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Title: Low light adaptation: Energy transfer processes in different types of light harvesting complexes from Rhodopseudomonas palustris

Abstract

Energy transfer processes in photosynthetic light harvesting 2 (LH2) complexes isolated from purple bacterium Rhodopseudomonas palustris grown at different light intensities were studied by ground state and transient absorption spectroscopy. The decomposition of ground state absorption spectra shows contributions from B800 and B850 bacteriochlorophyll (BChl) a rings, the latter component splitting into a low energy and a high energy band in samples grown under low light (LL) conditions. A spectral analysis reveals strong inhomogeneity of the B850 excitons in the LL samples that is well reproduced by an exponential-type distribution. Transient spectra show a bleach of both the low energy and high energy bands, together with the respective blue-shifted exciton-to-biexciton transitions. The different spectral evolutions were analyzed by a global fitting procedure. Energy transfer from B800 to B850 occurs in a mono-exponential process and the rate of this process is only slightly reduced in LL compared to high light samples. In LL samples, spectral relaxation of the B850 exciton follows strongly nonexponential kinetics that can be described by a reduction of the bleach of the high energy excitonic component and a red-shift of the low energetic one. We explain these spectral changes by picosecond exciton relaxation caused by a smallmore » coupling parameter of the excitonic splitting of the BChl a molecules to the surrounding bath. The splitting of exciton energy into two excitonic bands in LL complex is most probably caused by heterogenous composition of LH2 apoproteins that gives some of the BChls in the B850 ring B820-like site energies, and causes a disorder in LH2 structure.« less

Authors:
; ; ; ; ; ; ;
Research Org.:
Energy Frontier Research Centers (EFRC); Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1065058
DOE Contract Number:  
SC0001035
Resource Type:
Journal Article
Journal Name:
Biophys. J.
Additional Journal Information:
Journal Volume: 97; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; solar (fuels), photosynthesis (natural and artificial), biofuels (including algae and biomass), bio-inspired, charge transport, membrane, synthesis (novel materials), synthesis (self-assembly)

Citation Formats

Moulisova, Vladimira, Luer, Larry, Hoseinkhani, Sajjad, Brotosudarmo, Tatas H.P., Collins, Aaron M., Lanzani, Guglielmo, Blankenship, R. E., and Cogdell, Richard J. Low light adaptation: Energy transfer processes in different types of light harvesting complexes from Rhodopseudomonas palustris. United States: N. p., Web. doi:10.1016/j.bpj.2009.09.023.
Moulisova, Vladimira, Luer, Larry, Hoseinkhani, Sajjad, Brotosudarmo, Tatas H.P., Collins, Aaron M., Lanzani, Guglielmo, Blankenship, R. E., & Cogdell, Richard J. Low light adaptation: Energy transfer processes in different types of light harvesting complexes from Rhodopseudomonas palustris. United States. doi:10.1016/j.bpj.2009.09.023.
Moulisova, Vladimira, Luer, Larry, Hoseinkhani, Sajjad, Brotosudarmo, Tatas H.P., Collins, Aaron M., Lanzani, Guglielmo, Blankenship, R. E., and Cogdell, Richard J. . "Low light adaptation: Energy transfer processes in different types of light harvesting complexes from Rhodopseudomonas palustris". United States. doi:10.1016/j.bpj.2009.09.023.
@article{osti_1065058,
title = {Low light adaptation: Energy transfer processes in different types of light harvesting complexes from Rhodopseudomonas palustris},
author = {Moulisova, Vladimira and Luer, Larry and Hoseinkhani, Sajjad and Brotosudarmo, Tatas H.P. and Collins, Aaron M. and Lanzani, Guglielmo and Blankenship, R. E. and Cogdell, Richard J},
abstractNote = {Energy transfer processes in photosynthetic light harvesting 2 (LH2) complexes isolated from purple bacterium Rhodopseudomonas palustris grown at different light intensities were studied by ground state and transient absorption spectroscopy. The decomposition of ground state absorption spectra shows contributions from B800 and B850 bacteriochlorophyll (BChl) a rings, the latter component splitting into a low energy and a high energy band in samples grown under low light (LL) conditions. A spectral analysis reveals strong inhomogeneity of the B850 excitons in the LL samples that is well reproduced by an exponential-type distribution. Transient spectra show a bleach of both the low energy and high energy bands, together with the respective blue-shifted exciton-to-biexciton transitions. The different spectral evolutions were analyzed by a global fitting procedure. Energy transfer from B800 to B850 occurs in a mono-exponential process and the rate of this process is only slightly reduced in LL compared to high light samples. In LL samples, spectral relaxation of the B850 exciton follows strongly nonexponential kinetics that can be described by a reduction of the bleach of the high energy excitonic component and a red-shift of the low energetic one. We explain these spectral changes by picosecond exciton relaxation caused by a small coupling parameter of the excitonic splitting of the BChl a molecules to the surrounding bath. The splitting of exciton energy into two excitonic bands in LL complex is most probably caused by heterogenous composition of LH2 apoproteins that gives some of the BChls in the B850 ring B820-like site energies, and causes a disorder in LH2 structure.},
doi = {10.1016/j.bpj.2009.09.023},
journal = {Biophys. J.},
number = ,
volume = 97,
place = {United States},
year = {},
month = {}
}