skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Characterization of Residual Medium Peptides from Yersinia pestis Cultures

Abstract

Using a range of common microbial medium formulations (TSB, BHI, LB, and G-media), two attenuated strains of Y. pestis (KIM D27 (pgm-) and KIMD1 lcr-) were cultivated in triplicate. These cellular suspensions were used to develop a method of extracting residual medium peptides from the final microbial preparation to assess their relative abundance and identity. Across the conditions examined, which included additional cellular washing and different forms of microbial inactivation, residual medium peptides were detected. Despite the range of growth medium sources used and the associated manufacturing processes used in their production, a high degree of peptide similarity was observed for a given medium recipe. These results demonstrate that residual medium peptides are retained using traditional microbial cultivation techniques and may be used to inform forensic investigations with respect to production deduction.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1082586
Report Number(s):
PNNL-SA-91391
400904120
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry, 85(8):3933-3939
Additional Journal Information:
Journal Name: Analytical Chemistry, 85(8):3933-3939
Country of Publication:
United States
Language:
English
Subject:
Microbial Forensics, Proteomics, Peptidomic, Mass Spectrometry

Citation Formats

Clowers, Brian H., Wunschel, David S., Kreuzer, Helen W., Engelmann, Heather E., Valentine, Nancy B., and Wahl, Karen L. Characterization of Residual Medium Peptides from Yersinia pestis Cultures. United States: N. p., 2013. Web. doi:10.1021/ac3034272.
Clowers, Brian H., Wunschel, David S., Kreuzer, Helen W., Engelmann, Heather E., Valentine, Nancy B., & Wahl, Karen L. Characterization of Residual Medium Peptides from Yersinia pestis Cultures. United States. doi:10.1021/ac3034272.
Clowers, Brian H., Wunschel, David S., Kreuzer, Helen W., Engelmann, Heather E., Valentine, Nancy B., and Wahl, Karen L. Wed . "Characterization of Residual Medium Peptides from Yersinia pestis Cultures". United States. doi:10.1021/ac3034272.
@article{osti_1082586,
title = {Characterization of Residual Medium Peptides from Yersinia pestis Cultures},
author = {Clowers, Brian H. and Wunschel, David S. and Kreuzer, Helen W. and Engelmann, Heather E. and Valentine, Nancy B. and Wahl, Karen L.},
abstractNote = {Using a range of common microbial medium formulations (TSB, BHI, LB, and G-media), two attenuated strains of Y. pestis (KIM D27 (pgm-) and KIMD1 lcr-) were cultivated in triplicate. These cellular suspensions were used to develop a method of extracting residual medium peptides from the final microbial preparation to assess their relative abundance and identity. Across the conditions examined, which included additional cellular washing and different forms of microbial inactivation, residual medium peptides were detected. Despite the range of growth medium sources used and the associated manufacturing processes used in their production, a high degree of peptide similarity was observed for a given medium recipe. These results demonstrate that residual medium peptides are retained using traditional microbial cultivation techniques and may be used to inform forensic investigations with respect to production deduction.},
doi = {10.1021/ac3034272},
journal = {Analytical Chemistry, 85(8):3933-3939},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {4}
}