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Title: Fermentation and Hydrogen Metabolism Affect Uranium Reduction by Clostridia

Abstract

Previously, it has been shown that not only is uranium reduction under fermentation condition common among clostridia species, but also the strains differed in the extent of their capability and the pH of the culture significantly affected uranium(VI) reduction. In this study, using HPLC and GC techniques, metabolic properties of those clostridial strains active in uranium reduction under fermentation conditions have been characterized and their effects on capability variance of uranium reduction discussed. Then, the relationship between hydrogen metabolism and uranium reduction has been further explored and the important role played by hydrogenase in uranium(VI) and iron(III) reduction by clostridia demonstrated. When hydrogen was provided as the headspace gas, uranium(VI) reduction occurred in the presence of whole cells of clostridia. This is in contrast to that of nitrogen as the headspace gas. Without clostridia cells, hydrogen alone could not result in uranium(VI) reduction. In alignment with this observation, it was also found that either copper(II) addition or iron depletion in the medium could compromise uranium reduction by clostridia. In the end, a comprehensive model was proposed to explain uranium reduction by clostridia and its relationship to the overall metabolism especially hydrogen (H 2) production.

Authors:
 [1];  [2]
  1. Center for Biosignatures Discovery Automation, Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA
  2. Division of Advanced Nuclear Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea; Environmental Sciences Department, Brookhaven National Laboratory, Upton, NY 11973, USA
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1087522
Report Number(s):
BNL-100701-2013-JA
Journal ID: ISSN 2090-9403; R&D Project: 10198; KP1702010
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Journal Article
Journal Name:
ISRN Biotechnology
Additional Journal Information:
Journal Volume: 2013; Journal Issue: none; Journal ID: ISSN 2090-9403
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES

Citation Formats

Gao, Weimin, and Francis, Arokiasamy J. Fermentation and Hydrogen Metabolism Affect Uranium Reduction by Clostridia. United States: N. p., 2013. Web. doi:10.5402/2013/657160.
Gao, Weimin, & Francis, Arokiasamy J. Fermentation and Hydrogen Metabolism Affect Uranium Reduction by Clostridia. United States. doi:10.5402/2013/657160.
Gao, Weimin, and Francis, Arokiasamy J. Tue . "Fermentation and Hydrogen Metabolism Affect Uranium Reduction by Clostridia". United States. doi:10.5402/2013/657160.
@article{osti_1087522,
title = {Fermentation and Hydrogen Metabolism Affect Uranium Reduction by Clostridia},
author = {Gao, Weimin and Francis, Arokiasamy J.},
abstractNote = {Previously, it has been shown that not only is uranium reduction under fermentation condition common among clostridia species, but also the strains differed in the extent of their capability and the pH of the culture significantly affected uranium(VI) reduction. In this study, using HPLC and GC techniques, metabolic properties of those clostridial strains active in uranium reduction under fermentation conditions have been characterized and their effects on capability variance of uranium reduction discussed. Then, the relationship between hydrogen metabolism and uranium reduction has been further explored and the important role played by hydrogenase in uranium(VI) and iron(III) reduction by clostridia demonstrated. When hydrogen was provided as the headspace gas, uranium(VI) reduction occurred in the presence of whole cells of clostridia. This is in contrast to that of nitrogen as the headspace gas. Without clostridia cells, hydrogen alone could not result in uranium(VI) reduction. In alignment with this observation, it was also found that either copper(II) addition or iron depletion in the medium could compromise uranium reduction by clostridia. In the end, a comprehensive model was proposed to explain uranium reduction by clostridia and its relationship to the overall metabolism especially hydrogen (H2) production.},
doi = {10.5402/2013/657160},
journal = {ISRN Biotechnology},
issn = {2090-9403},
number = none,
volume = 2013,
place = {United States},
year = {2013},
month = {1}
}

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