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Title: Metaproteomics reveals abundant transposase expression in mutualistic endosymbionts

Abstract

Transposases, enzymes that catalyze the movement of mobile genetic elements, are the most abundant genes in nature. While many bacteria encode an abundance of transposases in their genomes, the current paradigm is that transposase gene expression is tightly regulated and generally low due to its severe mutagenic effects. In the current study, we detected the highest number of transposase proteins ever reported in bacteria, in symbionts of the gutless marine worm Olavius algarvensis using metaproteomics. At least 26 different transposases from 12 different families were detected and genomic and proteomic analyses suggest many of these are active. This high expression of transposases indicates that the mechanisms for their tight regulation have been disabled or destroyed. Based on recent studies on other symbionts and pathogens that showed high transposase transcription, we speculate that abundant transposase expression might be common in symbionts and pathogens.

Authors:
 [1];  [2];  [2];  [2];  [1]
  1. Max Planck Institute for Marine Microbiology
  2. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1088125
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
mBio (Online)
Additional Journal Information:
Journal Volume: 4; Journal Issue: 3; Journal ID: ISSN 2150-7511
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English

Citation Formats

Kleiner, Manuel, Young, Jacque C, Shah, Manesh B, Verberkmoes, Nathan C, and Dubilier, Nicole. Metaproteomics reveals abundant transposase expression in mutualistic endosymbionts. United States: N. p., 2013. Web. doi:10.1128/mBio.00223-13.
Kleiner, Manuel, Young, Jacque C, Shah, Manesh B, Verberkmoes, Nathan C, & Dubilier, Nicole. Metaproteomics reveals abundant transposase expression in mutualistic endosymbionts. United States. doi:10.1128/mBio.00223-13.
Kleiner, Manuel, Young, Jacque C, Shah, Manesh B, Verberkmoes, Nathan C, and Dubilier, Nicole. Tue . "Metaproteomics reveals abundant transposase expression in mutualistic endosymbionts". United States. doi:10.1128/mBio.00223-13.
@article{osti_1088125,
title = {Metaproteomics reveals abundant transposase expression in mutualistic endosymbionts},
author = {Kleiner, Manuel and Young, Jacque C and Shah, Manesh B and Verberkmoes, Nathan C and Dubilier, Nicole},
abstractNote = {Transposases, enzymes that catalyze the movement of mobile genetic elements, are the most abundant genes in nature. While many bacteria encode an abundance of transposases in their genomes, the current paradigm is that transposase gene expression is tightly regulated and generally low due to its severe mutagenic effects. In the current study, we detected the highest number of transposase proteins ever reported in bacteria, in symbionts of the gutless marine worm Olavius algarvensis using metaproteomics. At least 26 different transposases from 12 different families were detected and genomic and proteomic analyses suggest many of these are active. This high expression of transposases indicates that the mechanisms for their tight regulation have been disabled or destroyed. Based on recent studies on other symbionts and pathogens that showed high transposase transcription, we speculate that abundant transposase expression might be common in symbionts and pathogens.},
doi = {10.1128/mBio.00223-13},
journal = {mBio (Online)},
issn = {2150-7511},
number = 3,
volume = 4,
place = {United States},
year = {2013},
month = {1}
}