ADS genes for reducing saturated fatty acid levels in seed oils

Heilmann, Ingo H; Shanklin, John

Title: ADS genes for reducing saturated fatty acid levels in seed oils

Patent · Tue Mar 18 00:00:00 EDT 2014

OSTI ID:1127126

Heilmann, Ingo H; Shanklin, John

The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.

View Patent

Cite

Export

Save

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-98CH10886

Assignee:: BNL

Patent Number(s):: 8,674,176

Application Number:: 12/686,975

OSTI ID:: 1127126

Country of Publication:: United States

Language:: English

References (154)

Process for amplifying, detecting, and/or-cloning nucleic acid sequences Mullis, Kary B.; Erlich, Henry A.; Arnheim, Norman https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683195 US Patent Document 4,683,195	patent	July 1987
Process for amplifying nucleic acid sequences Mullis, Kary B. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4683202 US Patent Document 4,683,202	patent	July 1987
Process for the incorporation of foreign dna into the genome of dicotyledonous plants Schilperoort, Robbert A.; Hoekema, Andreas; Hooykaas, Paul https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4940838 US Patent Document 4,940,838	patent	July 1990
Method for transporting substances into living cells and tissues and apparatus therefor Sanford, John C.; Wolf, Edward D.; Allen, Nelson K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4945050 US Patent Document 4,945,050	patent	July 1990
Single polypeptide chain binding molecules Ladner, Robert Charles; Bird, Robert; Hardman, Karl D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4946778 US Patent Document 4,946,778	patent	August 1990
Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme Mullis, Kary B.; Erlich, Henry A.; Gelfand, David H. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/4965188 US Patent Document 4,965,188	patent	October 1990
Recombinant DNA: transformed microorganisms, plant cells and plants: a process for introducing an inducible property in plants, and a process for producing a polypeptide or protein by means of plants or plant cells Bol, John Ferdinand; Cornelissen, Bernardus Johannes Clemens; van Kan, Johannes Arnoldus Laurentius https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5057422 US Patent Document 5,057,422	patent	October 1991
Generation and selection of novel DNA-binding proteins and polypeptides Ladner, Robert Charles; Guterman, Sonia; Kent, Rachel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5096815 US Patent Document 5,096,815	patent	March 1992
Transfer vector Ahlquist, Paul G. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5173410 US Patent Document 5,173,410	patent	December 1992
Plant proteins, promoters, coding sequences and use Comai, Luca; Koning, Ann J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5187267 US Patent Document 5,187,267	patent	February 1993
Generation and selection of novel DNA-binding proteins and polypeptides Ladner, Robert Charles; Guterman, Sonia; Kent, Rachel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5198346 US Patent Document 5,198,346	patent	March 1993
Directed evolution of novel binding proteins Ladner, Robert Charles; Guterman, Sonia; Roberts, Bruce L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5223409 US Patent Document 5,223,409	patent	June 1993
Chimeric genes for transforming plant cells using viral promoters Fraley, Robert T.; Horsch, Robert B.; Rogers, Stephen G. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5352605 US Patent Document 5,352,605	patent	October 1994
Method for stably transforming plastids of multicellular plants Maliga, Pal; Maliga, Zora S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5451513 US Patent Document 5,451,513	patent	September 1995
RNA transformation vector Ahlquist, Paul G.; French, Roy C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5500360 US Patent Document 5,500,360	patent	March 1996
Process for the site-directed integration of DNA into the genome of plants Offringa, Remko; de Groot, Marcellus Johannes Augustinus; Hooykaas, Paul https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5501967 US Patent Document 5,501,967	patent	March 1996
Enhanced expression in a plant plastid McBride, Kevin E.; Stalker, David M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5545817 US Patent Document 5,545,817	patent	August 1996
