Fatty acid-producing hosts

Pfleger, Brian F; Lennen, Rebecca M

Fatty acid-producing hosts

Patent · 31 December 2013

OSTI ID:1113235

Pfleger, Brian F; Lennen, Rebecca M

Described are hosts for overproducing a fatty acid product such as a fatty acid. The hosts include an exogenous nucleic acid encoding a thioesterase and, optionally, an exogenous nucleic acid encoding an acetyl-CoA carboxylase, wherein an acyl-CoA synthetase in the hosts are functionally delected. The hosts prefereably include the nucleic acid encoding the thioesterase at an intermediate copy number. The hosts are preferably recominantly stable and growth-competent at 37.degree. C. Methods of producing a fatty acid product comprising culturing such hosts at 37.degree. C. are also described.

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Research Organization:: Wisconsin Alumni Research Foundation, Madison, WI, USA

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494; FG02-03ER15468

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Number(s):: 8,617,856

Application Number:: 12/984,343

OSTI ID:: 1113235

Country of Publication:: United States

Language:: English

References (77)

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