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Office of Scientific and Technical Information
 

Transcription factor-based biosensor

Patent ·
OSTI ID:1097260
;
The present invention provides for a system comprising a BmoR transcription factor, a .sigma..sup.54-RNA polymerase, and a pBMO promoter operatively linked to a reporter gene, wherein the pBMO promoter is capable of expression of the reporter gene with an activated form of the BmoR and the .sigma..sup.54-RNA polymerase.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
8,552,169
Application Number:
12/767,649
OSTI ID:
1097260
Country of Publication:
United States
Language:
English

References (8)

[10] Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast book January 1983
In a class of its own ? the RNA polymerase sigma factor ?; 54 (? N ) journal December 1993
Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. journal July 1995
The Bacterial Enhancer-Dependent sigma 54 (sigma N) Transcription Factor journal August 2000
Product and product-independent induction of butane oxidation in Pseudomonas butanovora journal September 2005
Involvement of BmoR and BmoG in n-alkane metabolism in ‘Pseudomonas butanovora’ journal January 2008
A Propionate-Inducible Expression System for Enteric Bacteria journal November 2005
Molecular analysis of the soluble butane monooxygenase from ‘Pseudomonas butanovora’ a aThe GenBank accession number for the bmoXYBZDC sequence is AY093933. journal November 2002

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60 APPLIED LIFE SCIENCES