Expression of Bacillus thuringiensis cry proteins in plant plastids McBride, Kevin E.; Maliga, Pal https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5545818 US Patent Document 5,545,818	patent	August 1996
Gas driven gene delivery instrument McCabe, Dennis E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5584807 US Patent Document 5,584,807	patent	December 1996
Peptide library and screening method Schatz, Peter J.; Cull, Millard; Miller, Jeff F. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5733731 US Patent Document 5,733,731	patent	March 1998
Methods for generating polynucleotides having desired characteristics by iterative selection and recombination Stemmer, Willem P. C.; Crameri, Andreas https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5811238 US Patent Document 5,811,238	patent	September 1998
Method for soybean transformation and regeneration Hinchee, Maud A.; Connor-Ward, Dannette https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5824877 US Patent Document 5,824,877	patent	October 1998
DNA mutagenesis by random fragmentation and reassembly Stemmer, Willem P. C.; Crameri, Andreas https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5830721 US Patent Document 5,830,721	patent	November 1998
RNA transformation vector Ahlquist, Paul G.; French, Roy C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5846795 US Patent Document 5,846,795	patent	December 1998
Recombinant plant viral nucleic acids Donson, Jon; Dawson, William O.; Granthan, George L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5866785 US Patent Document 5,866,785	patent	February 1999
Viral amplification of recombinant messenger RNA in transgenic plants Turpen, Thomas H. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5965794 US Patent Document 5,965,794	patent	October 1999
Production of peptides in plants as viral coat protein fusions Turpen, Thomas H.; Reinl, Stephen; Grill, Laurence K. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5977438 US Patent Document 5,977,438	patent	November 1999
Methods and compositions for regulated transcription and expression of heterologous genes Knauf, Vic C.; Kridl, Jean C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5981839 US Patent Document 5,981,839	patent	November 1999
Methods for agrobacterium-mediated transformation Zhao, Zuo-Yu; Gu, Weining; Cai, Tishu https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5981840 US Patent Document 5,981,840	patent	November 1999
Regeneration of plants containing genetically engineered T-DNA Barton, Kenneth; Binns, Andrew Norton; Chilton, Mary-Dell https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6051757 US Patent Document 6,051,757	patent	April 2000
Canola oil having increased oleic acid and decreased linolenic acid content DeBonte, Lorin R.; Hitz, William D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6063947 US Patent Document 6,063,947	patent	May 2000
Plant stearoyl-ACP desaturases genes Thompson, Gregory A.; Knauf, Vic C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6117677 US Patent Document 6,117,677	patent	September 2000
Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants Lightner, Jonathan; Okuley, John Joseph; Hitz, William D. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6372965 US Patent Document 6,372,965	patent	April 2002
ADS genes for reducing saturated fatty acid levels in seed oils Heilmann, Ingo H.; Shanklin, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7655833 US Patent Document 7,655,833	patent	February 2010
Selective modification of plant fatty acids Yao, Kening; Taylor, David C.; Friesen, Laurie https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030056246 US Patent Application 09/866061; 20030056246	patent-application	March 2003
ADS genes for reducing saturated fatty acid levels in seed oils Heilmann, Ingo H.; Shanklin, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050039234 US Patent Application 10/857765; 20050039234	patent-application	February 2005
Two FAD3 Desaturase Genes Control the Level of Linolenic Acid in Flax Seed Vrinten, Patricia; Hu, Zhiyuan; Munchinsky, Mary-Ann Plant Physiology, Vol. 139, Issue 1 https://doi.org/10.1104/pp.105.064451	journal	August 2005
Protein annotation: detective work for function prediction Doerks, T. Trends in Genetics, Vol. 14, Issue 6 https://doi.org/10.1016/S0168-9525(98)01486-3	journal	June 1998
Go hunting in sequence databases but watch out for the traps Bork, Peer; Bairoch, Amos Trends in Genetics, Vol. 12, Issue 10, p. 425-427 https://doi.org/10.1016/0168-9525(96)60040-7	journal	October 1996
