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Title: Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park

Abstract

Geothermal systems in Yellowstone National Park (YNP) provide an outstanding opportunity to understand the origin and evolution of metabolic processes necessary for life in extreme environments including low pH, high temperature, low oxygen and elevated concentrations of reduced iron. Previous phylogenetic studies of acidic ferric iron mats from YNP have revealed considerable diversity of uncultivated and undescribed archaea. The goal of this study was to obtain replicate de novo genome assemblies for a dominant archaeal population inhabiting acidic iron oxide mats in YNP. Detailed analysis of conserved ribosomal and informational processing genes indicate that the replicate assemblies represent a new phylum-level lineage referred to here as 'novel archaeal group 1 (NAG1)'. The NAG1 organisms contain pathways necessary for the catabolism of peptides and complex carbohydrates as well as a bacterial-like Form I CO dehydrogenase complex likely used for energy conservation. Moreover, this novel population contains genes involved in metabolism of oxygen including a Type A heme copper oxidase, a bd-type terminal oxidase and a putative oxygen sensing protoglobin. NAG1 has a variety of unique bacterial-like cofactor biosynthesis and transport genes and a Type3-like CRISPR system. Discovery of NAG1 is critical to our understanding of microbial community structure and function inmore » extant thermophilic iron mats of YNP, and will provide insight regarding the evolution of Archaea in early Earth environments that may have important analogues active in YNP today.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1064570
Report Number(s):
PNNL-SA-85899
KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
The ISME Journal, 7(3):622-634
Additional Journal Information:
Journal Name: The ISME Journal, 7(3):622-634
Country of Publication:
United States
Language:
English
Subject:
extremophiles; geothermal; Yellowstone National Park; heme copper oxidase; carbon monoxide

Citation Formats

Kozubal, Mark, Romine, Margaret F, Jennings, Ryan, Jay, Z, Tringe, Susannah G, Rusch, Douglas B, Beam, Jake, McCue, Lee Ann, and Inskeep, William P. Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park. United States: N. p., 2013. Web. doi:10.1038/ismej.2012.132.
Kozubal, Mark, Romine, Margaret F, Jennings, Ryan, Jay, Z, Tringe, Susannah G, Rusch, Douglas B, Beam, Jake, McCue, Lee Ann, & Inskeep, William P. Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park. United States. https://doi.org/10.1038/ismej.2012.132
Kozubal, Mark, Romine, Margaret F, Jennings, Ryan, Jay, Z, Tringe, Susannah G, Rusch, Douglas B, Beam, Jake, McCue, Lee Ann, and Inskeep, William P. Fri . "Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park". United States. https://doi.org/10.1038/ismej.2012.132.
@article{osti_1064570,
title = {Geoarchaeota: a new candidate phylum in the Archaea from high-temperature acidic iron mats in Yellowstone National Park},
author = {Kozubal, Mark and Romine, Margaret F and Jennings, Ryan and Jay, Z and Tringe, Susannah G and Rusch, Douglas B and Beam, Jake and McCue, Lee Ann and Inskeep, William P},
abstractNote = {Geothermal systems in Yellowstone National Park (YNP) provide an outstanding opportunity to understand the origin and evolution of metabolic processes necessary for life in extreme environments including low pH, high temperature, low oxygen and elevated concentrations of reduced iron. Previous phylogenetic studies of acidic ferric iron mats from YNP have revealed considerable diversity of uncultivated and undescribed archaea. The goal of this study was to obtain replicate de novo genome assemblies for a dominant archaeal population inhabiting acidic iron oxide mats in YNP. Detailed analysis of conserved ribosomal and informational processing genes indicate that the replicate assemblies represent a new phylum-level lineage referred to here as 'novel archaeal group 1 (NAG1)'. The NAG1 organisms contain pathways necessary for the catabolism of peptides and complex carbohydrates as well as a bacterial-like Form I CO dehydrogenase complex likely used for energy conservation. Moreover, this novel population contains genes involved in metabolism of oxygen including a Type A heme copper oxidase, a bd-type terminal oxidase and a putative oxygen sensing protoglobin. NAG1 has a variety of unique bacterial-like cofactor biosynthesis and transport genes and a Type3-like CRISPR system. Discovery of NAG1 is critical to our understanding of microbial community structure and function in extant thermophilic iron mats of YNP, and will provide insight regarding the evolution of Archaea in early Earth environments that may have important analogues active in YNP today.},
doi = {10.1038/ismej.2012.132},
url = {https://www.osti.gov/biblio/1064570}, journal = {The ISME Journal, 7(3):622-634},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {3}
}