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Title: Method for in vitro recombination

Abstract

The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.

Inventors:
;
Publication Date:
Research Org.:
Synthetic Genomics, Inc. (La Jolla, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1084234
Patent Number(s):
8,435,736
Application Number:
12/820,861
Assignee:
Synthetic Genomics, Inc. (La Jolla, CA) DOESC
DOE Contract Number:  
FG02-02ER63453
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gibson, Daniel Glenn, and Smith, Hamilton O. Method for in vitro recombination. United States: N. p., 2013. Web.
Gibson, Daniel Glenn, & Smith, Hamilton O. Method for in vitro recombination. United States.
Gibson, Daniel Glenn, and Smith, Hamilton O. Tue . "Method for in vitro recombination". United States. https://www.osti.gov/servlets/purl/1084234.
@article{osti_1084234,
title = {Method for in vitro recombination},
author = {Gibson, Daniel Glenn and Smith, Hamilton O},
abstractNote = {The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {5}
}

Patent:

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