Efficient functioning of plant promoters and poly(A) sites in Xenopus oocytes Ballas, Nurit; Broido, Shimshon; Soreq, Hermona Nucleic Acids Research, Vol. 17, Issue 19 https://doi.org/10.1093/nar/17.19.7891	journal	January 1989
Exploring the Hydroxylation−Dehydrogenation Connection: Novel Catalytic Activity of Castor Stearoyl-ACP Δ ⁹ Desaturase Behrouzian, Behnaz; Savile, Christopher K.; Dawson, Brian Journal of the American Chemical Society, Vol. 124, Issue 13 https://doi.org/10.1021/ja012252l	journal	April 2002
Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure. Ben-Bassat, A.; Bauer, K.; Chang, S. Y. Journal of Bacteriology, Vol. 169, Issue 2 https://doi.org/10.1128/jb.169.2.751-757.1987	journal	February 1987
A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation Bevan, Michael W.; Flavell, Richard B.; Chilton, Mary-Dell Nature, Vol. 304, Issue 5922 https://doi.org/10.1038/304184a0	journal	July 1983
A Rapid Method of Total Lipid Extraction and Purification Bligh, E. G.; Dyer, W. J. Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 1 https://doi.org/10.1139/y59-099	journal	January 1959
Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells. Blochlinger, K.; Diggelmann, H. Molecular and Cellular Biology, Vol. 4, Issue 12 https://doi.org/10.1128/MCB.4.12.2929	journal	December 1984
A bifunctional oleate 12‐hydroxylase: desaturase from Lesquerella fendleri Broun, Pierre; Boddupalli, Sekhar; Somerville, Chris The Plant Journal, Vol. 13, Issue 2 https://doi.org/10.1046/j.1365-313X.1998.00023.x	journal	January 1998
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids Broun, Pierre; Shanklin, John; Whittle, Ed Science, Vol. 282, Issue 5392, p. 1315-1317 https://doi.org/10.1126/science.282.5392.1315	journal	November 1998
Trialkylsulfonium- and Trialkylselenoniumhydroxides for the Pyrolytic Alkylation of Acidic Compounds Butte, W.; Eilers, J.; Kirsch, M. Analytical Letters, Vol. 15, Issue 10 https://doi.org/10.1080/00032718208069519	journal	January 1982
Randomization of genes by PCR mutagenesis. Cadwell, R. C.; Joyce, G. F. Genome Research, Vol. 2, Issue 1, p. 28-33 https://doi.org/10.1101/gr.2.1.28	journal	August 1992
Introns increase gene expression in cultured maize cells. Callis, J.; Fromm, M.; Walbot, V. Genes & Development, Vol. 1, Issue 10, p. 1183-1200 https://doi.org/10.1101/gad.1.10.1183	journal	December 1987
Organ-specific modulation of gene expression in transgenic plants using antisene RNA Cannon, Maura; Platz, Jerry; O'Leary, Maureen Plant Molecular Biology, Vol. 15, Issue 1 https://doi.org/10.1007/BF00017722	journal	July 1990
New RNA Polymerase from Escherichia coli infected with Bacteriophage T7 Chamberlin, Michael; McGrath, Janet; Waskell, Lucy Nature, Vol. 228, Issue 5268, p. 227-231 https://doi.org/10.1038/228227a0	journal	October 1970
Leucine Aminopeptidase RNAs, Proteins, and Activities Increase in Response to Water Deficit, Salinity, and the Wound Signals Systemin, Methyl Jasmonate, and Abscisic Acid Chao, Wun S.; Gu, Yong-Qiang; Pautot, Véronique Plant Physiology, Vol. 120, Issue 4 https://doi.org/10.1104/pp.120.4.979	journal	August 1999
Regulatory Elements That Control Transcription Activation and Unsaturated Fatty Acid-mediated Repression of the Saccharomyces cerevisiae OLE1 Gene Choi, Jae-Yeon; Stukey, Joseph; Hwang, Sue-Yun Journal of Biological Chemistry, Vol. 271, Issue 7 https://doi.org/10.1074/jbc.271.7.3581	journal	February 1996
Lipid Analysis: Isolation, Separation, Identification and Structural Analysis of Lipids Berry, Sarah Nutrition Bulletin, Vol. 29, Issue 1 https://doi.org/10.1111/j.1467-3010.2003.00361.x	journal	March 2004
Production of Transgenic Rice (Oryza Sativa L.) Plants from Agronomically Important Indica and Japonica Varieties via Electric Discharge Particle Acceleration of Exogenous DNA into Immature Zygotic Embryos Christou, Paul; Ford, Tameria L.; Kofron, Matt Bio/Technology, Vol. 9, Issue 10 https://doi.org/10.1038/nbt1091-957	journal	October 1991
Stable Transformation of Soybean Callus by DNA-Coated Gold Particles Christou, Paul; McCabe, Dennis E.; Swain, William F. Plant Physiology, Vol. 87, Issue 3 https://doi.org/10.1104/pp.87.3.671	journal	July 1988
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana Clough, Steven J.; Bent, Andrew F. The Plant Journal, Vol. 16, Issue 6, p. 735-743 https://doi.org/10.1046/j.1365-313x.1998.00343.x	journal	December 1998
