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Title: Methods for targetted mutagenesis in gram-positive bacteria

Abstract

The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

Inventors:
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1132802
Patent Number(s):
8,735,160
Application Number:
12/233,806
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
DOE Contract Number:  
AC05-000R22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Sep 19
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Yang, Yunfeng. Methods for targetted mutagenesis in gram-positive bacteria. United States: N. p., 2014. Web.
Yang, Yunfeng. Methods for targetted mutagenesis in gram-positive bacteria. United States.
Yang, Yunfeng. Tue . "Methods for targetted mutagenesis in gram-positive bacteria". United States. https://www.osti.gov/servlets/purl/1132802.
@article{osti_1132802,
title = {Methods for targetted mutagenesis in gram-positive bacteria},
author = {Yang, Yunfeng},
abstractNote = {The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.},
doi = {},
url = {https://www.osti.gov/biblio/1132802}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {5}
}

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Works referenced in this record:

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Gene transfer to Clostridium cellulolyticum ATCC 35319
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Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K
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Studies on cellulase production by Clostridium thermocellum
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Electrotransformation studies in Clostridium cellulolyticum
journal, November 2001


Microbial Cellulose Utilization: Fundamentals and Biotechnology
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