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Title: Fluorescence Axial Localization with Nanometer Accuracy and Precision

Abstract

We describe a new technique, standing wave axial nanometry (SWAN), to image the axial location of a single nanoscale fluorescent object with sub-nanometer accuracy and 3.7 nm precision. A standing wave, generated by positioning an atomic force microscope tip over a focused laser beam, is used to excite fluorescence; axial position is determined from the phase of the emission intensity. We use SWAN to measure the orientation of single DNA molecules of different lengths, grafted on surfaces with different functionalities.

Authors:
; ;
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1052066
Report Number(s):
IS-J 7757
DOE Contract Number:  
DE-AC02-07CH11358
Resource Type:
Journal Article
Journal Name:
Nano Letters
Additional Journal Information:
Journal Volume: 12; Journal Issue: 7
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Li, Hui, Yen, Chi-Fu, and Sivasankar, Sanjeevi. Fluorescence Axial Localization with Nanometer Accuracy and Precision. United States: N. p., 2012. Web. doi:10.1021/nl301542c.
Li, Hui, Yen, Chi-Fu, & Sivasankar, Sanjeevi. Fluorescence Axial Localization with Nanometer Accuracy and Precision. United States. doi:10.1021/nl301542c.
Li, Hui, Yen, Chi-Fu, and Sivasankar, Sanjeevi. Fri . "Fluorescence Axial Localization with Nanometer Accuracy and Precision". United States. doi:10.1021/nl301542c.
@article{osti_1052066,
title = {Fluorescence Axial Localization with Nanometer Accuracy and Precision},
author = {Li, Hui and Yen, Chi-Fu and Sivasankar, Sanjeevi},
abstractNote = {We describe a new technique, standing wave axial nanometry (SWAN), to image the axial location of a single nanoscale fluorescent object with sub-nanometer accuracy and 3.7 nm precision. A standing wave, generated by positioning an atomic force microscope tip over a focused laser beam, is used to excite fluorescence; axial position is determined from the phase of the emission intensity. We use SWAN to measure the orientation of single DNA molecules of different lengths, grafted on surfaces with different functionalities.},
doi = {10.1021/nl301542c},
journal = {Nano Letters},
number = 7,
volume = 12,
place = {United States},
year = {2012},
month = {6}
}