Monoclonal antibodies and cancer therapy Reisfeld, Ralph A.; Sell, Stewart Journal of Cellular Biochemistry, Vol. 29, Issue S9A, p. 33-74 https://doi.org/10.1002/jcb.240290503	journal	January 1985
Molecular evolution of an arsenate detoxification pathway by DNA shuffling Crameri, Andreas; Dawes, Glenn; Rodriguez, Emilio Nature Biotechnology, Vol. 15, Issue 5 https://doi.org/10.1038/nbt0597-436	journal	May 1997
Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling Crameri, Andreas; Whitehorn, Erik A.; Tate, Emily Nature Biotechnology, Vol. 14, Issue 3 https://doi.org/10.1038/nbt0396-315	journal	March 1996
Synthesis of oligonucleotides on cellulose by a phosphotriester method Crea, Roberto; Horn, Thomas Nucleic Acids Research, Vol. 8, Issue 10 https://doi.org/10.1093/nar/8.10.2331	journal	January 1980
Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts Crossway, Anne; Oakes, Janette V.; Irvine, Jonathan M. MGG Molecular & General Genetics, Vol. 202, Issue 2 https://doi.org/10.1007/BF00331634	journal	February 1986
Peptides on phage: a vast library of peptides for identifying ligands Cwirla, S. E.; Peters, E. A.; Barrett, R. W. Proceedings of the National Academy of Sciences, Vol. 87, Issue 16, p. 6378-6382 https://doi.org/10.1073/pnas.87.16.6378	journal	August 1990
Genetically Engineered Fertile Indica-Rice Recovered from Protoplasts Datta, Swapan K.; Peterhans, Alex; Datta, Karabi Nature Biotechnology, Vol. 8, Issue 8 https://doi.org/10.1038/nbt0890-736	journal	August 1990
Acyl Carriers Used as Substrates by the Desaturases and Elongases Involved in Very Long-chain Polyunsaturated Fatty Acids Biosynthesis Reconstituted in Yeast Domergue, Frédéric; Abbadi, Amine; Ott, Claudia Journal of Biological Chemistry, Vol. 278, Issue 37 https://doi.org/10.1074/jbc.M305990200	journal	June 2003
Overlapping Destinations for Two Dual Targeted Glycyl-tRNA Synthetases in Arabidopsis thaliana and Phaseolus vulgaris Duchêne, Anne-Marie; Peeters, Nemo; Dietrich, André Journal of Biological Chemistry, Vol. 276, Issue 18 https://doi.org/10.1074/jbc.M011525200	journal	February 2001
Molecular Analysis of a Bifunctional Fatty Acid Conjugase/Desaturase from Tung. Implications for the Evolution of Plant Fatty Acid Diversity Dyer, John M.; Chapital, Dorselyn C.; Kuan, Jui-Chang W. Plant Physiology, Vol. 130, Issue 4 https://doi.org/10.1104/pp.102.010835	journal	November 2002
DNA polymerase fidelity and the polymerase chain reaction. Eckert, K. A.; Kunkel, T. A. Genome Research, Vol. 1, Issue 1 https://doi.org/10.1101/gr.1.1.17	journal	August 1991
Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence Emanuelsson, Olof; Nielsen, Henrik; Brunak, Søren Journal of Molecular Biology, Vol. 300, Issue 4 https://doi.org/10.1006/jmbi.2000.3903	journal	July 2000
The Suitability of Lipase from Rhizopus arrhizus delemar for Analysis of Fatty Acid Distribution in Dihexosyl Diglycerides, Phospholipids and Plant Sulfolipids Fischer, Werner; Heinz, Ernst; Zeus, Manfred Hoppe-Seyler´s Zeitschrift für physiologische Chemie, Vol. 354, Issue 2 https://doi.org/10.1515/bchm2.1973.354.2.1115	journal	January 1973
Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants Fromm, Michael E.; Morrish, Fionnuala; Armstrong, Charles Nature Biotechnology, Vol. 8, Issue 9 https://doi.org/10.1038/nbt0990-833	journal	September 1990
Characterization of A9 Acyl-lipid Desaturase Homologues from Arabidopsis thaliana Fukuchi-Mizutani, M.; Tasaka, Y.; Tanaka, Y. Plant and Cell Physiology, Vol. 39, Issue 2 https://doi.org/10.1093/oxfordjournals.pcp.a029364	journal	February 1998
Isolation of a ubiquitin-ribosomal protein gene (ubi3) from potato and expression of its promoter in transgenic plants Garbarino, Joan E.; Belknap, William R. Plant Molecular Biology, Vol. 24, Issue 1 https://doi.org/10.1007/BF00040579	journal	January 1994
Use of plant roots for phytoremediation and molecular farming Gleba, D.; Borisjuk, N. V.; Borisjuk, L. G. Proceedings of the National Academy of Sciences, Vol. 96, Issue 11 https://doi.org/10.1073/pnas.96.11.5973	journal	May 1999
SV40-transformed simian cells support the replication of early SV40 mutants Gluzman, Yakov Cell, Vol. 23, Issue 1, p. 175-182 https://doi.org/10.1016/0092-8674(81)90282-8	journal	January 1981
Effect of deletions in the cauliflower mosaic virus polyadenylation sequence on the choice of the polyadenylation sites in tobacco protoplasts Guerineau, Fran�ois; Brooks, Louise; Mullineaux, Philip MGG Molecular & General Genetics, Vol. 226-226, Issue 1-2 https://doi.org/10.1007/BF00273597	journal	April 1991
