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Title: Increased Production of Fatty Acids and Triglycerides in Aspergillus oryzae by Enhancing Expressions of Fatty Acid Synthesis-Related Genes

Abstract

Microbial production of fats and oils is being developedas a means of converting biomass to biofuels. Here we investigate enhancing expression of enzymes involved in the production of fatty acids and triglycerides as a means to increase production of these compounds in Aspergillusoryzae. Examination of the A.oryzaegenome demonstrates that it contains twofatty acid synthases and several other genes that are predicted to be part of this biosynthetic pathway. We enhancedthe expressionof fatty acid synthesis-related genes by replacing their promoters with thepromoter fromthe constitutively highly expressedgene tef1. We demonstrate that by simply increasing the expression of the fatty acid synthasegenes we successfullyincreasedtheproduction of fatty acids and triglyceridesby more than two fold. Enhancement of expression of the fatty acid pathway genes ATP-citrate lyase and palmitoyl-ACP thioesteraseincreasedproductivity to a lesser extent.Increasing expression ofacetyl-CoA carboxylase caused no detectable change in fatty acid levels. Increases in message level for each gene were monitored usingquantitative real-time RT-PCR. Our data demonstrates that a simple increase in the abundance of fatty acid synthase genes can increase the detectable amount of fatty acids.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1060111
Report Number(s):
PNNL-SA-86701
BM0101020
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied Microbiology and Biotechnology, 97(1):269-281
Country of Publication:
United States
Language:
English
Subject:
Fatty acid,Triglyceride, Biofuel, Fungi, Aspergillus oryzae

Citation Formats

Tamano, Koichi, Bruno, Kenneth S., Karagiosis, Sue A., Culley, David E., Deng, Shuang, Collett, James R., Umemura, Myco, Koike, Hideaki, Baker, Scott E., and Machida, Masa. Increased Production of Fatty Acids and Triglycerides in Aspergillus oryzae by Enhancing Expressions of Fatty Acid Synthesis-Related Genes. United States: N. p., 2013. Web. doi:10.1007/s00253-012-4193-y.
Tamano, Koichi, Bruno, Kenneth S., Karagiosis, Sue A., Culley, David E., Deng, Shuang, Collett, James R., Umemura, Myco, Koike, Hideaki, Baker, Scott E., & Machida, Masa. Increased Production of Fatty Acids and Triglycerides in Aspergillus oryzae by Enhancing Expressions of Fatty Acid Synthesis-Related Genes. United States. doi:10.1007/s00253-012-4193-y.
Tamano, Koichi, Bruno, Kenneth S., Karagiosis, Sue A., Culley, David E., Deng, Shuang, Collett, James R., Umemura, Myco, Koike, Hideaki, Baker, Scott E., and Machida, Masa. Tue . "Increased Production of Fatty Acids and Triglycerides in Aspergillus oryzae by Enhancing Expressions of Fatty Acid Synthesis-Related Genes". United States. doi:10.1007/s00253-012-4193-y.
@article{osti_1060111,
title = {Increased Production of Fatty Acids and Triglycerides in Aspergillus oryzae by Enhancing Expressions of Fatty Acid Synthesis-Related Genes},
author = {Tamano, Koichi and Bruno, Kenneth S. and Karagiosis, Sue A. and Culley, David E. and Deng, Shuang and Collett, James R. and Umemura, Myco and Koike, Hideaki and Baker, Scott E. and Machida, Masa},
abstractNote = {Microbial production of fats and oils is being developedas a means of converting biomass to biofuels. Here we investigate enhancing expression of enzymes involved in the production of fatty acids and triglycerides as a means to increase production of these compounds in Aspergillusoryzae. Examination of the A.oryzaegenome demonstrates that it contains twofatty acid synthases and several other genes that are predicted to be part of this biosynthetic pathway. We enhancedthe expressionof fatty acid synthesis-related genes by replacing their promoters with thepromoter fromthe constitutively highly expressedgene tef1. We demonstrate that by simply increasing the expression of the fatty acid synthasegenes we successfullyincreasedtheproduction of fatty acids and triglyceridesby more than two fold. Enhancement of expression of the fatty acid pathway genes ATP-citrate lyase and palmitoyl-ACP thioesteraseincreasedproductivity to a lesser extent.Increasing expression ofacetyl-CoA carboxylase caused no detectable change in fatty acid levels. Increases in message level for each gene were monitored usingquantitative real-time RT-PCR. Our data demonstrates that a simple increase in the abundance of fatty acid synthase genes can increase the detectable amount of fatty acids.},
doi = {10.1007/s00253-012-4193-y},
journal = {Applied Microbiology and Biotechnology, 97(1):269-281},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2013},
month = {Tue Jan 01 00:00:00 EST 2013}
}