Genetically encoded fluorescent coumarin amino acids

Wang, Jiangyun; Xie, Jianming; Schultz, Peter G

Title: Genetically encoded fluorescent coumarin amino acids

Patent · Tue Jun 05 00:00:00 EDT 2012

OSTI ID:1055725

Wang, Jiangyun ^[1]; Xie, Jianming ^[1]; Schultz, Peter G ^[2]

San Diego, CA
La Jolla, CA

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.

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Research Organization:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-03ER46051

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Number(s):: 8,192,952

Application Number:: 12/227,383

OSTI ID:: 1055725

Country of Publication:: United States

Language:: English

References (17)

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A chemical toolkit for proteins — an expanded genetic code Xie, Jianming; Schultz, Peter G. Nature Reviews Molecular Cell Biology, Vol. 7, Issue 10 https://doi.org/10.1038/nrm2005	journal	August 2006
An expanding genetic code Xie, Jianming; Schultz, Peter G. Methods, Vol. 36, Issue 3, p. 227-238 https://doi.org/10.1016/j.ymeth.2005.04.010	journal	July 2005
A Genetically Encoded Fluorescent Amino Acid Wang, Jiangyun; Xie, Jianming; Schultz, Peter G. Journal of the American Chemical Society, Vol. 128, Issue 27 https://doi.org/10.1021/ja062666k	journal	July 2006
Expanding the genetic code Wang, Lei; Schultz, Peter G. Chemical Communications, Issue 1 https://doi.org/10.1039/b108185n	journal	December 2001
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Expanding the Genetic Code Wang, Lei; Schultz, Peter G. Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66 https://doi.org/10.1002/anie.200460627	journal	January 2005
A Very Short Route to Enantiomerically Pure Coumarin-Bearing Fluorescent Amino Acids Brun, Marie-Priscille; Bischoff, Laurent; Garbay, Christiane Angewandte Chemie International Edition, Vol. 43, Issue 26 https://doi.org/10.1002/anie.200454116	journal	June 2004
Programming peptidomimetic syntheses by translating genetic codes designed de novo Forster, A. C.; Tan, Z.; Nalam, M. N. L. Proceedings of the National Academy of Sciences, Vol. 100, Issue 11, p. 6353-6357 https://doi.org/10.1073/pnas.1132122100	journal	May 2003
Expanding the Genetic code Wang, Lei; Xie, Jianming; Schultz, Peter G. Annual Review of Biophysics and Biomolecular Structure, Vol. 35, Issue 1 https://doi.org/10.1146/annurev.biophys.35.101105.121507	journal	June 2006
Expanding tRNA recognition of a tRNA synthetase by a single amino acid change Feng, L.; Tumbula-Hansen, D.; Toogood, H. Proceedings of the National Academy of Sciences, Vol. 100, Issue 10, p. 5676-5681 https://doi.org/10.1073/pnas.0631525100	journal	May 2003
Adding amino acids to the genetic repertoire Xie, Jianming; Schultz, Peter G. Current Opinion in Chemical Biology, Vol. 9, Issue 6, p. 548-554 https://doi.org/10.1016/j.cbpa.2005.10.011	journal	December 2005
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An Expanded Eukaryotic Genetic Code Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher Science, Vol. 301, Issue 5635, p. 964-967 https://doi.org/10.1126/science.1084772	journal	August 2003
A general approach for the generation of orthogonal tRNAs Wang, Lei; Schultz, Peter G. Chemistry & Biology, Vol. 8, Issue 9, p. 883-890 https://doi.org/10.1016/S1074-5521(01)00063-1	journal	September 2001
Expanding the Genetic Code of Escherichia coli Wang, L.; Brock, A.; Herberich, B. Science, Vol. 292, Issue 5516, p. 498-500 https://doi.org/10.1126/science.1060077	journal	April 2001
Expansion of the genetic code: Site-directed p-fluoro-phenylalanine incorporation in Escherichia coli Furter, Rolf Protein Science, Vol. 7, Issue 2, p. 419-426 https://doi.org/10.1002/pro.5560070223	journal	February 1998

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