skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme

Abstract

Systems and methods for combining dielectrophoresis, magnetic forces, and hydrodynamic forces to manipulate particles in channels formed on top of an electrode substrate are discussed. A magnet placed in contact under the electrode substrate while particles are flowing within the channel above the electrode substrate allows these three forces to be balanced when the system is in operation. An optical detection scheme using near-confocal microscopy for simultaneously detecting two wavelengths of light emitted from the flowing particles is also discussed.

Inventors:
; ; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1079398
Patent Number(s):
8,293,089
Application Number:
12/610,409
Assignee:
Sandia Corporation (Albuquerque, NM)
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

James, Conrad D, Galambos, Paul C, Derzon, Mark S, Graf, Darin C, Pohl, Kenneth R, and Bourdon, Chris J. Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme. United States: N. p., 2012. Web.
James, Conrad D, Galambos, Paul C, Derzon, Mark S, Graf, Darin C, Pohl, Kenneth R, & Bourdon, Chris J. Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme. United States.
James, Conrad D, Galambos, Paul C, Derzon, Mark S, Graf, Darin C, Pohl, Kenneth R, and Bourdon, Chris J. 2012. "Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme". United States. https://www.osti.gov/servlets/purl/1079398.
@article{osti_1079398,
title = {Portable dual field gradient force multichannel flow cytometer device with a dual wavelength low noise detection scheme},
author = {James, Conrad D and Galambos, Paul C and Derzon, Mark S and Graf, Darin C and Pohl, Kenneth R and Bourdon, Chris J},
abstractNote = {Systems and methods for combining dielectrophoresis, magnetic forces, and hydrodynamic forces to manipulate particles in channels formed on top of an electrode substrate are discussed. A magnet placed in contact under the electrode substrate while particles are flowing within the channel above the electrode substrate allows these three forces to be balanced when the system is in operation. An optical detection scheme using near-confocal microscopy for simultaneously detecting two wavelengths of light emitted from the flowing particles is also discussed.},
doi = {},
url = {https://www.osti.gov/biblio/1079398}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {10}
}

Works referenced in this record:

Automated methods for multiplexed pathogen detection
journal, September 2005


Biofunctional magnetic nanoparticles for protein separation and pathogen detection
journal, January 2006


Timely multithreat biological, chemical and nuclide detection: a platform, a metric, key results
journal, January 2008


Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis
journal, January 2006


Encapsulation of organic field effect transistors for flexible biomedical microimplants
journal, April 2005


Combined field-induced dielectrophoresis and phase separation for manipulating particles in microfluidics
journal, December 2003


3D focusing of nanoparticles in microfluidic channels
journal, January 2003


Vertical Focusing Device Utilizing Dielectrophoretic Force and Its Application on Microflow Cytometer
journal, December 2004


Analyzing a bioterror attack on the food supply: The case of botulinum toxin in milk
journal, June 2005


Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples.
journal, January 1997


Fast, sensitive and cost-effective detection of nerve agents in the gas phase using a portable instrument and an electrochemical biosensor
journal, May 2007


High throughput particle analysis: Combining dielectrophoretic particle focussing with confocal optical detection
journal, February 2006


Recent advancements in the gas-phase MicroChemLab
journal, June 2006


Tungsten coil atomic emission spectrometry
journal, February 2006


Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents
journal, April 2003


Monolithic surface micromachined fluidic devices for dielectrophoretic preconcentration and routing of particles
journal, August 2006


Portable remote Raman system for monitoring hydrocarbon, gas hydrates and explosives in the environment
journal, August 2005


Automated immunomagnetic separation and microarray detection of E. coli O157:H7 from poultry carcass rinse
journal, October 2001


Rapid, femtomolar bioassays in complex matrices combining microfluidics and magnetoelectronics
journal, September 2007


Portable 24-analyte surface plasmon resonance instruments for rapid, versatile biodetection
journal, April 2007


Microfluidic ELISA on non-passivated PDMS chip using magnetic bead transfer inside dual networks of channels
journal, January 2007