Methods for targetted mutagenesis in gram-positive bacteria

Yang, Yunfeng

Methods for targetted mutagenesis in gram-positive bacteria

Patent · 27 May 2014

OSTI ID:1132802

Yang, Yunfeng

The present invention provides a method of targeted mutagenesis in Gram-positive bacteria. In particular, the present invention provides a method that effectively integrates a suicide integrative vector into a target gene in the chromosome of a Gram-positive bacterium, resulting in inactivation of the target gene.

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 8,735,160

Application Number:: 12/233,806

OSTI ID:: 1132802

Country of Publication:: United States

Language:: English

References (9)

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Cellulosomes from Clostridium thermocellum Lamed, Raphael; Bayer, Edward A. Methods in Enzymology, Vol. 160, p. 472-482 https://doi.org/10.1016/0076-6879(88)60157-1	book	December 1987
Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K Kolter, Roberto; Inuzuka, Manabu; Helinski, Donald R. Cell, Vol. 15, Issue 4, p. 1199-1208 https://doi.org/10.1016/0092-8674(78)90046-6	journal	December 1978
Electrotransformation studies in Clostridium cellulolyticum Tardif, C.; Maamar, H.; Balfin, M. Journal of Industrial Microbiology and Biotechnology, Vol. 27, Issue 5 https://doi.org/10.1038/sj.jim.7000081	journal	November 2001
Use of antisense RNA to modify the composition of cellulosomes produced by Clostridium cellulolyticum: asRNA downregulation of a C. cellulolyticum cellulase Perret, Stéphanie; Maamar, Hédia; Bélaich, Jean-Pierre Molecular Microbiology, Vol. 51, Issue 2 https://doi.org/10.1046/j.1365-2958.2003.03860.x	journal	January 2004
Gene transfer to Clostridium cellulolyticum ATCC 35319 Jennert, Katrin C. B.; Young, Danielle I.; Young, Michael Microbiology, Vol. 146, Issue 12 https://doi.org/10.1099/00221287-146-12-3071	journal	December 2000
Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824 Green, E. M.; Boynton, Z. L.; Harris, L. M. Microbiology, Vol. 142, Issue 8 https://doi.org/10.1099/13500872-142-8-2079	journal	August 1996
Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824 Harris, L. M.; Welker, N. E.; Papoutsakis, E. T. Journal of Bacteriology, Vol. 184, Issue 13 https://doi.org/10.1128/JB.184.13.3586-3597.2002	journal	July 2002
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002

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Methods for targetted mutagenesis in gram-positive bacteria

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