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Title: Diffusion in biofilms respiring on electrodes

Abstract

The goal of this study was to measure spatially and temporally resolved effective diffusion coefficients (De) in biofilms respiring on electrodes. Two model electrochemically active biofilms, Geobacter sulfurreducens PCA and Shewanella oneidensis MR-1, were investigated. A novel nuclear magnetic resonance microimaging perfusion probe capable of simultaneous electrochemical and pulsed-field gradient nuclear magnetic resonance (PFG-NMR) techniques was used. PFG-NMR allowed for noninvasive, nondestructive, high spatial resolution in situ De measurements in living biofilms respiring on electrodes. The electrodes were polarized so that they would act as the sole terminal electron acceptor for microbial metabolism. We present our results as both two-dimensional De heat maps and surface-averaged relative effective diffusion coefficient (Drs) depth profiles. We found that (1) Drs decreases with depth in G. sulfurreducens biofilms, following a sigmoid shape; (2) Drs at a given location decreases with G. sulfurreducens biofilm age; (3) average De and Drs profiles in G. sulfurreducens biofilms are lower than those in S. oneidensis biofilms—the G. sulfurreducens biofilms studied here were on average 10 times denser than the S. oneidensis biofilms; and (4) halting the respiration of a G. sulfurreducens biofilm decreases the De values. Density, reflected by De, plays a major role in the extracellular electronmore » transfer strategies of electrochemically active biofilms.« less

Authors:
 [1];  [1];  [2];  [1]
  1. Washington State Univ., Pullman, WA (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1094935
Report Number(s):
PNNL-SA-94190
Journal ID: ISSN ‎1754-5692; 34744; 47495; 400403309
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Energy and Environmental Science
Additional Journal Information:
Journal Volume: 6; Journal Issue: 2; Journal ID: ISSN ‎1754-5692
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; SHEWANELLA-ONEIDENSIS MR-1; MICROBIAL FUEL-CELLS; GEOBACTER-SULFURREDUCENS BIOFILMS; WASTE-WATER TREATMENT; ELECTRICAL-CONDUCTIVITY; ELECTROACTIVE BIOFILMS; CONFOCAL MICROSCOPY; BACTERIAL NANOWIRES; MAGNETIC-RESONANCE; DESALINATION CELL; Environmental Molecular Sciences Laboratory

Citation Formats

Renslow, Ryan S., Babauta, Jerome T., Majors, Paul D., and Beyenal, Haluk. Diffusion in biofilms respiring on electrodes. United States: N. p., 2012. Web. doi:10.1039/C2EE23394K.
Renslow, Ryan S., Babauta, Jerome T., Majors, Paul D., & Beyenal, Haluk. Diffusion in biofilms respiring on electrodes. United States. doi:10.1039/C2EE23394K.
Renslow, Ryan S., Babauta, Jerome T., Majors, Paul D., and Beyenal, Haluk. Thu . "Diffusion in biofilms respiring on electrodes". United States. doi:10.1039/C2EE23394K.
@article{osti_1094935,
title = {Diffusion in biofilms respiring on electrodes},
author = {Renslow, Ryan S. and Babauta, Jerome T. and Majors, Paul D. and Beyenal, Haluk},
abstractNote = {The goal of this study was to measure spatially and temporally resolved effective diffusion coefficients (De) in biofilms respiring on electrodes. Two model electrochemically active biofilms, Geobacter sulfurreducens PCA and Shewanella oneidensis MR-1, were investigated. A novel nuclear magnetic resonance microimaging perfusion probe capable of simultaneous electrochemical and pulsed-field gradient nuclear magnetic resonance (PFG-NMR) techniques was used. PFG-NMR allowed for noninvasive, nondestructive, high spatial resolution in situ De measurements in living biofilms respiring on electrodes. The electrodes were polarized so that they would act as the sole terminal electron acceptor for microbial metabolism. We present our results as both two-dimensional De heat maps and surface-averaged relative effective diffusion coefficient (Drs) depth profiles. We found that (1) Drs decreases with depth in G. sulfurreducens biofilms, following a sigmoid shape; (2) Drs at a given location decreases with G. sulfurreducens biofilm age; (3) average De and Drs profiles in G. sulfurreducens biofilms are lower than those in S. oneidensis biofilms—the G. sulfurreducens biofilms studied here were on average 10 times denser than the S. oneidensis biofilms; and (4) halting the respiration of a G. sulfurreducens biofilm decreases the De values. Density, reflected by De, plays a major role in the extracellular electron transfer strategies of electrochemically active biofilms.},
doi = {10.1039/C2EE23394K},
journal = {Energy and Environmental Science},
issn = {‎1754-5692},
number = 2,
volume = 6,
place = {United States},
year = {2012},
month = {11}
}