretreatment for enzymatic saccharification of sweet sorghum bagasse
journal, April 2012


Improving aging resistance and mechanical properties of waterborne polyurethanes modified by lignin amines
journal, May 2013

  • Liu, Jun; Liu, Hai-Feng; Deng, Li
  • Journal of Applied Polymer Science, Vol. 130, Issue 3
  • DOI: 10.1002/app.39267

The Path Forward for Biofuels and Biomaterials
journal, January 2006

  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

Isolation and characterization of lignins from Eucalyptus grandis Hill ex Maiden and Eucalyptus globulus Labill. by enzymatic mild acidolysis (EMAL)
journal, January 2008

  • Guerra, Anderson; Lucia, Lucian A.; Argyropoulos, Dimitris S.
  • Holzforschung, Vol. 62, Issue 1
  • DOI: 10.1515/HF.2008.004

Improving wheat gluten materials properties by Kraft lignin addition
journal, January 2012

  • Kunanopparat, Thiranan; Menut, Paul; Morel, Marie-Hélène
  • Journal of Applied Polymer Science, Vol. 125, Issue 2
  • DOI: 10.1002/app.35345

The absolute molecular weight distribution of hydroxypropylated lignins
journal, September 1990

  • Siochi, Emilie J.; Ward, Thomas C.; Haney, Max A.
  • Macromolecules, Vol. 23, Issue 5
  • DOI: 10.1021/ma00207a029

Chemistry of lignin-based materials
journal, September 2013


The chemistry of several novel bioconversion lignins
journal, September 1983

  • Glasser, Wolfgang G.; Barnett, Charlotte A.; Muller, Peter C.
  • Journal of Agricultural and Food Chemistry, Vol. 31, Issue 5
  • DOI: 10.1021/jf00119a001

Monodisperse lignin fractions as standards in size-exclusion analysis
journal, May 2009

  • Botaro, Vagner Roberto; Curvelo, Antonio Aprígio da Silva
  • Journal of Chromatography A, Vol. 1216, Issue 18
  • DOI: 10.1016/j.chroma.2009.02.039

Molecular weight distribution of wood components extracted from Pinus taeda biotreated by Ceriporiopsis subvermispora
journal, July 2003


Poplar as a feedstock for biofuels: A review of compositional characteristics
journal, March 2010

  • Sannigrahi, Poulomi; Ragauskas, Arthur J.; Tuskan, Gerald A.
  • Biofuels, Bioproducts and Biorefining, Vol. 4, Issue 2
  • DOI: 10.1002/bbb.206

Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
journal, January 2007

  • Masai, Eiji; Katayama, Yoshihiro; Fukuda, Masao
  • Bioscience, Biotechnology, and Biochemistry, Vol. 71, Issue 1
  • DOI: 10.1271/bbb.60437

Characterization of lignin during oxidative and hydrothermal pre-treatment processes of wheat straw and corn stover
journal, May 2010


The emerging role for bacteria in lignin degradation and bio-product formation
journal, June 2011

  • Bugg, Timothy DH; Ahmad, Mark; Hardiman, Elizabeth M.
  • Current Opinion in Biotechnology, Vol. 22, Issue 3
  • DOI: 10.1016/j.copbio.2010.10.009

Association behaviour of lignins and lignin model compounds studied by multidetector size-exclusion chromatography
journal, December 2003


Size-exclusion chromatography of lignin as ion-pair complex
journal, March 1997


Chemical transformations of Populus trichocarpa during dilute acid pretreatment
journal, January 2012

  • Cao, Shilin; Pu, Yunqiao; Studer, Michael
  • RSC Advances, Vol. 2, Issue 29
  • DOI: 10.1039/c2ra22045h

Bioconversion of Beetle-Killed Lodgepole Pine Using SPORL: Process Scale-up Design, Lignin Coproduct, and High Solids Fermentation without Detoxification
journal, October 2013

