Methods for targetted mutagenesis in gram-positive bacteria

Yang, Yunfeng

Title: Methods for targetted mutagenesis in gram-positive bacteria

Patent · Tue May 27 00:00:00 EDT 2014

OSTI ID:1132802

Yang, Yunfeng

The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

View Patent

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-000R22725

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 8,735,160

Application Number:: 12/233,806

OSTI ID:: 1132802

Resource Relation:: Patent File Date: 2008 Sep 19

Country of Publication:: United States

Language:: English

References (8)

Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824 Green, E. M.; Boynton, Z. L.; Harris, L. M. Microbiology, Vol. 142, Issue 8 https://doi.org/10.1099/13500872-142-8-2079	journal	August 1996
Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824 Harris, L. M.; Welker, N. E.; Papoutsakis, E. T. Journal of Bacteriology, Vol. 184, Issue 13 https://doi.org/10.1128/JB.184.13.3586-3597.2002	journal	July 2002
Gene transfer to Clostridium cellulolyticum ATCC 35319 Jennert, Katrin C. B.; Young, Danielle I.; Young, Michael Microbiology, Vol. 146, Issue 12 https://doi.org/10.1099/00221287-146-12-3071	journal	December 2000
Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K Kolter, Roberto; Inuzuka, Manabu; Helinski, Donald R. Cell, Vol. 15, Issue 4, p. 1199-1208 https://doi.org/10.1016/0092-8674(78)90046-6	journal	December 1978
Studies on cellulase production by Clostridium thermocellum Garcia-Martinez, D. V.; Shinmyo, A.; Madia, A. European Journal of Applied Microbiology and Biotechnology, Vol. 9, Issue 3 https://doi.org/10.1007/BF00504485	journal	January 1980
Use of antisense RNA to modify the composition of cellulosomes produced by Clostridium cellulolyticum: asRNA downregulation of a C. cellulolyticum cellulase Perret, Stéphanie; Maamar, Hédia; Bélaich, Jean-Pierre Molecular Microbiology, Vol. 51, Issue 2 https://doi.org/10.1046/j.1365-2958.2003.03860.x	journal	January 2004
Electrotransformation studies in Clostridium cellulolyticum Tardif, C.; Maamar, H.; Balfin, M. Journal of Industrial Microbiology and Biotechnology, Vol. 27, Issue 5 https://doi.org/10.1038/sj.jim.7000081	journal	November 2001
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002

Similar Records

Transformation of gram positive bacteria by sonoporation

Patent · Tue Mar 11 00:00:00 EDT 2014 · OSTI ID:1132802

Yang, Yunfeng; Li, Yongchao

New Transposon Tools Tailored for Metabolic Engineering of Gram-Negative Microbial Cell Factories

Journal Article · Tue Oct 28 00:00:00 EDT 2014 · Frontiers in Bioengineering and Biotechnology · OSTI ID:1132802

Martínez-García, Esteban; Aparicio, Tomás; Lorenzo, Víctor de

Ethanol production in Gram-positive microbes

Patent · Tue Jan 09 00:00:00 EST 1996 · OSTI ID:1132802

Ingram, L O; Barbosa-Alleyne, M D.F.

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: Methods for targetted mutagenesis in gram-positive bacteria

Citation Formats

References (8)

Similar Records

Related Subjects