Feedback-Controlled LED Photobioreactor for Photophysiological Studies of Cyanobacteria
Abstract
A custom photobioreactor (PBR) was designed to enable automatic light adjustments using computerized feedback control. A black anodized aluminum enclosure, constructed to surround the borosilicate reactor vessel, prevents the transmission of ambient light and serves as a mount for arrays of light-emitting diodes (LEDs). The high-output LEDs provide narrow-band light of either 630 or 680 nm for preferential excitation of the cyanobacterial light-harvesting pigments, phycobilin or chlorophyll a, respectively. Custom developed software BioLume provides automatic control of optical properties and a computer feedback loop can automatically adjust the incident irradiance as necessary to maintain a fixed transmitted light through the culture, based on user-determined set points. This feedback control serves to compensate for culture dynamics which have optical effects, (e.g., changing cell density, pigment adaptations) and thus can determine the appropriate light conditions for physiological comparisons or to cultivate light-sensitive strains, without prior analyses. The LED PBR may also be controlled as a turbidostat, using a feedback loop to continuously adjust the rate of media-dilution based on the transmitted light measurements, with a fast and precise response. This cultivation system gains further merit as a high-performance analytical device, using non-invasive tools (e.g., dissolved gas sensors, online mass spectrometry) to automatemore »
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1094927
- Report Number(s):
- PNNL-SA-91362
KP1601010
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Journal Article
- Journal Name:
- Bioresource Technology, 134:127-133
- Additional Journal Information:
- Journal Name: Bioresource Technology, 134:127-133
- Country of Publication:
- United States
- Language:
- English
- Subject:
- photobioreactor; light emitting diode; controlled cultivation; turbidostat; cyanobacteria; feedback control
Citation Formats
Melnicki, Matthew R., Pinchuk, Grigoriy E., Hill, Eric A., Kucek, Leo A., Stolyar, Sergey, Fredrickson, Jim K., Konopka, Allan, and Beliaev, Alex S. Feedback-Controlled LED Photobioreactor for Photophysiological Studies of Cyanobacteria. United States: N. p., 2013.
Web. doi:10.1016/j.biortech.2013.01.079.
Melnicki, Matthew R., Pinchuk, Grigoriy E., Hill, Eric A., Kucek, Leo A., Stolyar, Sergey, Fredrickson, Jim K., Konopka, Allan, & Beliaev, Alex S. Feedback-Controlled LED Photobioreactor for Photophysiological Studies of Cyanobacteria. United States. https://doi.org/10.1016/j.biortech.2013.01.079
Melnicki, Matthew R., Pinchuk, Grigoriy E., Hill, Eric A., Kucek, Leo A., Stolyar, Sergey, Fredrickson, Jim K., Konopka, Allan, and Beliaev, Alex S. 2013.
"Feedback-Controlled LED Photobioreactor for Photophysiological Studies of Cyanobacteria". United States. https://doi.org/10.1016/j.biortech.2013.01.079.
@article{osti_1094927,
title = {Feedback-Controlled LED Photobioreactor for Photophysiological Studies of Cyanobacteria},
author = {Melnicki, Matthew R. and Pinchuk, Grigoriy E. and Hill, Eric A. and Kucek, Leo A. and Stolyar, Sergey and Fredrickson, Jim K. and Konopka, Allan and Beliaev, Alex S.},
abstractNote = {A custom photobioreactor (PBR) was designed to enable automatic light adjustments using computerized feedback control. A black anodized aluminum enclosure, constructed to surround the borosilicate reactor vessel, prevents the transmission of ambient light and serves as a mount for arrays of light-emitting diodes (LEDs). The high-output LEDs provide narrow-band light of either 630 or 680 nm for preferential excitation of the cyanobacterial light-harvesting pigments, phycobilin or chlorophyll a, respectively. Custom developed software BioLume provides automatic control of optical properties and a computer feedback loop can automatically adjust the incident irradiance as necessary to maintain a fixed transmitted light through the culture, based on user-determined set points. This feedback control serves to compensate for culture dynamics which have optical effects, (e.g., changing cell density, pigment adaptations) and thus can determine the appropriate light conditions for physiological comparisons or to cultivate light-sensitive strains, without prior analyses. The LED PBR may also be controlled as a turbidostat, using a feedback loop to continuously adjust the rate of media-dilution based on the transmitted light measurements, with a fast and precise response. This cultivation system gains further merit as a high-performance analytical device, using non-invasive tools (e.g., dissolved gas sensors, online mass spectrometry) to automate real-time measurements, thus permitting unsupervised experiments to search for optimal growth conditions, to monitor physiological responses to perturbations, as well as to quantitate photophysiological parameters using an in situ light-saturation response routine.},
doi = {10.1016/j.biortech.2013.01.079},
url = {https://www.osti.gov/biblio/1094927},
journal = {Bioresource Technology, 134:127-133},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 09 00:00:00 EDT 2013},
month = {Tue Apr 09 00:00:00 EDT 2013}
}