  • Zhou, Haifeng; Zhu, J. Y.; Luo, Xiaolin
  • Industrial & Engineering Chemistry Research, Vol. 52, Issue 45
  • DOI: 10.1021/ie402873y

Molecular Weight Distributions and Linkages in Lignocellulosic Materials Derivatized from Ionic Liquid Media
journal, February 2011

  • Zoia, Luca; King, Alistair W. T.; Argyropoulos, Dimitris S.
  • Journal of Agricultural and Food Chemistry, Vol. 59, Issue 3
  • DOI: 10.1021/jf103615e

Accurate and Reproducible Determination of Lignin Molar Mass by Acetobromination
journal, March 2012

  • Asikkala, Janne; Tamminen, Tarja; Argyropoulos, Dimitris S.
  • Journal of Agricultural and Food Chemistry, Vol. 60, Issue 36
  • DOI: 10.1021/jf303003d

Mass Spectrometry of Polymers and Polymer Surfaces
journal, February 2001


Insights into lignin primary structure and deconstruction from Arabidopsis thaliana COMT (caffeic acid O-methyl transferase) mutant Atomt1
journal, January 2010

  • Moinuddin, Syed G. A.; Jourdes, Michaël; Laskar, Dhrubojyoti D.
  • Organic & Biomolecular Chemistry, Vol. 8, Issue 17
  • DOI: 10.1039/c004817h

Woody biomass pretreatment for cellulosic ethanol production: Technology and energy consumption evaluation☆
journal, July 2010


Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Comparative Studies on Cellulolytic Enzyme Lignin and Milled Wood Lignin of Sweetgum and Spruce
journal, January 1975


Lignin-based Carbon Fibers: Effect of Synthetic Polymer Blending on Fiber Properties
journal, April 2005


Determination of lignin by size exclusion chromatography using multi angle laser light scattering
journal, May 2006


Effects of process severity on the chemical structure of Miscanthus ethanol organosolv lignin
journal, June 2010


Chemical aspects of kraft pulping
journal, January 1980


Lignin. VI. Molecular Weights of Lignin Sulfonates by Light Scattering 1
journal, July 1955

  • Moacanin, Jovan; Felicetta, Vincent F.; Haller, Wolfgang
  • Journal of the American Chemical Society, Vol. 77, Issue 13
  • DOI: 10.1021/ja01618a011

Conversion of Lignin with Supercritical Water−Phenol Mixtures
journal, July 2003

  • Saisu, Motofumi; Sato, Takafumi; Watanabe, Masaru
  • Energy & Fuels, Vol. 17, Issue 4
  • DOI: 10.1021/ef0202844

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


Polymerization of Kraft lignin via ultrasonication for high-molecular-weight applications
journal, November 2013


A structural model of softwood lignin
journal, January 1980


Chemistry of softwood lignin degradation byStreptomyces viridosporus
journal, March 1982

  • Crawford, Don L.; Barder, Michael J.; Pometto, Anthony L.
  • Archives of Microbiology, Vol. 131, Issue 2
  • DOI: 10.1007/BF01053996

Enhanced characteristics of genetically modified switchgrass (Panicum virgatum L.) for high biofuel production
journal, January 2013

  • Shen, Hui; Poovaiah, Charleson R.; Ziebell, Angela
  • Biotechnology for Biofuels, Vol. 6, Issue 1
  • DOI: 10.1186/1754-6834-6-71

Mass spectrometry in the biosynthetic and structural investigation of lignins
journal, January 2004

  • Reale, Samantha; Di Tullio, Alessandra; Spreti, Nicoletta
  • Mass Spectrometry Reviews, Vol. 23, Issue 2
  • DOI: 10.1002/mas.10072

Kraft Lignin Chain Extension Chemistry via Propargylation, Oxidative Coupling, and Claisen Rearrangement
journal, September 2013

  • Sen, Sanghamitra; Sadeghifar, Hasan; Argyropoulos, Dimitris S.
  • Biomacromolecules, Vol. 14, Issue 10
  • DOI: 10.1021/bm4010172

