skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity

Abstract

A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1129019
Patent Number(s):
8,685,743
Application Number:
12/772,118
Assignee:
The Regents of the University of California (Oakland, CA) LBNL
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Apr 30
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Zhang, Xiang, Ellman, Jonathan A, Chen, Fanqing Frank, Su, Kai-Hang, Wei, Qi-Huo, and Sun, Cheng. Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity. United States: N. p., 2014. Web.
Zhang, Xiang, Ellman, Jonathan A, Chen, Fanqing Frank, Su, Kai-Hang, Wei, Qi-Huo, & Sun, Cheng. Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity. United States.
Zhang, Xiang, Ellman, Jonathan A, Chen, Fanqing Frank, Su, Kai-Hang, Wei, Qi-Huo, and Sun, Cheng. Tue . "Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity". United States. doi:. https://www.osti.gov/servlets/purl/1129019.
@article{osti_1129019,
title = {Real-time, single-step bioassay using nanoplasmonic resonator with ultra-high sensitivity},
author = {Zhang, Xiang and Ellman, Jonathan A and Chen, Fanqing Frank and Su, Kai-Hang and Wei, Qi-Huo and Sun, Cheng},
abstractNote = {A nanoplasmonic resonator (NPR) comprising a metallic nanodisk with alternating shielding layer(s), having a tagged biomolecule conjugated or tethered to the surface of the nanoplasmonic resonator for highly sensitive measurement of enzymatic activity. NPRs enhance Raman signals in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real-time, at picomolar sensitivity levels. Experiments on extracellular fluid (ECF) from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background in real-time single-step detection in very small sample volumes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 01 00:00:00 EDT 2014},
month = {Tue Apr 01 00:00:00 EDT 2014}
}

Patent:

Save / Share:

Works referenced in this record:

New biochip technology for label-free detection of pathogens and their toxins
journal, May 2003

  • Grow, Ann E.; Wood, Laurie L.; Claycomb, Johanna L.
  • Journal of Microbiological Methods, Vol. 53, Issue 2, p. 221-233
  • DOI: 10.1016/S0167-7012(03)00026-5

Surface-enhanced Raman scattering on tunable plasmonic nanoparticle substrates
journal, December 2004

  • Jackson, J. B.; Halas, N. J.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 52, p. 17930-17935
  • DOI: 10.1073/pnas.0408319102

Periodically structured metallic substrates for SERS
journal, August 1998


Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman Scattering
journal, February 1997


Characterization and application of Raman labels for confocal Raman microspectroscopic detection of cellular proteins in single cells
journal, November 2003

  • Nithipatikom, Kasem; McCoy, Michael J.; Hawi, Sharon R.
  • Analytical Biochemistry, Vol. 322, Issue 2, p. 198-207
  • DOI: 10.1016/j.ab.2003.07.020

Catalytic hydroxyl/amine dyads within serine proteases
journal, January 1997


On the size of the active site in proteases. I. Papain
journal, April 1967

  • Schechter, Israel; Berger, Arieh
  • Biochemical and Biophysical Research Communications, Vol. 27, Issue 2, p. 157-162
  • DOI: 10.1016/S0006-291X(67)80055-X