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Title: Microstructured block copolymer surfaces for control of microbe capture and aggregation

Abstract

The capture and arrangement of surface-associated microbes is influenced by biochemical and physical properties of the substrate. In this report, we develop lectin-functionalized substrates containing patterned, three-dimensional polymeric structures of varied shapes and densities and use these to investigate the effects of topology and spatial confinement on lectin-mediated microbe capture. Films of poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA) were patterned on silicon surfaces into line or square grid patterns with 5 m wide features and varied edge spacing. The patterned films had three-dimensional geometries with 900 nm film thickness. After surface functionalization with wheat germ agglutinin, the size of Pseudomonas fluorescens aggregates captured was dependent on the pattern dimensions. Line patterns with edge spacing of 5 m or less led to the capture of individual microbes with minimal formation of aggregates, while grid patterns with the same spacing also captured individual microbes with further reduction in aggregation. Both geometries allowed for increases in aggregate size distribution with increased in edge spacing. These engineered surfaces combine spatial confinement with affinity-based microbe capture based on exopolysaccharide content to control the degree of microbe aggregation, and can also be used as a platform to investigate intercellular interactions and biofilm formation in microbial populations of controlled sizes.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1]
  1. ORNL
  2. University of Tennessee, Knoxville (UTK)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Center for Nanophase Materials Sciences (CNMS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1134167
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Biosensors
Additional Journal Information:
Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2079--6374
Country of Publication:
United States
Language:
English
Subject:
lectins; affinity-based capture; cell aggregation; block copolymers; biofilms

Citation Formats

Hansen, Ryan R, Shubert, Katherine R, Morrell, Jennifer L., Lokitz, Bradley S, Doktycz, Mitchel John, and Retterer, Scott T. Microstructured block copolymer surfaces for control of microbe capture and aggregation. United States: N. p., 2014. Web. doi:10.3390/bios4010063.
Hansen, Ryan R, Shubert, Katherine R, Morrell, Jennifer L., Lokitz, Bradley S, Doktycz, Mitchel John, & Retterer, Scott T. Microstructured block copolymer surfaces for control of microbe capture and aggregation. United States. https://doi.org/10.3390/bios4010063
Hansen, Ryan R, Shubert, Katherine R, Morrell, Jennifer L., Lokitz, Bradley S, Doktycz, Mitchel John, and Retterer, Scott T. Wed . "Microstructured block copolymer surfaces for control of microbe capture and aggregation". United States. https://doi.org/10.3390/bios4010063.
@article{osti_1134167,
title = {Microstructured block copolymer surfaces for control of microbe capture and aggregation},
author = {Hansen, Ryan R and Shubert, Katherine R and Morrell, Jennifer L. and Lokitz, Bradley S and Doktycz, Mitchel John and Retterer, Scott T},
abstractNote = {The capture and arrangement of surface-associated microbes is influenced by biochemical and physical properties of the substrate. In this report, we develop lectin-functionalized substrates containing patterned, three-dimensional polymeric structures of varied shapes and densities and use these to investigate the effects of topology and spatial confinement on lectin-mediated microbe capture. Films of poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA) were patterned on silicon surfaces into line or square grid patterns with 5 m wide features and varied edge spacing. The patterned films had three-dimensional geometries with 900 nm film thickness. After surface functionalization with wheat germ agglutinin, the size of Pseudomonas fluorescens aggregates captured was dependent on the pattern dimensions. Line patterns with edge spacing of 5 m or less led to the capture of individual microbes with minimal formation of aggregates, while grid patterns with the same spacing also captured individual microbes with further reduction in aggregation. Both geometries allowed for increases in aggregate size distribution with increased in edge spacing. These engineered surfaces combine spatial confinement with affinity-based microbe capture based on exopolysaccharide content to control the degree of microbe aggregation, and can also be used as a platform to investigate intercellular interactions and biofilm formation in microbial populations of controlled sizes.},
doi = {10.3390/bios4010063},
url = {https://www.osti.gov/biblio/1134167}, journal = {Biosensors},
issn = {2079--6374},
number = 1,
volume = 4,
place = {United States},
year = {2014},
month = {1}
}