Lignin Formation in Plants. The Dilemma of Linkage Specificity
journal, August 2001


Chemical and thermal characteristics of lignins isolated from Siam weed stem by acetic acid and formic acid delignification
journal, November 2010


Characterization of lignin using multi-angle laser light scattering and atomic force microscopy
journal, January 2006


Structural features affecting biomass enzymatic digestibility
journal, June 2008


Unmasking the structural features and property of lignin from bamboo
journal, March 2013


Quantitative Structures and Thermal Properties of Birch Lignins after Ionic Liquid Pretreatment
journal, January 2013

  • Wen, Jia-Long; Sun, Shao-Long; Xue, Bai-Liang
  • Journal of Agricultural and Food Chemistry, Vol. 61, Issue 3
  • DOI: 10.1021/jf3051939

Investigation of the chemical modifications of beech wood lignin during heat treatment
journal, September 2010


Changes in lignocellulosic supramolecular and ultrastructure during dilute acid pretreatment of Populus and switchgrass
journal, December 2010


Studies on the Effect of Ball Milling on Lignin Structure Using a Modified DFRC Method
journal, January 2002

  • Ikeda, Tsutomu; Holtman, Kevin; Kadla, John F.
  • Journal of Agricultural and Food Chemistry, Vol. 50, Issue 1
  • DOI: 10.1021/jf010870f

Morphological characteristics and composition of lipophilic extractives and lignin in Brazilian woods from different eucalypt hybrids
journal, March 2012


High-performance size exclusion chromatography of low-molecular-weight lignins and model compounds
journal, January 1983


Fractionation of wheat straw by atmospheric acetic acid process
journal, July 2005


Concentration and purification of lignin in hardwood kraft pulping liquor by ultrafiltration and nanofiltration
journal, November 2008

  • Jönsson, Ann-Sofi; Nordin, Anna-Karin; Wallberg, Ola
  • Chemical Engineering Research and Design, Vol. 86, Issue 11
  • DOI: 10.1016/j.cherd.2008.06.003

The Wood-Based Biorefinery: A Source of Carbon Fiber?
journal, December 2010


Polydispersity index and molecular weight distributions of polymers
journal, November 1996


On the propensity of lignin to associate: A size exclusion chromatography study with lignin derivatives isolated from different plant species
journal, October 2007


Molar mass determination of lignins by size-exclusion chromatography: towards standardisation of the method
journal, June 2007

  • Baumberger, Stéphanie; Abaecherli, Alfred; Fasching, Mario
  • Holzforschung, Vol. 61, Issue 4
  • DOI: 10.1515/HF.2007.074

Analyzing cellulose degree of polymerization and its relevancy to cellulosic ethanol
journal, January 2011

  • Hallac, Bassem B.; Ragauskas, Arthur J.
  • Biofuels, Bioproducts and Biorefining, Vol. 5, Issue 2
  • DOI: 10.1002/bbb.269

Enzymatic conversion of lignocellulose into fermentable sugars: challenges and opportunities
journal, October 2007

  • Jørgensen, Henning; Kristensen, Jan Bach; Felby, Claus
  • Biofuels, Bioproducts and Biorefining, Vol. 1, Issue 2
  • DOI: 10.1002/bbb.4

Improved calibration procedure for gel permeation chromatography of lignins
journal, July 1974


Isolation and characterization of herbaceous lignins for applications in biomaterials
journal, January 2013


New insights into the molecular architecture of hardwood lignins by chemical degradative methods
journal, March 1995

  • Lapierre, Catherine; Pollet, Brigitte; Rolando, Christian
  • Research on Chemical Intermediates, Vol. 21, Issue 3-5, p. 397-412
  • DOI: 10.1007/BF03052266

Dilute-Acid Hydrolysis of Lignocellulosic Biomass
book, January 1999

  • Lee, Y. Y.; Iyer, Prashant; Torget, R. W.
  • Recent Progress in Bioconversion of Lignocellulosics, p. 93-115
  • DOI: 10.1007/3-540-49194-5_5

