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Title: Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins

Abstract

The invention provides an in vitro method for producing proteins, membrane proteins, membrane-associated proteins, and soluble proteins that interact with membrane-associated proteins for assembly into an oligomeric complex or that require association with a membrane for proper folding. The method comprises, supplying intracytoplasmic membranes from organisms; modifying protein composition of intracytoplasmic membranes from organism by modifying DNA to delete genes encoding functions of the organism not associated with the formation of the intracytoplasmic membranes; generating appropriate DNA or RNA templates that encode the target protein; and mixing the intracytoplasmic membranes with the template and a transcription/translation-competent cellular extract to cause simultaneous production of the membrane proteins and encapsulation of the membrane proteins within the intracytoplasmic membranes.

Inventors:
;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1084360
Patent Number(s):
8,455,231
Application Number:
12/702,167
Assignee:
Uchicago Argonne, LLC (Chicago, IL) CHO
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Laible, Philip D, and Hanson, Deborah K. Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins. United States: N. p., 2013. Web.
Laible, Philip D, & Hanson, Deborah K. Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins. United States.
Laible, Philip D, and Hanson, Deborah K. Tue . "Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins". United States. https://www.osti.gov/servlets/purl/1084360.
@article{osti_1084360,
title = {Cell-free system for synthesizing membrane proteins cell free method for synthesizing membrane proteins},
author = {Laible, Philip D and Hanson, Deborah K},
abstractNote = {The invention provides an in vitro method for producing proteins, membrane proteins, membrane-associated proteins, and soluble proteins that interact with membrane-associated proteins for assembly into an oligomeric complex or that require association with a membrane for proper folding. The method comprises, supplying intracytoplasmic membranes from organisms; modifying protein composition of intracytoplasmic membranes from organism by modifying DNA to delete genes encoding functions of the organism not associated with the formation of the intracytoplasmic membranes; generating appropriate DNA or RNA templates that encode the target protein; and mixing the intracytoplasmic membranes with the template and a transcription/translation-competent cellular extract to cause simultaneous production of the membrane proteins and encapsulation of the membrane proteins within the intracytoplasmic membranes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {6}
}

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