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Title: The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis

Abstract

Great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, though the role of interactions among these variables is largely nebulous. We also describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopic and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. Furthermore, this phenomenon introduces themore » potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.« less

Authors:
 [1];  [1];  [2];  [1]
  1. Sandia National Lab. (SNL-CA), Livermore, CA (United States)
  2. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1122967
Report Number(s):
SAND2013-10548J
Journal ID: ISSN 0921-8971; PII: 331
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Applied Phycology
Additional Journal Information:
Journal Volume: 27; Journal Issue: 1; Journal ID: ISSN 0921-8971
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microalgae; dunaliella viridis; algae bioproducts; semi-continuous culture; photo-osmotic adaptation; lipid micoparticles; algal milking

Citation Formats

Davis, Ryan W., Carvalho, Benjamin J., Jones, Howland D. T., and Singh, Seema. The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis. United States: N. p., 2014. Web. doi:10.1007/s10811-014-0331-5.
Davis, Ryan W., Carvalho, Benjamin J., Jones, Howland D. T., & Singh, Seema. The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis. United States. doi:10.1007/s10811-014-0331-5.
Davis, Ryan W., Carvalho, Benjamin J., Jones, Howland D. T., and Singh, Seema. Tue . "The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis". United States. doi:10.1007/s10811-014-0331-5. https://www.osti.gov/servlets/purl/1122967.
@article{osti_1122967,
title = {The role of photo-osmotic adaptation in semi-continuous culture and lipid particle release from Dunaliella viridis},
author = {Davis, Ryan W. and Carvalho, Benjamin J. and Jones, Howland D. T. and Singh, Seema},
abstractNote = {Great efforts have been made to elucidate the phenotypic responses of alga to varying levels of nutrients, osmotic environments, and photosynthetically active radiation intensities, though the role of interactions among these variables is largely nebulous. We also describe a general method for establishing and maintaining semi-continuous cultures of the halophilic microalgal production strain, Dunaliella viridis, that is independent of variations in salinity and illumination intensity. Using this method, the cultures were evaluated to elucidate the overlapping roles of photosynthetic and osmotic adaptation on the accumulation and compositional variation of the biomass, photosynthetic productivity, and physiological biomarkers, as well as spectroscopic and morphological details at the single-cell level. Correlation matrices defining the relationships among the observables and based on variation of the illumination intensity and salinity were constructed for predicting bioproduct yields for varying culture conditions. Following maintenance of stable cultures for 6-week intervals, phenotypic responses to photo-osmotic drift were explored using a combination of single-cell hyperspectral fluorescence imaging and flow cytometry. In addition to morphological changes, release of lipid microparticles from the cells that is disproportionate to cell lysis was observed under hypotonic drift, indicating the existence of a reversible membrane permeation mechanism in Dunaliella. Furthermore, this phenomenon introduces the potential for low-cost strategies for recovering lipids and pigments from the microalgae by minimizing the requirement for energy intensive harvesting and dewatering of the biomass. The results should be applicable to outdoor culture, where seasonal changes resulting in variable solar flux and precipitation and evaporation rates are anticipated.},
doi = {10.1007/s10811-014-0331-5},
journal = {Journal of Applied Phycology},
issn = {0921-8971},
number = 1,
volume = 27,
place = {United States},
year = {2014},
month = {5}
}

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