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Title: Recombinant host cells and media for ethanol production

Abstract

Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.

Inventors:
; ; ;
Publication Date:
Research Org.:
University of Florida Research Foundation, Inc., Gainesville, FL, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1124787
Patent Number(s):
8,652,817
Application Number:
11/922,631
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
DOE Contract Number:  
FG02-96ER20222
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Wood, Brent E, Ingram, Lonnie O, Yomano, Lorraine P, and York, Sean W. Recombinant host cells and media for ethanol production. United States: N. p., 2014. Web.
Wood, Brent E, Ingram, Lonnie O, Yomano, Lorraine P, & York, Sean W. Recombinant host cells and media for ethanol production. United States.
Wood, Brent E, Ingram, Lonnie O, Yomano, Lorraine P, and York, Sean W. 2014. "Recombinant host cells and media for ethanol production". United States. https://www.osti.gov/servlets/purl/1124787.
@article{osti_1124787,
title = {Recombinant host cells and media for ethanol production},
author = {Wood, Brent E and Ingram, Lonnie O and Yomano, Lorraine P and York, Sean W},
abstractNote = {Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.},
doi = {},
url = {https://www.osti.gov/biblio/1124787}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 18 00:00:00 EST 2014},
month = {Tue Feb 18 00:00:00 EST 2014}
}

Works referenced in this record:

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Biobased Industrial Products
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Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration
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Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli
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Potential Synergies and Challenges in Refining Cellulosic Biomass to Fuels, Chemicals, and Power
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Ultrasound Stimulates Ethanol Production during the Simultaneous Saccharification and Fermentation of Mixed Waste Office Paper
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Improved strains of recombinant Escherichia coli for ethanol production from sugar mixtures
journal, April 1995


Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production
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Cloning, Characterization, and Functional Expression of the Klebsiella oxytoca Xylodextrin Utilization Operon (xynTB) in Escherichia coli
journal, October 2003


Chemically defined media for commercial fermentations
journal, April 1999