DGD1-independent biosynthesis of extraplastidic galactolipids after phosphate deprivation in Arabidopsis Hartel, H.; Dormann, P.; Benning, C. Proceedings of the National Academy of Sciences, Vol. 97, Issue 19, p. 10649-10654 https://doi.org/10.1073/pnas.180320497	journal	September 2000
Simple RNA enzymes with new and highly specific endoribonuclease activities Haseloff, Jim; Gerlach, Wayne L. Nature, Vol. 334, Issue 6183 https://doi.org/10.1038/334585a0	journal	August 1988
A Polyethylene Glycol-Mediated Protoplast Transformation System for Production of Fertile Transgenic Rice Plants Hayashimoto, Akio; Li, Zhijian; Murai, Norimoto Plant Physiology, Vol. 93, Issue 3 https://doi.org/10.1104/pp.93.3.857	journal	July 1990
Biolistic introduction of a synthetic Bt gene into elite maize Hill, M.; Launis, K.; Bowman, C. Euphytica, Vol. 85, Issue 1-3 https://doi.org/10.1007/BF00023939	journal	February 1995
Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA Transfer Hinchee, Maud A. W.; Connor-Ward, Dannette V.; Newell, Christine A. Nature Biotechnology, Vol. 6, Issue 8 https://doi.org/10.1038/nbt0888-915	journal	August 1988
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues Hochuli, E.; Döbeli, H.; Schacher, A. Journal of Chromatography A, Vol. 411 https://doi.org/10.1016/S0021-9673(00)93969-4	journal	January 1987
Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda Huse, W.; Sastry, L.; Iverson, S. Science, Vol. 246, Issue 4935 https://doi.org/10.1126/science.2531466	journal	December 1989
Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method ¹ Ike, Yoshimasa; Ikuta, Satoshi; Sato, Moriyuki Nucleic Acids Research, Vol. 11, Issue 2 https://doi.org/10.1093/nar/11.2.477	journal	January 1983
High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens Ishida, Yuji; Saito, Hideaki; Ohta, Shozo Nature Biotechnology, Vol. 14, Issue 6, p. 745-750 https://doi.org/10.1038/nbt0696-745	journal	June 1996
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin Itakura, K.; Hirose, T.; Crea, R. Science, Vol. 198, Issue 4321 https://doi.org/10.1126/science.412251	journal	December 1977
Synthesis and Use of Synthetic Oligonucleotides Itakura, Keiichi; Rossi, John J.; Wallace, R. Bruce Annual Review of Biochemistry, Vol. 53, Issue 1 https://doi.org/10.1146/annurev.bi.53.070184.001543	journal	June 1984
Regeneration of transgenic, microspore-derived, fertile barley Jähne, A.; Becker, D.; Brettschneider, R. Theoretical and Applied Genetics, Vol. 89, Issue 4 https://doi.org/10.1007/BF00225390	journal	October 1994
Novel seed lipid phenotypes in combinations of mutants altered in fatty acid biosynthesis inArabidopsis James, D. W.; Dooner, H. K. Theoretical and Applied Genetics, Vol. 82, Issue 4 https://doi.org/10.1007/BF00588591	journal	January 1991
Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Janknecht, R.; de Martynoff, G.; Lou, J. Proceedings of the National Academy of Sciences, Vol. 88, Issue 20 https://doi.org/10.1073/pnas.88.20.8972	journal	October 1991
Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis Joshi, C. P. Nucleic Acids Research, Vol. 15, Issue 23 https://doi.org/10.1093/nar/15.23.9627	journal	January 1987
An inspection of the domain between putative TATA box and translation start site in 79 plant genes Joshi, C. P. Nucleic Acids Research, Vol. 15, Issue 16 https://doi.org/10.1093/nar/15.16.6643	journal	January 1987
A short amino acid sequence able to specify nuclear location Kalderon, Daniel; Roberts, Bruce L.; Richardson, William D. Cell, Vol. 39, Issue 3, p. 499-509 https://doi.org/10.1016/0092-8674(84)90457-4	journal	December 1984
Light-Dependent Accumulation of Radiolabeled Plastid-Encoded Chlorophyll a -Apoproteins Requires Chlorophyll a : I. Analysis of Chlorophyll-Deficient Mutants and Phytochrome Involvement Klein, Robert R.; Gamble, Patricia E.; Mullet, John E. Plant Physiology, Vol. 88, Issue 4 https://doi.org/10.1104/pp.88.4.1246	journal	December 1988
Factors Influencing Gene Delivery into Zea Mays Cells by High–Velocity Microprojectiles Klein, T. M.; Gradziel, T.; Fromm, M. E. Nature Biotechnology, Vol. 6, Issue 5 https://doi.org/10.1038/nbt0588-559	journal	May 1988
Transformation of the developing barley endosperm by particle bombardment Knudsen, S�ren; M�ller, Martin Planta, Vol. 185, Issue 3 https://doi.org/10.1007/BF00201052	journal	October 1991
