Cyanine-based probe\tag-peptide pair fluorescence protein imaging and fluorescence protein imaging methods
A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl.sub.3. The As is liganded to ethanedithiol to produce a probe having a second formula. A method of labeling a peptide includes providing a peptide comprising a tag sequence and contacting the peptide with a biarsenical molecular probe. A complex is formed comprising the tag sequence and the molecular probe. A method of studying a peptide includes providing a mixture containing a peptide comprising a peptide tag sequence, adding a biarsenical probe to the mixture, and monitoring the fluorescence of the mixture.
- Research Organization:
- PNNL (Pacific Northwest National Laboratory (PNNL), Richland, WA (United States))
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC06-76RL01830
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Number(s):
- 8,354,499
- Application Number:
- 12/773,707
- OSTI ID:
- 1082850
- Country of Publication:
- United States
- Language:
- English
Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
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journal | July 1998 |
New Biarsenical Ligands and Tetracysteine Motifs for Protein Labeling in Vitro and in Vivo: Synthesis and Biological Applications
|
journal | May 2002 |
A Fusion of Disciplines: Chemical Approaches to Exploit Fusion Proteins for Functional Genomics
|
journal | September 2003 |
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