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Title: Oxygen-resistant hydrogenases and methods for designing and making same

Abstract

The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.

Inventors:
; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1127083
Patent Number(s):
8,663,958
Application Number:
12/360,756
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO) NREL
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

King, Paul, Ghirardi, Maria Lucia, and Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same. United States: N. p., 2014. Web.
King, Paul, Ghirardi, Maria Lucia, & Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same. United States.
King, Paul, Ghirardi, Maria Lucia, and Seibert, Michael. Tue . "Oxygen-resistant hydrogenases and methods for designing and making same". United States. https://www.osti.gov/servlets/purl/1127083.
@article{osti_1127083,
title = {Oxygen-resistant hydrogenases and methods for designing and making same},
author = {King, Paul and Ghirardi, Maria Lucia and Seibert, Michael},
abstractNote = {The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {3}
}

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Works referenced in this record:

Prediction of protein function from protein sequence and structure
journal, January 1999

  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

Classification and phylogeny of hydrogenases
journal, August 2001