Continuous cultures of fused cells secreting antibody of predefined specificity Köhler, G.; Milstein, C. Nature, Vol. 256, Issue 5517, p. 495-497 https://doi.org/10.1038/256495a0	journal	August 1975
The production of monoclonal antibodies from human lymphocytes Kozbor, Danuta; Roder, John C. Immunology Today, Vol. 4, Issue 3, p. 72-79 https://doi.org/10.1016/0167-5699(83)90123-8	journal	March 1983
Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis Koziel, Michael G.; Beland, Gary L.; Bowman, Cindy Nature Biotechnology, Vol. 11, Issue 2 https://doi.org/10.1038/nbt0293-194	journal	February 1993
Transgenic Maize for the Control of European Corn Borer and Other Maize Insect Pests Koziel, Michael G.; Carozzi, Nadine B.; Desai, Nalini Annals of the New York Academy of Sciences, Vol. 792, Issue 1 Engineering P https://doi.org/10.1111/j.1749-6632.1996.tb32502.x	journal	May 1996
In vitro transformation of plant protoplasts with Ti-plasmid DNA Krens, F. A.; Molendijk, L.; Wullems, G. J. Nature, Vol. 296, Issue 5852 https://doi.org/10.1038/296072a0	journal	March 1982
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen Krogh, Anders; Larsson, Björn; von Heijne, Gunnar Journal of Molecular Biology, Vol. 305, Issue 3 https://doi.org/10.1006/jmbi.2000.4315	journal	January 2001
A Mutant of Arabidopsis Deficient in Desaturation of Palmitic Acid in Leaf Lipids Kunst, Ljerka; Browse, John; Somerville, Chris Plant Physiology, Vol. 90, Issue 3 https://doi.org/10.1104/pp.90.3.943	journal	July 1989
Targeting of T7 RNA polymerase to tobacco nuclei mediated by an SV40 nuclear location signal Lassner, Michael W.; Jones, Aubrey; Daubert, Steve Plant Molecular Biology, Vol. 17, Issue 2 https://doi.org/10.1007/BF00039497	journal	August 1991
Intron enhancement of gene expression and the splicing efficiency of introns in maize cells Luehrsen, KennethR.; Walbot, Virginia MGG Molecular & General Genetics, Vol. 225, Issue 1 https://doi.org/10.1007/BF00282645	journal	January 1991
A desaturase-like protein from white spruce is a Δ⁹ desaturase Marillia, Elizabeth-France; Giblin, E. Michael; Covello, Patrick S. FEBS Letters, Vol. 526, Issue 1-3, p. 49-52 https://doi.org/10.1016/S0014-5793(02)03112-5	journal	August 2002
The synthesis of oligodeoxyprimidines on a polymer support Matteucci, M. D.; Caruthers, M. H. Tetrahedron Letters, Vol. 21, Issue 8, p. 719-722 https://doi.org/10.1016/S0040-4039(00)71455-9	journal	January 1980
Stable Transformation of Soybean (Glycine Max) by Particle Acceleration McCabe, Dennis E.; Swain, William F.; Martinell, Brian J. Nature Biotechnology, Vol. 6, Issue 8 https://doi.org/10.1038/nbt0888-923	journal	August 1988
Toward a Functional Catalog of the Plant Genome. A Survey of Genes for Lipid Biosynthesis Mekhedov, Sergei; de Ilárduya, Oskar Martı́nez; Ohlrogge, John Plant Physiology, Vol. 122, Issue 2 https://doi.org/10.1104/pp.122.2.389	journal	February 2000
Ribozymes Targeted to Stearoyl–ACP Δ9 Desaturase mRNA Produce Heritable Increases of Stearic Acid in Transgenic Maize Leaves Merlo, Ann Owens; Cowen, Neil; Delate, Tom The Plant Cell, Vol. 10, Issue 10 https://doi.org/10.1105/tpc.10.10.1603	journal	October 1998
Tales of poly(A): a review Munroe, David; Jacobson, Allan Gene, Vol. 91, Issue 2, p. 151-158 https://doi.org/10.1016/0378-1119(90)90082-3	journal	July 1990
Codon usage in plant genes Murray, Elizabeth E.; Lotzer, Jeff; Eberle, Mary Nucleic Acids Research, Vol. 17, Issue 2 https://doi.org/10.1093/nar/17.2.477	journal	January 1989
Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. Napoli, C.; Lemieux, C.; Jorgensen, R. The Plant Cell https://doi.org/10.1105/tpc.2.4.279	journal	April 1990
DNA synthesis Narang, Saran A. Tetrahedron, Vol. 39, Issue 1, p. 3-22 https://doi.org/10.1016/S0040-4020(01)97623-9	journal	January 1983
Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs+ Nehra, Narender S.; Chibbar, Ravindra N.; Leung, Nick The Plant Journal, Vol. 5, Issue 2 https://doi.org/10.1046/j.1365-313X.1994.05020285.x	journal	February 1994
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter Odell, Joan T.; Nagy, Ferenc; Chua, Nam-Hai Nature, Vol. 313, Issue 6005, p. 810-812 https://doi.org/10.1038/313810a0	journal	February 1985
A strategy for a convergent synthesis of N-linked glycopeptides on a solid support Roberge, J.; Beebe, X.; Danishefsky, S. Science, Vol. 269, Issue 5221 https://doi.org/10.1126/science.7618080	journal	July 1995
