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Title: Integrated Genome-Based Studies of Shewanella Ecophysiology

Abstract

We have constructed in-frame deletions of 7 of the 10 PAS-GGDEF-EAL proteins in Shewanella oneidensis MR-1. We are currently in the process of characterizing the deletion mutants under a wide range of growth conditions. In addition to characterizing growth, we will also examine the biofilm formation of the deletion mutants. In addition to the genetic analyses of the mutants, we are also interested in comparing the activities of the various PAS-GGDEF-EAL proteins. Proteins containing PAS, GGDEF and EAL amino acid sequence motifs may play an important role in regulating c-di-GMP signaling in response to environmental conditions. A genetic and biochemical analysis into the roles of these proteins is underway. PDE activity was observed for several PAS-GGDEF-EAL proteins. One of these proteins, SO0427, also demonstrates possible DGC activity in vitro. Currently, we are studying the growth, motility and biofilm formation characteristics of deletion mutants, as well as the activity of the purified proteins.

Authors:
Publication Date:
Research Org.:
Leland Stanford Junior University
Sponsoring Org.:
USDOE
OSTI Identifier:
1077855
Report Number(s):
DOE/ER/64386
37452
DOE Contract Number:  
FG02-07ER64386
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Spormann, Alfred. Integrated Genome-Based Studies of Shewanella Ecophysiology. United States: N. p., 2011. Web. doi:10.2172/1077855.
Spormann, Alfred. Integrated Genome-Based Studies of Shewanella Ecophysiology. United States. doi:10.2172/1077855.
Spormann, Alfred. Tue . "Integrated Genome-Based Studies of Shewanella Ecophysiology". United States. doi:10.2172/1077855. https://www.osti.gov/servlets/purl/1077855.
@article{osti_1077855,
title = {Integrated Genome-Based Studies of Shewanella Ecophysiology},
author = {Spormann, Alfred},
abstractNote = {We have constructed in-frame deletions of 7 of the 10 PAS-GGDEF-EAL proteins in Shewanella oneidensis MR-1. We are currently in the process of characterizing the deletion mutants under a wide range of growth conditions. In addition to characterizing growth, we will also examine the biofilm formation of the deletion mutants. In addition to the genetic analyses of the mutants, we are also interested in comparing the activities of the various PAS-GGDEF-EAL proteins. Proteins containing PAS, GGDEF and EAL amino acid sequence motifs may play an important role in regulating c-di-GMP signaling in response to environmental conditions. A genetic and biochemical analysis into the roles of these proteins is underway. PDE activity was observed for several PAS-GGDEF-EAL proteins. One of these proteins, SO0427, also demonstrates possible DGC activity in vitro. Currently, we are studying the growth, motility and biofilm formation characteristics of deletion mutants, as well as the activity of the purified proteins.},
doi = {10.2172/1077855},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {7}
}