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Title: Phospholipid Membrane Protection by Sugar Molecules during Dehydration-Insights into Molecular Mechanisms Using Scattering Techniques

Abstract

Scattering techniques have played a key role in our understanding of the structure and function of phospholipid membranes. These techniques have been applied widely to study how different molecules (e.g., cholesterol) can affect phospholipid membrane structure. However, there has been much less attention paid to the effects of molecules that remain in the aqueous phase. One important example is the role played by small solutes, particularly sugars, in protecting phospholipid membranes during drying or slow freezing. In this paper, we present new results and a general methodology, which illustrate how contrast variation small angle neutron scattering (SANS) and synchrotron-based X-ray scattering (small angle (SAXS) and wide angle (WAXS)) can be used to quantitatively understand the interactions between solutes and phospholipids. Specifically, we show the assignment of lipid phases with synchrotron SAXS and explain how SANS reveals the exclusion of sugars from the aqueous region in the particular example of hexagonal II phases formed by phospholipids.

Authors:
; ; ; ;  [1];  [2];  [2];  [2]
  1. ANSTO
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGN
OSTI Identifier:
1074266
Resource Type:
Journal Article
Journal Name:
Int. J. Mol. Sci.
Additional Journal Information:
Journal Volume: 14; Journal Issue: (4) ; 2013
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Garvey, Christopher J., Lenné, Thomas, Koster, Karen L., Kent, Ben, Bryant, Gary, USD), ANU), and RMIT). Phospholipid Membrane Protection by Sugar Molecules during Dehydration-Insights into Molecular Mechanisms Using Scattering Techniques. United States: N. p., 2014. Web. doi:10.3390/ijms14048148.
Garvey, Christopher J., Lenné, Thomas, Koster, Karen L., Kent, Ben, Bryant, Gary, USD), ANU), & RMIT). Phospholipid Membrane Protection by Sugar Molecules during Dehydration-Insights into Molecular Mechanisms Using Scattering Techniques. United States. doi:10.3390/ijms14048148.
Garvey, Christopher J., Lenné, Thomas, Koster, Karen L., Kent, Ben, Bryant, Gary, USD), ANU), and RMIT). Wed . "Phospholipid Membrane Protection by Sugar Molecules during Dehydration-Insights into Molecular Mechanisms Using Scattering Techniques". United States. doi:10.3390/ijms14048148.
@article{osti_1074266,
title = {Phospholipid Membrane Protection by Sugar Molecules during Dehydration-Insights into Molecular Mechanisms Using Scattering Techniques},
author = {Garvey, Christopher J. and Lenné, Thomas and Koster, Karen L. and Kent, Ben and Bryant, Gary and USD) and ANU) and RMIT)},
abstractNote = {Scattering techniques have played a key role in our understanding of the structure and function of phospholipid membranes. These techniques have been applied widely to study how different molecules (e.g., cholesterol) can affect phospholipid membrane structure. However, there has been much less attention paid to the effects of molecules that remain in the aqueous phase. One important example is the role played by small solutes, particularly sugars, in protecting phospholipid membranes during drying or slow freezing. In this paper, we present new results and a general methodology, which illustrate how contrast variation small angle neutron scattering (SANS) and synchrotron-based X-ray scattering (small angle (SAXS) and wide angle (WAXS)) can be used to quantitatively understand the interactions between solutes and phospholipids. Specifically, we show the assignment of lipid phases with synchrotron SAXS and explain how SANS reveals the exclusion of sugars from the aqueous region in the particular example of hexagonal II phases formed by phospholipids.},
doi = {10.3390/ijms14048148},
journal = {Int. J. Mol. Sci.},
number = (4) ; 2013,
volume = 14,
place = {United States},
year = {2014},
month = {9}
}