skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods

Abstract

A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.

Inventors:
;
Publication Date:
Research Org.:
PNNL (Pacific Northwest National Laboratory (PNNL), Richland, WA (United States))
Sponsoring Org.:
USDOE
OSTI Identifier:
1082850
Patent Number(s):
8,354,499
Application Number:
12/773,707
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
DOE Contract Number:  
AC0676RLO1830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Mayer-Cumblidge, M. Uljana, and Cao, Haishi. Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods. United States: N. p., 2013. Web.
Mayer-Cumblidge, M. Uljana, & Cao, Haishi. Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods. United States.
Mayer-Cumblidge, M. Uljana, and Cao, Haishi. Tue . "Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods". United States. https://www.osti.gov/servlets/purl/1082850.
@article{osti_1082850,
title = {Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods},
author = {Mayer-Cumblidge, M. Uljana and Cao, Haishi},
abstractNote = {A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {1}
}

Patent:

Save / Share: