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Title: The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium

Abstract

The differing composition of LH2 (peripheral light-harvesting) complexes present in Rhodopseudomonas palustris 2.1.6 have been investigated when cells are grown under progressively decreasing light intensity. Analysis of the absorption spectra reveals there must be more than two types of LH2 complexes present. Purified HL (high-light) and LL (low-light) LH2 complexes have mixed apoprotein compositions. The HL complexes contain PucAB a and PucAB b apoproteins. The LL complexes contain PucAB a, PucAB d and PucB b-only apoproteins. This mixed apoprotein composition can explain their resonance Raman spectra.

Authors:
 [1];  [2];  [3];  [4];  [1];  [2];  [1]
  1. Univ. of Glasgow, Scotland (United Kingdom). Inst. of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences
  2. Washington Univ., St. Louis, MO (United States). Depts. of Biology and Chemistry
  3. Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA-Saclay), Gif-sur-Yvette (France). Inst. de Biologie et Technologies de Saclay et CNRS
  4. Univ. of Glasgow, Scotland (United Kingdom). Dept. of Chemistry, WestChem
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC); Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1065582
DOE Contract Number:  
SC0001035; ANR-07-CEXC-009
Resource Type:
Journal Article
Journal Name:
Biochemical Journal
Additional Journal Information:
Journal Volume: 440; Journal Issue: 1; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 0264-6021
Publisher:
Biochemical Society
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; solar (fuels); photosynthesis (natural and artificial); biofuels (including algae and biomass); bio-inspired; charge transport; membrane; synthesis (novel materials); synthesis (self-assembly)

Citation Formats

Brotosudarmo, Tatas H. P., Collins, Aaron M., Gall, Andrew, Roszak, Aleksander W., Gardiner, Alastair T., Blankenship, Robert E., and Cogdell, Richard J. The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium. United States: N. p., 2011. Web. doi:10.1042/BJ20110575.
Brotosudarmo, Tatas H. P., Collins, Aaron M., Gall, Andrew, Roszak, Aleksander W., Gardiner, Alastair T., Blankenship, Robert E., & Cogdell, Richard J. The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium. United States. doi:10.1042/BJ20110575.
Brotosudarmo, Tatas H. P., Collins, Aaron M., Gall, Andrew, Roszak, Aleksander W., Gardiner, Alastair T., Blankenship, Robert E., and Cogdell, Richard J. Tue . "The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium". United States. doi:10.1042/BJ20110575.
@article{osti_1065582,
title = {The light intensity under which cells are grown controls the type of peripheral light-harvesting complexes that are assembled in a purple photosynthetic bacterium},
author = {Brotosudarmo, Tatas H. P. and Collins, Aaron M. and Gall, Andrew and Roszak, Aleksander W. and Gardiner, Alastair T. and Blankenship, Robert E. and Cogdell, Richard J.},
abstractNote = {The differing composition of LH2 (peripheral light-harvesting) complexes present in Rhodopseudomonas palustris 2.1.6 have been investigated when cells are grown under progressively decreasing light intensity. Analysis of the absorption spectra reveals there must be more than two types of LH2 complexes present. Purified HL (high-light) and LL (low-light) LH2 complexes have mixed apoprotein compositions. The HL complexes contain PucABa and PucABb apoproteins. The LL complexes contain PucABa, PucABd and PucBb-only apoproteins. This mixed apoprotein composition can explain their resonance Raman spectra.},
doi = {10.1042/BJ20110575},
journal = {Biochemical Journal},
issn = {0264-6021},
number = 1,
volume = 440,
place = {United States},
year = {2011},
month = {11}
}