Structural Characterization of Switchgrass Lignin after Ethanol Organosolv Pretreatment
journal, December 2011

  • Hu, Gang; Cateto, Carolina; Pu, Yunqiao
  • Energy & Fuels, Vol. 26, Issue 1
  • DOI: 10.1021/ef201477p

Microwave-enhanced extraction of lignin from birch in formic acid: Structural characterization and antioxidant activity study
journal, December 2012


Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Microwave-assisted extraction of lignin from triticale straw: Optimization and microwave effects
journal, January 2012


Lignin and Lignin-Carbohydrate Complexes
journal, September 1957

  • Bjorkman, Anders.
  • Industrial & Engineering Chemistry, Vol. 49, Issue 9
  • DOI: 10.1021/ie50573a040

Toward a Better Understanding of the Lignin Isolation Process from Wood
journal, August 2006

  • Guerra, Anderson; Filpponen, Ilari; Lucia, Lucian A.
  • Journal of Agricultural and Food Chemistry, Vol. 54, Issue 16
  • DOI: 10.1021/jf060722v

Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations
journal, September 2006


Quantitative evaluation of milling effects on lignin structure during the isolation process of milled wood lignin
journal, February 2005


Increase in 4-Coumaryl Alcohol Units during Lignification in Alfalfa ( Medicago sativa ) Alters the Extractability and Molecular Weight of Lignin
journal, October 2010

  • Ziebell, Angela; Gracom, Kristen; Katahira, Rui
  • Journal of Biological Chemistry, Vol. 285, Issue 50
  • DOI: 10.1074/jbc.M110.137315

Lignin Structural Modifications Resulting from Ethanol Organosolv Treatment of Loblolly Pine
journal, January 2010

  • Sannigrahi, Poulomi; Ragauskas, Arthur J.; Miller, Stephen J.
  • Energy & Fuels, Vol. 24, Issue 1, p. 683-689
  • DOI: 10.1021/ef900845t

Methanol Fractionation of Softwood Kraft Lignin: Impact on the Lignin Properties
journal, November 2013

  • Saito, Tomonori; Perkins, Joshua H.; Vautard, Frederic
  • ChemSusChem, Vol. 7, Issue 1
  • DOI: 10.1002/cssc.201300509

Isolation and Structural Characterization of Lignin from Cotton Stalk Treated in an Ammonia Hydrothermal System
journal, November 2012

  • Kang, Sumin; Xiao, Lingping; Meng, Lingyan
  • International Journal of Molecular Sciences, Vol. 13, Issue 12
  • DOI: 10.3390/ijms131115209

Review of current and future softwood kraft lignin process chemistry
journal, September 2004


Value-adding to cellulosic ethanol: Lignin polymers
journal, March 2011

  • Doherty, William O. S.; Mousavioun, Payam; Fellows, Christopher M.
  • Industrial Crops and Products, Vol. 33, Issue 2
  • DOI: 10.1016/j.indcrop.2010.10.022

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the analysis of polydienes
journal, December 1997

  • Yalcin, Talat; Schriemer, David C.; Li, Liang
  • Journal of the American Society for Mass Spectrometry, Vol. 8, Issue 12
  • DOI: 10.1016/S1044-0305(97)00192-X

Evaluation of Biological Pretreatment of Rubberwood with White Rot Fungi for Enzymatic Hydrolysis
journal, May 2013

  • Nazarpour, Forough; Abdullah, Dzulkefly; Abdullah, Norhafizah
  • Materials, Vol. 6, Issue 5
  • DOI: 10.3390/ma6052059

Characterization of Lignins Isolated with Alkaline Ethanol from the Hydrothermal Pretreated Tamarix ramosissima
journal, October 2012


Development of novel assays for lignin degradation: comparative analysis of bacterial and fungal lignin degraders
journal, January 2010

  • Ahmad, Mark; Taylor, Charles R.; Pink, David
  • Molecular BioSystems, Vol. 6, Issue 5
  • DOI: 10.1039/b908966g

