skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods of saccharification of polysaccharides in plants

Abstract

Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional .beta.-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.

Inventors:
;
Publication Date:
Research Org.:
Applied Biotechnology Institute, Inc., San Luis Obispo, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1130067
Patent Number(s):
8,709,761
Application Number:
13/025,659
Assignee:
Applied Biotechnology Institute, Inc. (San Luis Obispo, CA)
DOE Contract Number:  
FG36-08GO88025
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Feb 11
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Howard, John, and Fake, Gina. Methods of saccharification of polysaccharides in plants. United States: N. p., 2014. Web.
Howard, John, & Fake, Gina. Methods of saccharification of polysaccharides in plants. United States.
Howard, John, and Fake, Gina. Tue . "Methods of saccharification of polysaccharides in plants". United States. https://www.osti.gov/servlets/purl/1130067.
@article{osti_1130067,
title = {Methods of saccharification of polysaccharides in plants},
author = {Howard, John and Fake, Gina},
abstractNote = {Saccharification of polysaccharides of plants is provided, where release of fermentable sugars from cellulose is obtained by adding plant tissue composition. Production of glucose is obtained without the need to add additional .beta.-glucosidase. Adding plant tissue composition to a process using a cellulose degrading composition to degrade cellulose results in an increase in the production of fermentable sugars compared to a process in which plant tissue composition is not added. Using plant tissue composition in a process using a cellulose degrading enzyme composition to degrade cellulose results in decrease in the amount of cellulose degrading enzyme composition or exogenously applied cellulase required to produce fermentable sugars.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {4}
}

Patent:

Save / Share:

Works referenced in this record:

Synergism between corn stover protein and cellulase
journal, October 2007


Synergism between hydrophobic proteins of corn stover and cellulase in lignocellulose hydrolysis
journal, January 2010


Changes in Composition during Development and Maturation of Maize Seeds
journal, September 1965

  • Ingle, John; Beitz, D.; Hageman, R. H.
  • Plant Physiology, Vol. 40, Issue 5
  • DOI: 10.1104/pp.40.5.835

Production of recombinant proteins in transgenic plants: Practical considerations
journal, December 1997


Expression of Trichoderma reesei Exo-Cellobiohydrolase I in Transgenic Tobacco Leaves and Calli
journal, January 1999

  • Dai, Ziyu; Hooker, Brian S.; Quesenberry, Ryan D.
  • Applied Biochemistry and Biotechnology, Vol. 79, Issue 1-3
  • DOI: 10.1385/ABAB:79:1-3:689

Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants
journal, October 2003

  • Woodard, Susan L.; Mayor, Jocelyne M.; Bailey, Michele R.
  • Biotechnology and Applied Biochemistry, Vol. 38, Issue 2, p. 123-130
  • DOI: 10.1042/BA20030026

The production of recombinant proteins in transgenic barley grains
journal, February 2000

  • Horvath, H.; Huang, J.; Wong, O.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 4
  • DOI: 10.1073/pnas.030527497

Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose
journal, January 2007

  • Gusakov, Alexander V.; Salanovich, Tatyana N.; Antonov, Alexey I.
  • Biotechnology and Bioengineering, Vol. 97, Issue 5
  • DOI: 10.1002/bit.21329

Expression of a Ruminococcus albus cellulase gene in tobacco suspension cells
journal, January 1996

  • Kawazu, Tetsu; Ohta, Tsutomu; Ito, Kazuya
  • Journal of Fermentation and Bioengineering, Vol. 82, Issue 3, p. 205-209
  • DOI: 10.1016/0922-338X(96)88809-X

Expression of a bacterial endoglucanase gene in tobacco increases digestibility of its cell wall fibers
journal, January 1999


Enhanced conversion of plant biomass into glucose using transgenic rice-produced endoglucanase for cellulosic ethanol
journal, January 2007


A novel method for evaluating the release of fermentable sugars from cellulosic biomass
journal, October 2010


Synergistic Activity of Plant Extracts with Microbial Cellulases for the Release of Free Sugars
journal, August 2011


Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627
journal, November 2007


Expression of thermostable microbial cellulases in the chloroplasts of nicotine-free tobacco
journal, September 2007


Biochemical characterization of a maize stover β-exoglucanase and its use in lignocellulose conversion
journal, August 2010


Plant genetic engineering to improve biomass characteristics for biofuels
journal, June 2006


Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol
journal, June 2008

  • Sticklen, Mariam B.
  • Nature Reviews Genetics, Vol. 9, Issue 6
  • DOI: 10.1038/nrg2336