Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface supports for high avidity microbial capture

Hansen, Ryan R; Hinestrosa Salazar, Juan P; Shubert, Katherine R; Morrell, Jennifer L.; Pelletier, Dale A; Messman, Jamie M; Lokitz, Bradley S; Retterer, Scott T

doi:10.1021/bm4011358

Title: Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface supports for high avidity microbial capture

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Abstract

Microbial exopolysaccharides (EPS) play a critical and dynamic role in shaping the interactions between microbial community members and their local environment. The capture of targeted microbes using surface immobilized lectins that recognize specific extracellular oligosaccharide moieties offers a non-destructive method for functional characterization based on EPS content. In this report, we evaluate the use of the block co-polymer, poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA), as a surface support for lectin-specific microbial capture. Arrays of circular polymer supports ten micron in diameter were generated on silicon substrates to provide discrete, covalent coupling sites for Triticum vulgare and Lens culinaris lectins. These supports promoted microbe adhesion and colony formation in a lectin-specific manner. Silicon posts with similar topography containing only physisorbed lectins showed significantly less activity. These results demonstrate that micropatterned PGMA-b-PVDMA supports provide a unique platform for microbial capture and screening based on EPS content by combining high avidity lectin surfaces with three-dimensional topography.

Authors:

Hansen, Ryan R ^[1]; Hinestrosa Salazar, Juan P ^[1]; Shubert, Katherine R ^[1]; Morrell, Jennifer L. ^[2]; Pelletier, Dale A ^[1]; Messman, Jamie M ^[1]; Lokitz, Bradley S ^[1]; Retterer, Scott T ^[1]

ORNL
University of Tennessee, Knoxville (UTK)

Publication Date:: 2013-01-01

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1115366

DOE Contract Number:: DE-AC05-00OR22725

Resource Type:: Journal Article

Journal Name:: Biomacromolecules

Additional Journal Information:: Journal Volume: 14; Journal Issue: 10; Journal ID: ISSN 1525--7797

Country of Publication:: United States

Language:: English

Subject:: Lectins; block co-polymers; exopolysaccharide; plant growth promoting rhizobacteria; micropatterning; cellular adhesion

Citation Formats


                    Hansen, Ryan R, Hinestrosa Salazar, Juan P, Shubert, Katherine R, Morrell, Jennifer L., Pelletier, Dale A, Messman, Jamie M, Kilbey, II, S Michael, Lokitz, Bradley S, and Retterer, Scott T. Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface supports for high avidity microbial capture.  United States: N. p., 2013. 
        Web.  doi:10.1021/bm4011358.

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                    Hansen, Ryan R, Hinestrosa Salazar, Juan P, Shubert, Katherine R, Morrell, Jennifer L., Pelletier, Dale A, Messman, Jamie M, Kilbey, II, S Michael, Lokitz, Bradley S, & Retterer, Scott T. Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface supports for high avidity microbial capture.  United States.  https://doi.org/10.1021/bm4011358

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                    Hansen, Ryan R, Hinestrosa Salazar, Juan P, Shubert, Katherine R, Morrell, Jennifer L., Pelletier, Dale A, Messman, Jamie M, Kilbey, II, S Michael, Lokitz, Bradley S, and Retterer, Scott T. 2013.  
        "Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface supports for high avidity microbial capture".  United States.  https://doi.org/10.1021/bm4011358.

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@article{osti_1115366,

  title        = {Lectin-functionalized poly(glycidyl methacrylate)-block-poly(vinyldimethyl azlactone) surface supports for high avidity microbial capture},

  author       = {Hansen, Ryan R and Hinestrosa Salazar, Juan P and Shubert, Katherine R and Morrell, Jennifer L. and Pelletier, Dale A and Messman, Jamie M and Kilbey, II, S Michael and Lokitz, Bradley S and Retterer, Scott T},

  abstractNote = {Microbial exopolysaccharides (EPS) play a critical and dynamic role in shaping the interactions between microbial community members and their local environment. The capture of targeted microbes using surface immobilized lectins that recognize specific extracellular oligosaccharide moieties offers a non-destructive method for functional characterization based on EPS content. In this report, we evaluate the use of the block co-polymer, poly(glycidyl methacrylate)-block-4,4-dimethyl-2-vinylazlactone (PGMA-b-PVDMA), as a surface support for lectin-specific microbial capture. Arrays of circular polymer supports ten micron in diameter were generated on silicon substrates to provide discrete, covalent coupling sites for Triticum vulgare and Lens culinaris lectins. These supports promoted microbe adhesion and colony formation in a lectin-specific manner. Silicon posts with similar topography containing only physisorbed lectins showed significantly less activity. These results demonstrate that micropatterned PGMA-b-PVDMA supports provide a unique platform for microbial capture and screening based on EPS content by combining high avidity lectin surfaces with three-dimensional topography.},

  doi          = {10.1021/bm4011358},

  url          = {https://www.osti.gov/biblio/1115366},
  journal      = {Biomacromolecules},

  issn         = {1525--7797},

  number       = 10,

  volume       = 14,

  place        = {United States},

  year         = {2013},

  month        = {1}

}

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