Vectors for selective expression of cloned DNAs by T7 RNA polymerase Rosenberg, Alan H.; Lade, Barbara N.; Dao-shan, Chui Gene, Vol. 56, Issue 1 https://doi.org/10.1016/0378-1119(87)90165-X	journal	January 1987
Linoleate and α-linolenate synthesis by isolated spinach (Spinacia oleracea) chloroplasts Roughan, P. G.; Mudd, J. B.; McManus, T. T. Biochemical Journal, Vol. 184, Issue 3 https://doi.org/10.1042/bj1840571	journal	December 1979
Metabolism of exogenous long-chain fatty acids by spinach leaves Roughan, P. Grattan; Thompson, Guy A.; Cho, Sung Ho Archives of Biochemistry and Biophysics, Vol. 259, Issue 2, p. 481-496 https://doi.org/10.1016/0003-9861(87)90515-7	journal	December 1987
A dissection of the cauliflower mosaic virus polyadenylation signal. Sanfacon, H.; Brodmann, P.; Hohn, T. Genes & Development, Vol. 5, Issue 1 https://doi.org/10.1101/gad.5.1.141	journal	January 1991
Delivery of Substances into Cells and Tissues Using a Particle Bombardment Process Sanford, John C.; Klein, Theodore M.; Wolf, Edward D. Particulate Science and Technology, Vol. 5, Issue 1 https://doi.org/10.1080/02726358708904533	journal	January 1987
Fertile transgenic rice plants regenerated from transformed protoplasts Shimamoto, Ko; Terada, Rie; Izawa, Takeshi Nature, Vol. 338, Issue 6212, p. 274-276 https://doi.org/10.1038/338274a0	journal	March 1989
Expression of a truncated tomato polygalacturonase gene inhibits expression of the endogenous gene in transgenic plants Smith, C. J. S.; Watson, C. F.; Bird, C. R. MGG Molecular & General Genetics, Vol. 224, Issue 3 https://doi.org/10.1007/BF00262443	journal	December 1990
Bialaphos selection of stable transformants from maize cell culture Spencer, T. M.; Gordon-Kamm, W. J.; Daines, R. J. Theoretical and Applied Genetics, Vol. 79, Issue 5 https://doi.org/10.1007/BF00226875	journal	May 1990
Desaturases fused to their electron donor Sperling, Petra; Heinz, Ernst European Journal of Lipid Science and Technology, Vol. 103, Issue 3, p. 158-180 https://doi.org/10.1002/1438-9312(200103)103:3<158::AID-EJLT158>3.0.CO;2-1	journal	March 2001
Transgenic Plants as Tools to Study the Molecular Organization of Plant Genes St. Schell, J. Science, Vol. 237, Issue 4819 https://doi.org/10.1126/science.237.4819.1176	journal	September 1987
Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation. Staub, J. M.; Maliga, P. The Plant Cell, Vol. 4, Issue 1 https://doi.org/10.1105/tpc.4.1.39	journal	January 1992
Rapid evolution of a protein in vitro by DNA shuffling Stemmer, Willem P. C. Nature, Vol. 370, Issue 6488 https://doi.org/10.1038/370389a0	journal	August 1994
hpRNA-Mediated Targeting of the Arabidopsis FAD2 Gene Gives Highly Efficient and Stable Silencing Stoutjesdijk, Peter A.; Singh, Surinder P.; Liu, Qing Plant Physiology, Vol. 129, Issue 4 https://doi.org/10.1104/pp.006353	journal	July 2002
Isoforms of acyl carrier protein involved in seed‐specific fatty acid synthesis Suh, Mi Chung; Schultz, David J.; Ohlrogge, John B. The Plant Journal, Vol. 17, Issue 6 https://doi.org/10.1046/j.1365-313X.1999.00418.x	journal	March 1999
Stable transformation of plastids in higher plants. Svab, Z.; Hajdukiewicz, P.; Maliga, P. Proceedings of the National Academy of Sciences, Vol. 87, Issue 21 https://doi.org/10.1073/pnas.87.21.8526	journal	November 1990
High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Svab, Z.; Maliga, P. Proceedings of the National Academy of Sciences, Vol. 90, Issue 3 https://doi.org/10.1073/pnas.90.3.913	journal	February 1993
Why are proteins marginally stable? Taverna, Darin M.; Goldstein, Richard A. Proteins: Structure, Function, and Genetics, Vol. 46, Issue 1 https://doi.org/10.1002/prot.10016	journal	December 2001
Isolation and Characterization of a Gene Affecting Fatty Acid Elongation in Saccharomyces cerevisiae Toke, David A.; Martin, Charles E. Journal of Biological Chemistry, Vol. 271, Issue 31 https://doi.org/10.1074/jbc.271.31.18413	journal	August 1996
The HMMTOP transmembrane topology prediction server Tusnady, G. E.; Simon, I. Bioinformatics, Vol. 17, Issue 9 https://doi.org/10.1093/bioinformatics/17.9.849	journal	September 2001
Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants Umbeck, Paul; Johnson, Gail; Barton, Ken Bio/Technology, Vol. 5, Issue 3 https://doi.org/10.1038/nbt0387-263	journal	March 1987
Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. van der Krol, A. R.; Mur, L. A.; Beld, M. The Plant Cell, Vol. 2, Issue 4 https://doi.org/10.1105/tpc.2.4.291	journal	April 1990
