Solubilization of minerals by bacteria: Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur

Blake, II, R C; Shute, E A; Howard, G T

Title: Solubilization of minerals by bacteria: Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur

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Abstract

Thiobacillus ferrooxidans is an obligate acidophile that respires aerobically on pyrite, elemental sulfur, or soluble ferrous ions. The electrophoretic mobility of the bacterium was determined by laser Doppler velocimetry under physiological conditions. When grown on pyrite or ferrous ions, washed cells were negatively charged at pH 2.0. The density of the negative charge depended on whether the conjugate base was sulfate, perchlorate, chloride, or nitrate. The addition of ferric ions shifted the net charge on the surface asymptotically to a positive value. When grown on elemental sulfur, washed cells were close to their isoelectric point at pH 2.0. Both pyrite and colloidal sulfur were negatively charged under the same conditions. The electrical double layer around the bacterial cells under physiological conditions exerted minimal electrostatic repulsion in possible interactions between the cell and either of its charged insoluble substrates. When Thiobacillus ferrooxidans was mixed with either pyrite or colloidal sulfur at pH 2.0, the mobility spectra of the free components disappeared with time to be replaced with a new colloidal particle whose electrophoretic properties were intermediate between those of the starting components. This new particle had the charge and size properties anticipated for a complex between the bacterium and its insolublemore »« less

Authors:

Blake, II, R C; Shute, E A; Howard, G T ^[1]

Meharry Medical College, Nashville, TN (United States)

Publication Date:: Thu Sep 01 00:00:00 EDT 1994

OSTI Identifier:: 111392

DOE Contract Number:: FG05-85ER13339; FG05-92ER20087

Resource Type:: Journal Article

Journal Name:: Applied and Environmental Microbiology

Additional Journal Information:: Journal Volume: 60; Journal Issue: 9; Other Information: PBD: Sep 1994

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; 54 ENVIRONMENTAL SCIENCES; BACTERIA; BIOLOGICAL PATHWAYS; MINERALS; SOLUBILITY; CULTURE MEDIA; PH VALUE; CHEMICAL COMPOSITION; IRON; SULFUR; PYRITE

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                    Blake, II, R C, Shute, E A, and Howard, G T. Solubilization of minerals by bacteria: Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur.  United States: N. p., 1994. 
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                    Blake, II, R C, Shute, E A, & Howard, G T. Solubilization of minerals by bacteria: Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur.  United States.

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                    Blake, II, R C, Shute, E A, and Howard, G T. 1994.  
        "Solubilization of minerals by bacteria: Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur".  United States.

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@article{osti_111392,

  title        = {Solubilization of minerals by bacteria: Electrophoretic mobility of Thiobacillus ferrooxidans in the presence of iron, pyrite, and sulfur},

  author       = {Blake, II, R C and Shute, E A and Howard, G T},

  abstractNote = {Thiobacillus ferrooxidans is an obligate acidophile that respires aerobically on pyrite, elemental sulfur, or soluble ferrous ions. The electrophoretic mobility of the bacterium was determined by laser Doppler velocimetry under physiological conditions. When grown on pyrite or ferrous ions, washed cells were negatively charged at pH 2.0. The density of the negative charge depended on whether the conjugate base was sulfate, perchlorate, chloride, or nitrate. The addition of ferric ions shifted the net charge on the surface asymptotically to a positive value. When grown on elemental sulfur, washed cells were close to their isoelectric point at pH 2.0. Both pyrite and colloidal sulfur were negatively charged under the same conditions. The electrical double layer around the bacterial cells under physiological conditions exerted minimal electrostatic repulsion in possible interactions between the cell and either of its charged insoluble substrates. When Thiobacillus ferrooxidans was mixed with either pyrite or colloidal sulfur at pH 2.0, the mobility spectra of the free components disappeared with time to be replaced with a new colloidal particle whose electrophoretic properties were intermediate between those of the starting components. This new particle had the charge and size properties anticipated for a complex between the bacterium and its insoluble substrates. The utility of such measurements for the study of the interactions of chemolithotrophic bacteria with their insoluble substrates is discussed. 34 refs., 9 figs., 1 tab.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/111392},
  journal      = {Applied and Environmental Microbiology},
number       = 9,

  volume       = 60,

  place        = {United States},

  year         = {Thu Sep 01 00:00:00 EDT 1994},

  month        = {Thu Sep 01 00:00:00 EDT 1994}

}

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