Luminescent Lanthanide Reporters for High-Sensitivity Novel Bioassays

Anstey, Mitchell R.; Fruetel, Julia A.; Foster, Michael E.; Hayden, Carl C.; Buckley, Heather L.; Arnold, John

doi:10.2172/1127114

Title: Luminescent Lanthanide Reporters for High-Sensitivity Novel Bioassays

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Abstract

Biological imaging and assay technologies rely on fluorescent organic dyes as reporters for a number of interesting targets and processes. However, limitations of organic dyes such as small Stokes shifts, spectral overlap of emission signals with native biological fluorescence background, and photobleaching have all inhibited the development of highly sensitive assays. To overcome the limitations of organic dyes for bioassays, we propose to develop lanthanide-based luminescent dyes and demonstrate them for molecular reporting applications. This relatively new family of dyes was selected for their attractive spectral and chemical properties. Luminescence is imparted by the lanthanide atom and allows for relatively simple chemical structures that can be tailored to the application. The photophysical properties offer unique features such as narrow and non-overlapping emission bands, long luminescent lifetimes, and long wavelength emission, which enable significant sensitivity improvements over organic dyes through spectral and temporal gating of the luminescent signal.Growth in this field has been hindered due to the necessary advanced synthetic chemistry techniques and access to experts in biological assay development. Our strategy for the development of a new lanthanide-based fluorescent reporter system is based on chelation of the lanthanide metal center using absorbing chromophores. Our first strategy involves "Click" chemistry tomore »« less

Authors:

Anstey, Mitchell R. ^[1]; Fruetel, Julia A. ^[1]; Foster, Michael E. ^[1]; Hayden, Carl C. ^[1]; Buckley, Heather L. ^[1]; Arnold, John ^[1]

Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Publication Date:: Sun Sep 01 00:00:00 EDT 2013

Research Org.:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Org.:: USDOE National Nuclear Security Administration (NNSA)

OSTI Identifier:: 1127114

Report Number(s):: SAND2013-7827
506670

DOE Contract Number:: AC04-94AL85000

Resource Type:: Technical Report

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Anstey, Mitchell R., Fruetel, Julia A., Foster, Michael E., Hayden, Carl C., Buckley, Heather L., and Arnold, John. Luminescent Lanthanide Reporters for High-Sensitivity Novel Bioassays.  United States: N. p., 2013. 
        Web.  doi:10.2172/1127114.

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                    Anstey, Mitchell R., Fruetel, Julia A., Foster, Michael E., Hayden, Carl C., Buckley, Heather L., & Arnold, John. Luminescent Lanthanide Reporters for High-Sensitivity Novel Bioassays.  United States.  https://doi.org/10.2172/1127114

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                    Anstey, Mitchell R., Fruetel, Julia A., Foster, Michael E., Hayden, Carl C., Buckley, Heather L., and Arnold, John. 2013.  
        "Luminescent Lanthanide Reporters for High-Sensitivity Novel Bioassays".  United States.  https://doi.org/10.2172/1127114.  https://www.osti.gov/servlets/purl/1127114.

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@article{osti_1127114,

  title        = {Luminescent Lanthanide Reporters for High-Sensitivity Novel Bioassays},

  author       = {Anstey, Mitchell R. and Fruetel, Julia A. and Foster, Michael E. and Hayden, Carl C. and Buckley, Heather L. and Arnold, John},

  abstractNote = {Biological imaging and assay technologies rely on fluorescent organic dyes as reporters for a number of interesting targets and processes. However, limitations of organic dyes such as small Stokes shifts, spectral overlap of emission signals with native biological fluorescence background, and photobleaching have all inhibited the development of highly sensitive assays. To overcome the limitations of organic dyes for bioassays, we propose to develop lanthanide-based luminescent dyes and demonstrate them for molecular reporting applications. This relatively new family of dyes was selected for their attractive spectral and chemical properties. Luminescence is imparted by the lanthanide atom and allows for relatively simple chemical structures that can be tailored to the application. The photophysical properties offer unique features such as narrow and non-overlapping emission bands, long luminescent lifetimes, and long wavelength emission, which enable significant sensitivity improvements over organic dyes through spectral and temporal gating of the luminescent signal.Growth in this field has been hindered due to the necessary advanced synthetic chemistry techniques and access to experts in biological assay development. Our strategy for the development of a new lanthanide-based fluorescent reporter system is based on chelation of the lanthanide metal center using absorbing chromophores. Our first strategy involves "Click" chemistry to develop 3-fold symmetric chelators and the other involves use of a new class of tetrapyrrole ligands called corroles. This two-pronged approach is geared towards the optimization of chromophores to enhance light output.},

  doi          = {10.2172/1127114},

  url          = {https://www.osti.gov/biblio/1127114},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Sep 01 00:00:00 EDT 2013},

  month        = {Sun Sep 01 00:00:00 EDT 2013}

}

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