skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions

Abstract

A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
LLNL (Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States))
Sponsoring Org.:
USDOE
OSTI Identifier:
1086952
Patent Number(s):
8,470,537
Application Number:
12/959,789
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA) LLNL
DOE Contract Number:
AC52-07NA27344
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gardner, Shea N, Mariella, Jr., Raymond P, Christian, Allen T, Young, Jennifer A, and Clague, David S. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions. United States: N. p., 2013. Web.
Gardner, Shea N, Mariella, Jr., Raymond P, Christian, Allen T, Young, Jennifer A, & Clague, David S. Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions. United States.
Gardner, Shea N, Mariella, Jr., Raymond P, Christian, Allen T, Young, Jennifer A, and Clague, David S. Tue . "Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions". United States. doi:. https://www.osti.gov/servlets/purl/1086952.
@article{osti_1086952,
title = {Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions},
author = {Gardner, Shea N and Mariella, Jr., Raymond P and Christian, Allen T and Young, Jennifer A and Clague, David S},
abstractNote = {A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 25 00:00:00 EDT 2013},
month = {Tue Jun 25 00:00:00 EDT 2013}
}

Patent:

Save / Share: