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Title: Method for concentration and separation of biological organisms by ultrafiltration and dielectrophoresis

Abstract

Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to "natural" contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., <10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.

Inventors:
; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1078303
Patent Number(s):
8,257,568
Application Number:
12/899,671
Assignee:
Sandia Corporation (Albuquerque, NM) SNL
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Simmons, Blake A., Hill, Vincent R., Fintschenko, Yolanda, and Cummings, Eric B. Method for concentration and separation of biological organisms by ultrafiltration and dielectrophoresis. United States: N. p., 2012. Web.
Simmons, Blake A., Hill, Vincent R., Fintschenko, Yolanda, & Cummings, Eric B. Method for concentration and separation of biological organisms by ultrafiltration and dielectrophoresis. United States.
Simmons, Blake A., Hill, Vincent R., Fintschenko, Yolanda, and Cummings, Eric B. Tue . "Method for concentration and separation of biological organisms by ultrafiltration and dielectrophoresis". United States. https://www.osti.gov/servlets/purl/1078303.
@article{osti_1078303,
title = {Method for concentration and separation of biological organisms by ultrafiltration and dielectrophoresis},
author = {Simmons, Blake A. and Hill, Vincent R. and Fintschenko, Yolanda and Cummings, Eric B.},
abstractNote = {Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to "natural" contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., <10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {9}
}

Patent:

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