Rapid Production of Transgenic Wheat Plants by Direct Bombardment of Cultured Immature Embryos Vasil, Vimla; Srivastava, Vibha; Castillo, Ana M. Nature Biotechnology, Vol. 11, Issue 12 https://doi.org/10.1038/nbt1293-1553	journal	December 1993
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers Vieira, Jeffrey; Messing, Joachim Gene, Vol. 19, Issue 3, p. 259-268 https://doi.org/10.1016/0378-1119(82)90015-4	journal	October 1982
Photoinhibition in Mutants of Arabidopsis Deficient in Thylakoid Unsaturation Vijayan, Perumal; Browse, John Plant Physiology, Vol. 129, Issue 2 https://doi.org/10.1104/pp.004341	journal	May 2002
The role of enhancers in the regulation of cell-type-specific transcriptional control Voss, Stephan D.; Schlokat, Uwe; Gruss, Peter Trends in Biochemical Sciences, Vol. 11, Issue 7, p. 287-289 https://doi.org/10.1016/0968-0004(86)90031-9	journal	July 1986
Generation of glycerophospholipid molecular species in the yeastSaccharomyces cerevisiae. Fatty acid pattern of phospholipid classes and selective acyl turnover atsn-1 andsn-2 positions Wagner, S.; Paltauf, F. Yeast, Vol. 10, Issue 11 https://doi.org/10.1002/yea.320101106	journal	November 1994
Generation of Large Numbers of Independently Transformed Fertile Barley Plants Wan, Y.; Lemaux, P. G. Plant Physiology, Vol. 104, Issue 1 https://doi.org/10.1104/pp.104.1.37	journal	January 1994
Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum) Weeks, J. T.; Anderson, O. D.; Blechl, A. E. Plant Physiology, Vol. 102, Issue 4 https://doi.org/10.1104/pp.102.4.1077	journal	August 1993
Foreign Genes in Plants: Transfer, Structure, Expression, and Applications Weising, K.; Schell, J.; Kahl, G. Annual Review of Genetics, Vol. 22, Issue 1 https://doi.org/10.1146/annurev.ge.22.120188.002225	journal	December 1988
Construct design for efficient, effective and high-throughput gene silencing in plants: Construct design for gene silencing Wesley, S. Varsha; Helliwell, Christopher A.; Smith, Neil A. The Plant Journal, Vol. 27, Issue 6 https://doi.org/10.1046/j.1365-313X.2001.01105.x	journal	September 2001
A cassette containing the bar gene of Streptomyces hygroscopicus : a selectable marker for plant transformation White, Janet; Chang, Sheng-Yung P.; Bibb, Maureen J. Nucleic Acids Research, Vol. 18, Issue 4 https://doi.org/10.1093/nar/18.4.1062	journal	January 1990
The structure of an antigenic determinant in a protein Wilson, Ian A.; Niman, Henry L.; Houghten, Richard A. Cell, Vol. 37, Issue 3, p. 767-778 https://doi.org/10.1016/0092-8674(84)90412-4	journal	July 1984
The ligation amplification reaction (LAR)—Amplification of specific DNA sequences using sequential rounds of template-dependent ligation Wu, Dan Y.; Wallace, R. Bruce Genomics, Vol. 4, Issue 4, p. 560-569 https://doi.org/10.1016/0888-7543(89)90280-2	journal	May 1989
Determination of double-bond positions in methylene-interrupted dienoic fatty acids by GC-MS as their dimethyl disulfide adducts Yamamoto, Kohei; Shibahara, Akira; Nakayama, Takao Chemistry and Physics of Lipids, Vol. 60, Issue 1, p. 39-50 https://doi.org/10.1016/0009-3084(91)90013-2	journal	November 1991
Expression of the Arabidopsis ADS1 gene in Brassica juncea results in a decreased level of total saturated fatty acids: ��9 desaturase over-expression in B. juncea Yao, Kening; Bacchetto, Roberto G.; Lockhart, Katherine M. Plant Biotechnology Journal, Vol. 1, Issue 3 https://doi.org/10.1046/j.1467-7652.2003.00021.x	journal	April 2003
Optimization of DNA shuffling for high fidelity recombination Zhao, H. Nucleic Acids Research, Vol. 25, Issue 6 https://doi.org/10.1093/nar/25.6.1307	journal	March 1997

Similar Records

ADS genes for reducing saturated fatty acid levels in seed oils

Patent · Tue Feb 02 00:00:00 EST 2010 · OSTI ID:1127126

Heilmann, Ingo H; Shanklin, John

Metabolic engineering of seeds can achieve levels of omega-7 fatty acids comparable to the highest levels found in natural plant sources

Journal Article · Wed Dec 01 00:00:00 EST 2010 · Plant Physiology · OSTI ID:1127126

Nguyen, H T; Shanklin, J; Mishra, G; +4 more

Characterization and analysis of the cotton cyclopropane fatty acid synthase family and their contribution to cyclopropane fatty acid synthesis

Journal Article · Sun May 01 00:00:00 EDT 2011 · BMC Plant Biology · OSTI ID:1127126

H, Yu X; Shanklin, J; Rawat, R

Related Subjects

59 BASIC BIOLOGICAL SCIENCES

Title: ADS genes for reducing saturated fatty acid levels in seed oils

Citation Formats

References (154)

Similar Records

Related Subjects