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Title: Cellulases, nucleic acids encoding them and methods for making and using them

Patent ·
OSTI ID:1083313

This invention relates to molecular and cellular biology and biochemistry. In one aspect, the invention provides polypeptides having cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or .beta.-glucosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides cellulase activity, e.g., endoglucanase, cellobiohydrolase, mannanase and/or .beta.-glucosidase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts.

Research Organization:
BP Corporation North America (Houston, TX); Verenium Corporation (San Diego, CA)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-02ER83395
Assignee:
BP Corporation North America (Houston, TX) Verenium Corporation (San Diego, CA);
Patent Number(s):
8,426,184
Application Number:
11/908,672
OSTI ID:
1083313
Country of Publication:
United States
Language:
English

References (79)

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design journal August 2005
Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis. journal July 1988
.beta.-glucosidase coding sequences and protein from orpinomyces PC-2 patent February 2001
Thermal tolerant exoglucanase from Acidothermus cellulolyticus patent July 2008
Treatment of fabrics patent June 2000
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects journal October 1993
Cloning and amplification of the .beta.-glucosidase gene of Trichoderma reesei patent February 2000
Trends in biochemistry and enzymology of cellulose degradation journal November 1990
Developments in Directed Evolution for Improving Enzyme Functions journal August 2007
Structural and sequence-based classification of glycoside hydrolases journal October 1997
Identification of a functionally important carboxyl group in cellobiohydrolase I from Trichoderma reesei: A chemical modification study journal January 1989
Mobile Bioreactor and Biogenerator patent November 1998
Prediction of protein function from protein sequence and structure journal January 1999
Site-Directed Mutagenesis and Expression of the Soluble Form of the Family IIIa Cellulose Binding Domain from the Cellulosomal Scaffolding Protein of Clostridium cellulovorans journal September 2005
Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems journal November 2004
Production of soft paper products from old newspaper patent December 1996
High-Resolution Capillary Gel Electrophoresis of Reducing Oligosaccharides Labeled with 1-Aminopyrene-3,6,8-trisulfonate journal January 1996
Nucleotide sequence of thecelA gene encoding a cellodextrinase ofRuminococcus flavefaciens FD-1 journal July 1990
Process for the production of organic products from diverse biomass sources patent June 2002
Phenolic acid esterases, coding sequences and methods patent August 2003
Preparation of low alcohol beer patent November 1988
Treatment of animal waste patent September 1999
Crystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 Å resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediate journal October 1998
Cellulase assay methods: a review journal October 1988
Roles of the Catalytic Domain and Two Cellulose Binding Domains of Thermomonospora fusca E4 in Cellulose Hydrolysis journal January 1998
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002
Cellulase preparation comprising an endoglucanase enzyme patent July 2002
Protein having .alpha.-glucosidase activity, DNA having genetic information thereof, and production of .alpha.-glucosidase patent August 1998
Coniferin beta-glucosidase cDNA for modifying lignin content in plants patent October 1999
Ethanol production with dilute acid hydrolysis using partially dried lignocellulosics patent December 2003
Hemicellulose bioconversion journal May 2003
A new type of cohesin domain that specifically binds the dockerin domain of the Clostridium thermocellum cellulosome-integrating protein CipA. journal June 1996
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001
Structure of the beta-glucosidase gene bglA of Clostridium thermocellum. Sequence analysis reveals a superfamily of cellulases and beta-glycosidases including human lactase/phlorizin hydrolase journal September 1991
Carbohydrate-binding modules: fine-tuning polysaccharide recognition journal September 2004
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids journal November 1998
Endoglucanases and cellulase preparations containing the same patent July 2005
Synergistic Effects of Cellulosomal Xylanase and Cellulases from Clostridium cellulovorans on Plant Cell Wall Degradation journal March 2003
Protein having cellulase activities and process for producing the same patent October 2000
Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca journal October 1997
The Topology of the Substrate Binding Clefts of Glycosyl Hydrolase Family 10 Xylanases Are Not Conserved journal November 1998
Basic local alignment search tool journal October 1990
Polypeptide having cellobiohydrolase activity patent May 2003
Epoxide hydrolases, nucleic acids encoding them and methods of making and using them patent December 2005
A carbohydrate binding module as a diversity-carrying scaffold journal February 2004
Pretreatment process for conversion of cellulose to fuel ethanol patent July 2000
Methods for measuring cellulase activities journal January 1988
Nucleotide sequence of the Ruminococcus albus SY3 endoglucanase genes ce1A and ce1B journal September 1990
Prehydrolysis of lignocellulose patent January 1998
Comparative amino acid sequence analysis of Thermotoga maritima β-glucosidase (BglA) deduced from the nucleotide sequence of the gene indicates distant relationship between β-glucosidases of the BGA family and other families of β-1,4-glycosyl hydrolases journal January 1994
Engineered xyloglucan specificity in a carbohydrate-binding module journal July 2006
Cloning and DNA Sequencing of the Genes EncodingClostridium josui Scaffolding Protein CipA and Cellulase CelD and Identification of Their Gene Products as Major Components of the Cellulosome journal January 1998
Glucose and cellobiose tolerant .beta.-glucosidase from Candida peltata patent May 1998
Kinetic Measurements of Cellulase Activity on Insoluble Substrates Using Disodium 2,2' Bicinchoninate journal September 1998
Detection and quantitation of cellulase by Congo red staining of substrates in a cup-plate diffusion assay journal February 1986
Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice journal July 2003
Expression cloning in filamentous fungi patent May 2007
Method of improving pulp freeness using cellulase and pectinase enzymes patent May 2000
.beta.-glucosidase from filamentous fungi, and uses thereof patent July 2000
Expression of microbial proteins in plants for production of plants with improved properties patent-application December 2003
Hybrid enzymes patent October 2006
A cellulase assay coupled to cellobiose dehydrogenase journal June 1985
Biochemical characterization and mode of action of a thermostable endoglucanase purified from Thermoascus aurantiacus journal August 2002
Estimation of cellulase activity using a glucose-oxidase-Cu(II) reducing assay for glucose journal December 1991
Process for digesting cellulose containing solid wastes patent January 1998
Microbial hemicellulases journal June 2003
Ethanol fermentation from biomass resources: current state and prospects journal December 2005
A newly described cellulosomal cellobiohydrolase, CelO, from Clostridium thermocellum: investigation of the exo-mode of hydrolysis, and binding capacity to crystalline cellulose journal January 2002
Consolidated bioprocessing of cellulosic biomass: an update journal October 2005
Characterization and subcellular localization of the Clostridium thermocellum scaffoldin dockerin binding protein SdbA. journal January 1997
Sensitive assay for cellulase and dextranase journal June 1976
Dilute acid/metal salt hydrolysis of lignocellulosics patent July 2002
Methods for deinking and decolorizing printed paper patent June 2001
Protein tolerance to random amino acid change journal June 2004
Characterization and Disruption of A Gene in the Maize Pathogen Cochliobolus carbonum Encoding a Cellulase Lacking a Cellulose Binding Domain and Hinge Region journal January 1995
Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 Å resolution journal January 1999
Cello-Oligosaccharide Hydrolysis by Cellobiohydrolase II from Trichoderma Reesei. Association and Rate Constants Derived from an Analysis of Progress Curves journal September 1996
Method for using a vegetable biomass and a screw press to carry out said method patent June 2001

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