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Title: Identification of soybean proteins from a single cell type: The root hair

Abstract

Root hairs are a terminally differentiated single cell type, mainly involved in water and nutrient uptake from the soil. The soybean root hair cell represents an excellent model for the study of single cell systems biology. In this study, we identified 5702 proteins, with at least two peptides, from soybean root hairs using an accurate mass and time tag approach, establishing the most comprehensive proteome reference map of this single cell type. We also showed that trypsin is the most appropriate enzyme for soybean proteomic studies by performing an in silico digestion of the soybean proteome database using different proteases. Although the majority of proteins identified in this study are involved in basal metabolism, the function of others are more related to root hair formation/function and include proteins involved in nutrient uptake (transporters) or vesicular trafficking (cytoskeleton and RAB proteins). Interestingly, some of these proteins appear to be specifically expressed in root hairs and constitute very good candidates for further studies to elucidate unique features of this single cell model.

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1092015
Report Number(s):
PNNL-SA-86320
44703; 30198; 24795; KP1704020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Proteomics, 12(22):3365-3373
Additional Journal Information:
Journal Name: Proteomics, 12(22):3365-3373
Country of Publication:
United States
Language:
English
Subject:
proteomics; soybean; roothair; proteins; mass spectrometry; Environmental Molecular Sciences Laboratory

Citation Formats

Brechenmacher, Laurent, Nguyen, Tran H., Hixson, Kim K., Libault, Marc, Aldrich, Joshua T., Pasa-Tolic, Ljiljana, and Stacey, Gary. Identification of soybean proteins from a single cell type: The root hair. United States: N. p., 2012. Web. doi:10.1002/pmic.201200160.
Brechenmacher, Laurent, Nguyen, Tran H., Hixson, Kim K., Libault, Marc, Aldrich, Joshua T., Pasa-Tolic, Ljiljana, & Stacey, Gary. Identification of soybean proteins from a single cell type: The root hair. United States. doi:10.1002/pmic.201200160.
Brechenmacher, Laurent, Nguyen, Tran H., Hixson, Kim K., Libault, Marc, Aldrich, Joshua T., Pasa-Tolic, Ljiljana, and Stacey, Gary. Thu . "Identification of soybean proteins from a single cell type: The root hair". United States. doi:10.1002/pmic.201200160.
@article{osti_1092015,
title = {Identification of soybean proteins from a single cell type: The root hair},
author = {Brechenmacher, Laurent and Nguyen, Tran H. and Hixson, Kim K. and Libault, Marc and Aldrich, Joshua T. and Pasa-Tolic, Ljiljana and Stacey, Gary},
abstractNote = {Root hairs are a terminally differentiated single cell type, mainly involved in water and nutrient uptake from the soil. The soybean root hair cell represents an excellent model for the study of single cell systems biology. In this study, we identified 5702 proteins, with at least two peptides, from soybean root hairs using an accurate mass and time tag approach, establishing the most comprehensive proteome reference map of this single cell type. We also showed that trypsin is the most appropriate enzyme for soybean proteomic studies by performing an in silico digestion of the soybean proteome database using different proteases. Although the majority of proteins identified in this study are involved in basal metabolism, the function of others are more related to root hair formation/function and include proteins involved in nutrient uptake (transporters) or vesicular trafficking (cytoskeleton and RAB proteins). Interestingly, some of these proteins appear to be specifically expressed in root hairs and constitute very good candidates for further studies to elucidate unique features of this single cell model.},
doi = {10.1002/pmic.201200160},
journal = {Proteomics, 12(22):3365-3373},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {11}
}