Compositions and methods for the expression of selenoproteins in eukaryotic cells

Gladyshev, Vadim; Novoselov, Sergey

Title: Compositions and methods for the expression of selenoproteins in eukaryotic cells

Patent · Tue Sep 25 00:00:00 EDT 2012

OSTI ID:1067327

Gladyshev, Vadim ^[1]; Novoselov, Sergey ^[2]

Lincoln, NE
Puschino, RU

Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG07-02ID14380

Assignee:: Board of Regents of the University of Nebraska (Lincoln, NE)

Patent Number(s):: 8,273,549

Application Number:: US Patent Application 12/428,007

OSTI ID:: 1067327

Country of Publication:: United States

Language:: English

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