Systems for the expression of orthogonal translation components eubacterial host cells

Ryu, Youngha; Schultz, Peter G

Title: Systems for the expression of orthogonal translation components eubacterial host cells

Patent · Tue Jun 12 00:00:00 EDT 2012

OSTI ID:1055692

Ryu, Youngha ^[1]; Schultz, Peter G ^[2]

San Diego, CA
La Jolla, CA

The invention relates to compositions and methods for the in vivo production of polypeptides comprising one or more unnatural amino acids. Specifically, the invention provides plasmid systems for the efficient eubacterial expression of polypeptides comprising one or more unnatural amino acids at genetically-programmed positions.

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Research Organization:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-03ER46051

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Number(s):: 8,198,044

Application Number:: 12/224,773

OSTI ID:: 1055692

Country of Publication:: United States

Language:: English

References (17)

A Genetically Encoded Photocaged Amino Acid Wu, Ning; Deiters, Alexander; Cropp, T. Ashton Journal of the American Chemical Society, Vol. 126, Issue 44 https://doi.org/10.1021/ja040175z	journal	November 2004
An expanding genetic code Xie, Jianming; Schultz, Peter G. Methods, Vol. 36, Issue 3, p. 227-238 https://doi.org/10.1016/j.ymeth.2005.04.010	journal	July 2005
Genomic organization and physical mapping of the transfer RNA genes in Escherichia coli K12 Komine, Yuriko; Adachi, Toshiharu; Inokuchi, Hachiro Journal of Molecular Biology, Vol. 212, Issue 4 https://doi.org/10.1016/0022-2836(90)90224-A	journal	April 1990
Structure of an Escherichia coli tRNA operon containing linked genes for arginine, histidine, leucine, and proline tRNAs. Hsu, L. M.; Klee, H. J.; Zagorski, J. Journal of Bacteriology, Vol. 158, Issue 3 https://doi.org/10.1128/JB.158.3.934-942.1984	journal	January 1984
Expanding the Genetic Code Wang, Lei; Schultz, Peter G. Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66 https://doi.org/10.1002/anie.200460627	journal	January 2005
Expanding the Genetic code Wang, Lei; Xie, Jianming; Schultz, Peter G. Annual Review of Biophysics and Biomolecular Structure, Vol. 35, Issue 1 https://doi.org/10.1146/annurev.biophys.35.101105.121507	journal	June 2006
Site-Specific Incorporation of a Redox-Active Amino Acid into Proteins Alfonta, Lital; Zhang, Zhiwen; Uryu, Sean Journal of the American Chemical Society, Vol. 125, Issue 48, p. 14662-14663 https://doi.org/10.1021/ja038242x	journal	December 2003
Structure and transcription of the tRNA ₁ ^Pro gene from Escherichia coli Kuchino, Yoshiyuki; Mori, Fumiko; Nishimura, Susumu Nucleic Acids Research, Vol. 13, Issue 9 https://doi.org/10.1093/nar/13.9.3213	journal	January 1985
Addition of the keto functional group to the genetic code of Escherichia coli Wang, L.; Zhang, Z.; Brock, A. Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61 https://doi.org/10.1073/pnas.0234824100	journal	December 2002
Adding amino acids to the genetic repertoire Xie, Jianming; Schultz, Peter G. Current Opinion in Chemical Biology, Vol. 9, Issue 6, p. 548-554 https://doi.org/10.1016/j.cbpa.2005.10.011	journal	December 2005
A New Strategy for the Synthesis of Glycoproteins Zhang, Z. Science, Vol. 303, Issue 5656 https://doi.org/10.1126/science.1089509	journal	January 2004
FIS activates sequential steps during transcription initiation at a stable RNA promoter Muskhelishvili, G. The EMBO Journal, Vol. 16, Issue 12 https://doi.org/10.1093/emboj/16.12.3655	journal	June 1997
Efficient incorporation of unnatural amino acids into proteins in Escherichia coli Ryu, Youngha; Schultz, Peter G. Nature Methods, Vol. 3, Issue 4 https://doi.org/10.1038/nmeth864	journal	March 2006
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli Chin, J. W.; Martin, A. B.; King, D. S. Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024 https://doi.org/10.1073/pnas.172226299	journal	August 2002
A general approach for the generation of orthogonal tRNAs Wang, Lei; Schultz, Peter G. Chemistry & Biology, Vol. 8, Issue 9, p. 883-890 https://doi.org/10.1016/S1074-5521(01)00063-1	journal	September 2001
Expanding the Genetic Code of Escherichia coli Wang, L.; Brock, A.; Herberich, B. Science, Vol. 292, Issue 5516, p. 498-500 https://doi.org/10.1126/science.1060077	journal	April 2001
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells Sakamoto, K. Nucleic Acids Research, Vol. 30, Issue 21, p. 4692-4699 https://doi.org/10.1093/nar/gkf589	journal	November 2002

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