Characterisation of lignosulphonates and kraft lignin by hydrophobic interaction chromatography
journal, April 2006

  • Ekeberg, Dag; Gretland, Kristin Sandersen; Gustafsson, Jonas
  • Analytica Chimica Acta, Vol. 565, Issue 1
  • DOI: 10.1016/j.aca.2006.02.008

Extraction of lignin from lignocellulose at atmospheric pressure using alkylbenzenesulfonate ionic liquid
journal, January 2009

  • Tan, Suzie S. Y.; MacFarlane, Douglas R.; Upfal, Jonathan
  • Green Chemistry, Vol. 11, Issue 3
  • DOI: 10.1039/b815310h

Improved lignin properties and reactivity by modifications in the autohydrolysis process of aspen wood
journal, March 2008


Chemical synthesis of β-O-4 type artificial lignin
journal, January 2006

  • Kishimoto, Takao; Uraki, Yasumitsu; Ubukata, Makoto
  • Organic & Biomolecular Chemistry, Vol. 4, Issue 7
  • DOI: 10.1039/b518005h

Isolation of high-purity residual lignins from eucalypt paper pulps by cellulase and proteinase treatments followed by solvent extraction
journal, August 2004


Chemical and Physicochemical Pretreatment of Lignocellulosic Biomass: A Review
journal, January 2011

  • Brodeur, Gary; Yau, Elizabeth; Badal, Kimberly
  • Enzyme Research, Vol. 2011
  • DOI: 10.4061/2011/787532

Aqueous Gel Permeation Chromatographic Methods for Technical Lignins
journal, August 2000


Characteristics of degraded lignins obtained from steam exploded wheat straw
journal, November 2004


Disruption of sugarcane bagasse lignocellulosic structure by means of dilute sulfuric acid pretreatment with microwave-assisted heating
journal, August 2011


Lignin in straw of herbaceous crops
journal, November 2008


Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production
journal, April 2009

  • Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J.
  • Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729
  • DOI: 10.1021/ie801542g

Liquid fuels, hydrogen and chemicals from lignin A critical review
journal, May 2013

  • Azadi, Pooya; Inderwildi, Oliver R.; Farnood, Ramin
  • Renewable and Sustainable Energy Reviews, Vol. 21, p. 506-523
  • DOI: 10.1016/j.rser.2012.12.022

The Effects on Lignin Structure of Overexpression of Ferulate 5-Hydroxylase in Hybrid Poplar 1
journal, April 2009

  • Stewart, Jaclyn J.; Akiyama, Takuya; Chapple, Clint
  • Plant Physiology, Vol. 150, Issue 2
  • DOI: 10.1104/pp.109.137059

Using Transgenic Poplars to Elucidate the Relationship between the Structure and the Thermal Properties of Lignins
journal, April 2002

  • Baumberger, Stéphanie; Dole, Patrice; Lapierre, Catherine
  • Journal of Agricultural and Food Chemistry, Vol. 50, Issue 8
  • DOI: 10.1021/jf0113530

Biomass pretreatment: Fundamentals toward application
journal, November 2011


Biomass Characterization of Buddleja davidii: A Potential Feedstock for Biofuel Production
journal, February 2009

  • Hallac, Bassem B.; Sannigrahi, Poulomi; Pu, Yunqiao
  • Journal of Agricultural and Food Chemistry, Vol. 57, Issue 4
  • DOI: 10.1021/jf8030277

Characterization of milled wood lignin and ethanol organosolv lignin from miscanthus
journal, October 2009


Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption
journal, April 2013


Microwave-assisted dissolution and delignification of wood in 1-ethyl-3-methylimidazolium acetate
journal, May 2013


Size-exclusion chromatography of technical lignins in dimethyl sulfoxide/water and dimethylacetamide
journal, January 2006


Analytical protocols for characterisation of sulphur-free lignin
journal, May 2004


Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine
journal, April 2009