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Title: Fatty acid-producing hosts

Abstract

Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.

Inventors:
;
Publication Date:
Research Org.:
Wisconsin Alumni Research Foundation, Madison, WI, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1113235
Patent Number(s):
8,617,856
Application Number:
12/984,343
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
DOE Contract Number:  
FC02-07ER64494; FG02-03ER15468
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Pfleger, Brian F, and Lennen, Rebecca M. Fatty acid-producing hosts. United States: N. p., 2013. Web.
Pfleger, Brian F, & Lennen, Rebecca M. Fatty acid-producing hosts. United States.
Pfleger, Brian F, and Lennen, Rebecca M. Tue . "Fatty acid-producing hosts". United States. doi:. https://www.osti.gov/servlets/purl/1113235.
@article{osti_1113235,
title = {Fatty acid-producing hosts},
author = {Pfleger, Brian F and Lennen, Rebecca M},
abstractNote = {Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 31 00:00:00 EST 2013},
month = {Tue Dec 31 00:00:00 EST 2013}
}

Patent:

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