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Title: Materials and methods for efficient lactic acid production

Abstract

The present invention provides derivatives of Escherichia coli constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1083310
Patent Number(s):
8,426,191
Application Number:
12/632,041
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
DOE Contract Number:  
FG02-96ER20222; FG36-04GO14019
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Zhou, Shengde, Ingram, Lonnie O'Neal, Shanmugam, Keelnatham T, Yomano, Lorraine, Grabar, Tammy B, and Moore, Jonathan C. Materials and methods for efficient lactic acid production. United States: N. p., 2013. Web.
Zhou, Shengde, Ingram, Lonnie O'Neal, Shanmugam, Keelnatham T, Yomano, Lorraine, Grabar, Tammy B, & Moore, Jonathan C. Materials and methods for efficient lactic acid production. United States.
Zhou, Shengde, Ingram, Lonnie O'Neal, Shanmugam, Keelnatham T, Yomano, Lorraine, Grabar, Tammy B, and Moore, Jonathan C. 2013. "Materials and methods for efficient lactic acid production". United States. https://www.osti.gov/servlets/purl/1083310.
@article{osti_1083310,
title = {Materials and methods for efficient lactic acid production},
author = {Zhou, Shengde and Ingram, Lonnie O'Neal and Shanmugam, Keelnatham T and Yomano, Lorraine and Grabar, Tammy B and Moore, Jonathan C},
abstractNote = {The present invention provides derivatives of Escherichia coli constructed for the production of lactic acid. The transformed E. coli of the invention are prepared by deleting the genes that encode competing pathways followed by a growth-based selection for mutants with improved performance. These transformed E. coli are useful for providing an increased supply of lactic acid for use in food and industrial applications.},
doi = {},
url = {https://www.osti.gov/biblio/1083310}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {4}
}

Works referenced in this record:

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Fermentation of 10% (w/v) Sugar to D(−)-Lactate by Engineered Escherichia coli B
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Functional overlap between RecA and MgsA (RarA) in the rescue of stalled replication forks in Escherichia coli
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Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
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Production of Optically Pure D-Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110
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Production of l-alanine by metabolically engineered Escherichia coli
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Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli
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Recombinant Escherichia coli engineered for production of L-lactic acid from hexose and pentose sugars
journal, October 2001