Multiplexed Cancer Biomarker Detection Using Quartz-based Photonic Crystal Surfaces

Huang, Cheng-Sheng; Chaudhey, Vikram; Pokhriyal, A; George, Sherine; Polans, James; Lu, Meng; Tan, Ruimin; Zangar, Richard C; Cunningham, Brian T

doi:10.1021/ac202817q

Title: Multiplexed Cancer Biomarker Detection Using Quartz-based Photonic Crystal Surfaces

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Abstract

A photonic crystal (PC) surface is demonstrated as a high-sensitivity platform for detection of a panel of 21 cancer biomarker antigens using a sandwich enzyme-linked immunosorbent assay (ELISA) microarray format. A quartz-based PC structure fabricated by nanoimprint lithography, selected for its low autofluorescence, supports two independent optical resonances that simultaneously enable enhancement of fluorescence detection of biomarkers and label-free quantification of the density of antibody capture spots. A detection instrument is demonstrated that supports fluorescence and label-free imaging modalities, with the ability to optimize the fluorescence enhancement factor on a pixel-by-pixel basis throughout the microarray using an angle-scanning approach for the excitation laser that automatically compensates for variability in surface chemistry density and capture spot density. Measurements show that the angle-scanning illumination approach reduces the coefficient of variation of replicate assays by 20–99% compared to ordinary fluorescence microscopy, thus supporting reduction in limits of detectable biomarker concentration. Using the PC resonance, biomarkers in mixed samples were detectable at the lowest concentrations tested (2.1–41 pg/mL), resulting in a three-log range of quantitative detection.

Authors:: Huang, Cheng-Sheng; Chaudhey, Vikram; Pokhriyal, A; George, Sherine; Polans, James; Lu, Meng; Tan, Ruimin; Zangar, Richard C; Cunningham, Brian T

Publication Date:: Thu Jan 17 00:00:00 EST 2013

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1073560

Report Number(s):: PNNL-SA-85890
400412000

DOE Contract Number:: AC05-76RL01830

Resource Type:: Journal Article

Journal Name:: Analytical Chemistry, 84(2):1126-1133

Additional Journal Information:: Journal Name: Analytical Chemistry, 84(2):1126-1133

Country of Publication:: United States

Language:: English

Citation Formats


                    Huang, Cheng-Sheng, Chaudhey, Vikram, Pokhriyal, A, George, Sherine, Polans, James, Lu, Meng, Tan, Ruimin, Zangar, Richard C, and Cunningham, Brian T. Multiplexed Cancer Biomarker Detection Using Quartz-based Photonic Crystal Surfaces.  United States: N. p., 2013. 
        Web.  doi:10.1021/ac202817q.

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                    Huang, Cheng-Sheng, Chaudhey, Vikram, Pokhriyal, A, George, Sherine, Polans, James, Lu, Meng, Tan, Ruimin, Zangar, Richard C, & Cunningham, Brian T. Multiplexed Cancer Biomarker Detection Using Quartz-based Photonic Crystal Surfaces.  United States.  https://doi.org/10.1021/ac202817q

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                    Huang, Cheng-Sheng, Chaudhey, Vikram, Pokhriyal, A, George, Sherine, Polans, James, Lu, Meng, Tan, Ruimin, Zangar, Richard C, and Cunningham, Brian T. 2013.  
        "Multiplexed Cancer Biomarker Detection Using Quartz-based Photonic Crystal Surfaces".  United States.  https://doi.org/10.1021/ac202817q.

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@article{osti_1073560,

  title        = {Multiplexed Cancer Biomarker Detection Using Quartz-based Photonic Crystal Surfaces},

  author       = {Huang, Cheng-Sheng and Chaudhey, Vikram and Pokhriyal, A and George, Sherine and Polans, James and Lu, Meng and Tan, Ruimin and Zangar, Richard C and Cunningham, Brian T},

  abstractNote = {A photonic crystal (PC) surface is demonstrated as a high-sensitivity platform for detection of a panel of 21 cancer biomarker antigens using a sandwich enzyme-linked immunosorbent assay (ELISA) microarray format. A quartz-based PC structure fabricated by nanoimprint lithography, selected for its low autofluorescence, supports two independent optical resonances that simultaneously enable enhancement of fluorescence detection of biomarkers and label-free quantification of the density of antibody capture spots. A detection instrument is demonstrated that supports fluorescence and label-free imaging modalities, with the ability to optimize the fluorescence enhancement factor on a pixel-by-pixel basis throughout the microarray using an angle-scanning approach for the excitation laser that automatically compensates for variability in surface chemistry density and capture spot density. Measurements show that the angle-scanning illumination approach reduces the coefficient of variation of replicate assays by 20–99% compared to ordinary fluorescence microscopy, thus supporting reduction in limits of detectable biomarker concentration. Using the PC resonance, biomarkers in mixed samples were detectable at the lowest concentrations tested (2.1–41 pg/mL), resulting in a three-log range of quantitative detection.},

  doi          = {10.1021/ac202817q},

  url          = {https://www.osti.gov/biblio/1073560},
  journal      = {Analytical Chemistry, 84(2):1126-1133},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Jan 17 00:00:00 EST 2013},

  month        = {Thu Jan 17 00:00:00 EST 2013}

}

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