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Title: Atomic force microscopy of silica nanoparticles and carbon nanohorns in macrophages and red blood cells

Abstract

The emerging interest in understanding the interactions of nanomaterial with biological systems necessitates imaging tools that capture the spatial and temporal distributions and attributes of the resulting nano-bil amalgam. Studies targeting organ specific response and/ot nanoparticle-specific system toxicity would be profoundly benefited from tools that would allow imaging and tracking of in-vivo or in-vitro processes and particle-fate studies. Recently we demonstrated that mode systhesizing atomic force microscopy (MSAFM) can provide subsurface nanoscale informations on the mechanical properties of materials at the nanoscale. However, the underlying mechanism of this imaging methodology is currently subject to theoretical and experimental investigation. In this paper we present further analysis by investigating tip-sample excitation forces associated with nanomechanical image formation. Images and force curves acquired under various operational frequencies and amplitudes are presented. We examine samples of mouse cells, where buried distributions of single-walled carbon nanohorns and silica nanoparticles are visualized.

Authors:
 [1];  [1];  [1];  [1]
  1. ORNL
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program
OSTI Identifier:
1055201
DOE Contract Number:  
DE-AC05-00OR22725
Resource Type:
Journal Article
Journal Name:
Ultramicroscopy
Additional Journal Information:
Journal Volume: 110; Journal Issue: 6; Journal ID: ISSN 0304-3991
Country of Publication:
United States
Language:
English
Subject:
Atomic force microscopy; Mode synthesizing atomic force microscopy; Raman spectroscopy; Nanoparticles; Nanomechanics; Force curves; Cells

Citation Formats

Tetard, Laurene, Passian, Ali, Farahi, R H, and Thundat, Thomas George. Atomic force microscopy of silica nanoparticles and carbon nanohorns in macrophages and red blood cells. United States: N. p., 2010. Web. doi:10.1016/j.ultramic.2010.02.015.
Tetard, Laurene, Passian, Ali, Farahi, R H, & Thundat, Thomas George. Atomic force microscopy of silica nanoparticles and carbon nanohorns in macrophages and red blood cells. United States. doi:10.1016/j.ultramic.2010.02.015.
Tetard, Laurene, Passian, Ali, Farahi, R H, and Thundat, Thomas George. Fri . "Atomic force microscopy of silica nanoparticles and carbon nanohorns in macrophages and red blood cells". United States. doi:10.1016/j.ultramic.2010.02.015.
@article{osti_1055201,
title = {Atomic force microscopy of silica nanoparticles and carbon nanohorns in macrophages and red blood cells},
author = {Tetard, Laurene and Passian, Ali and Farahi, R H and Thundat, Thomas George},
abstractNote = {The emerging interest in understanding the interactions of nanomaterial with biological systems necessitates imaging tools that capture the spatial and temporal distributions and attributes of the resulting nano-bil amalgam. Studies targeting organ specific response and/ot nanoparticle-specific system toxicity would be profoundly benefited from tools that would allow imaging and tracking of in-vivo or in-vitro processes and particle-fate studies. Recently we demonstrated that mode systhesizing atomic force microscopy (MSAFM) can provide subsurface nanoscale informations on the mechanical properties of materials at the nanoscale. However, the underlying mechanism of this imaging methodology is currently subject to theoretical and experimental investigation. In this paper we present further analysis by investigating tip-sample excitation forces associated with nanomechanical image formation. Images and force curves acquired under various operational frequencies and amplitudes are presented. We examine samples of mouse cells, where buried distributions of single-walled carbon nanohorns and silica nanoparticles are visualized.},
doi = {10.1016/j.ultramic.2010.02.015},
journal = {Ultramicroscopy},
issn = {0304-3991},
number = 6,
volume = 110,
place = {United States},
year = {2010},
month = {1}
}